LookOut® Mycoplasma PCR Detection Kit MP0035 from MilliporeSigma

Product Specs
ItemLookOut® Mycoplasma PCR Detection Kit
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMP0035
ApplicationsPCR, microbiology cell culture
Quantity1KT

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MilliporeSigma

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Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) CTLA-4 antibody-drug conjugate reveals autologous destruction of B-lymphocytes associated with regulatory T cell impairmentElifeDecember 21, 2023Musleh M. Muthana, Xuexiang Du, Mingyue Liu, Xu Wang, Wei Wu, Chunxia Ai, Lishan Su, Pan Zheng, Yang LiuPierce BCA Protein Assay Kit (Thermo Fisher Scientific/Pierce, 23227). LookOut Mycoplasama PCR Detection Kit (Sigma-Aldrich, MP0035). Flow antibodies eBioscience/ Thermo Fisher Scientific (San Diego, CA): APC-eFlour780 anti-mouse

(2) Development of adaptive anoikis resistance promotes metastasis that can be overcome by CDK8/19 Mediator kinase inhibitionbioRxivDecember 6, 2023Mehri Monavarian, Emily Faith Page, Resha Rajkarnikar, Asha Kumari, Liz Quintero Macias, Felipe Massicano, Nam Y Lee, Sarthak Sahoo, Nadine Hempel, Mohit Kumar Jolly, et al.tested frequently for mycoplasma contamination using LookOut® Mycoplasma PCR Detection Kit (cat no: MP0035-1KT, Millipore sigma). CAOV3, P76, P151, P201, P211, HEK293 were cultured in DMEM (contains high glucose,

(3) Systematic characterization of the HOXA9 downstream targets in MLL-r leukemia by noncoding CRISPR screensNature CommunicationsNovember 28, 2023Shaela Wright, Xujie Zhao, Wojciech Rosikiewicz, Shelby Mryncza, Judith Hyle, Wenjie Qi, Zhenling Liu, Siqi Yi, Yong Cheng, Beisi Xu, et al.lines used in this study were Mycoplasma-free examined by Lookout Mycoplasma PCR Detection Kit (Sigma, #MP0035) following the instruction. Vectors The Lenti-Cas9-Blast (Addgene #83480), Lenti-dCas9-KRAB-blast

(4) circ-hnRNPU inhibits NONO-mediated c-Myc transactivation and mRNA stabilization essential for glycosylation and cancer progressionJournal of Experimental & Clinical Cancer ResearchNovember 23, 2023Hongjun Li, Wanju Jiao, Jiyu Song, Jianqun Wang, Guo Chen, Dan Li, Xiaojing Wang, Banghe Bao, Xinyi Du, Yang Cheng, et al.aliquots. Mycoplasma contamination was regularly examined using Lookout Mycoplasma PCR Detection Kit (MP0035, Sigma, St. Louis, MO). Cell lines were cultured in RPMI 1640 medium supplied with 10% fetal bovine

(5) ATG9A regulates the dissociation of recycling endosomes from microtubules to form liquid influenza A virus inclusionsPLOS BiologyNovember 20, 2023Sílvia Vale-Costa, Temitope Akhigbe Etibor, Daniela Brás, Ana Laura Sousa, Mariana Ferreira, Gabriel G. Martins, Victor Hugo Mello, Maria João Amorimregularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma, D9307). Reverse genetics derived A/Puerto Rico/8/34 (PR8

(6) Discovery of New Anti-Cancer Agents against Patient-Derived Sorafenib-Resistant Papillary Thyroid CancerInternational Journal of Molecular SciencesNovember 16, 2023Yuna Kim, Hyeok Jun Yun, Kyung Hwa Choi, Chan Wung Kim, Jae Ha Lee, Raymond Weicker, Seok-Mo Kim, Ki Cheong Parkcontamination was checked for with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louis, MO, USA; MP0035). 4.5. mRNA-Seq Data We preprocessed the raw reads from the sequencer to remove low-quality and adapter

(7) m1A in CAG repeat RNA binds to TDP-43 and induces neurodegenerationNatureNovember 16, 2023Yuxiang Sun, Hui Dai, Xiaoxia Dai, Jiekai Yin, Yuxiang Cui, Xiaochuan Liu, Gwendolyn Gonzalez, Jun Yuan, Feng Tang, Nan Wang, et al.were confirmed to be free of mycoplasma contamination by using LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich). shRNAs against TRMT61A, TRMT61B and TRMT10C were designed and their sequences are listed

(8) DGAT inhibition at the post‐absorptive phase reduces plasma FA by increasing FA oxidationEMBO Molecular MedicineNovember 8, 2023Anand Kumar Sharma, Christian Wolfrumare tested for mycoplasma at regular intervals using LookOut® Mycoplasma PCR‐Detections‐Kit (Merck, MP0035‐1KT). No blinding or sample size estimate was done during the experiment or the analysis. No formal

(9) USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing SarcomaMolecular Cancer ResearchNovember 1, 2023Halle J. Mallard, Shibiao Wan, Prakriti Nidhi, Yvan D. Hanscom-Trofy, Bhopal Mohapatra, Nicholas T. Woods, Jose Antonio Lopez-Guerrero, Antonio Llombart-Bosch, Isidro Machado, Katia Scotlandi, et al.is performed at intervals of 30 to 45 days using the SIGMA Mycoplasma PCR detection kit (Catalog no. MP0035). Experiments were performed with cell lines passages 5–15 within 1 week of thawing. For replicate experiments,

(10) Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresseLifeOctober 30, 2023Jinyang Wang, Yaxin Wen, Wentao Zhao, Yan Zhang, Furong Lin, Cong Ouyang, Huihui Wang, Lizheng Yao, Huanhuan Ma, Yue Zhuo, et al.All cells tested negative for mycoplasma infection using a PCR-based Mycoplasma Detection Kit (Sigma, MP0035-1KT). Mouse embryonic fibroblast cells were isolated from the embryos of mice at 13.5 days post-coitum

(11) GREB1 isoform 4 is specifically transcribed by MITF and required for melanoma proliferationOncogeneOctober 13, 2023Koei Shinzawa, Shinji Matsumoto, Ryota Sada, Akikazu Harada, Kaori Saitoh, Keiko Kato, Satsuki Ikeda, Akiyoshi Hirayama, Kazunori Yokoi, Atsushi Tanemura, et al.lines were tested monthly for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich, MP0035, St Louis, MO, USA). Cell line identity was confirmed using STR profiling. RNA and ATAC sequences of

(12) A Carbonic Anhydrase IX/SLC1A5 Axis Regulates Glutamine Metabolism Dependent Ferroptosis in Hypoxic Tumor CellsMolecular Cancer TherapeuticsOctober 2, 2023Geetha Venkateswaran, Paul C. McDonald, Shawn C. Chafe, Wells S. Brown, Zachary J. Gerbec, Shannon J. Awrey, Seth J. Parker, Shoukat Dedhar(Genetica, Burlington, NC) and screened for mycoplasma using the LookOut Mycoplasma test (Sigma, catalog no. MP0035) or DNA staining test. The latest Mycoplasma testing for 4T1Luc and SUM159 using LookOut Mycoplasma

(13) A hybrid breast cancer/mesenchymal stem cell population enhances chemoresistance and metastasisJCI InsightSeptember 22, 2023Giuseppina Augimeri, Maria E. Gonzalez, Alessandro Paolì, Ahmad Eido, Yehyun Choi, Boris Burman, Sabra Djomehri, Santhosh Kumar Karthikeyan, Sooryanarayana Varambally, Johanna M. Buschhaus, et al.and were tested for mycoplasma infection using Sigma LookOut Mycoplasma PCR Detection Kit (catalog MP0035). BCCs and MSCs were labeled with GFP and DsRed, respectively. In another set of experiments, BCCs were

(14) Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistanceeLifeSeptember 22, 2023Rebecca E K Mandt, Madeline R Luth, Mark A Tye, Ralph Mazitschek, Sabine Ottilie, Elizabeth A Winzeler, Maria Jose Lafuente-Monasterio, Francisco Javier Gamo, Dyann F Wirth, Amanda K LukensTissue Kit,QIAGEN,Cat # 69556, Commercial assay or kit,LookOut Mycoplasma PCR Detection Kit,Sigma,Cat # MP0035, Commercial assay or kit,Phusion High-Fidelity PCR Master Mix,New England BioLabs,Cat No: M0531S, Commercial

(15) Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processesbioRxivSeptember 14, 2023Bruno A. Cisterna, Kristen Skruber, Makenzie L. Jane, Caleb I. Camesi, Ivan D. Nguyen, Peyton V. Warp, Joseph B. Black, Mitchell T. Butler, James E. Bear, Tracy-Ann Tracy-Ann, et al.incubator. Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich). Primary hippocampal neuron cultures Mice primary hippocampal neuronal cultures were

(16) Minichromosome maintenance proteins in lung adenocarcinoma: Clinical significance and therapeutic targetsFEBS Open BioSeptember 1, 2023Kengo Tanigawa, Yuya Tomioka, Shunsuke Misono, Shunichi Asai, Naoko Kikkawa, Yoko Hagihara, Takayuki Suetsugu, Hiromasa Inoue, Keiko Mizuno, Naohiko Sekiin A549 and H1299 cells was carried out using a LookOut Mycoplasma PCR Detection Kit (catalog no.: MP0035; Sigma‐Aldrich, MO, USA) and JumpStart Taq DNA Polymerase (catalog no.: D9307; Sigma‐Aldrich) according

(17) Renal FGF23 signaling depends on redox protein Memo1 and promotes orthovanadate-sensitive protein phosphotyrosyl phosphatase activityJournal of cell communication and signalingSeptember 1, 2023Bartos K, Ramakrishnan SK, Braga-Lagache S, Hänzi B, Durussel F, Prakash Sridharan A, Zhu Y, Sheehan D, Hynes NE, Bonny O, et al.HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr. Bettina Lorenz-Depiereux,

(18) LNS8801 inhibits Acute Myeloid Leukemia by Inducing the Production of Reactive Oxygen Species and Activating the Endoplasmic Reticulum Stress PathwayCancer Research CommunicationsAugust 18, 2023Inyoung Lee, Miriam Doepner, Jillian Weissenrieder, Ariana D. Majer, Sophia Mercado, Angela Estell, Christopher A. Natale, Pamela J. Sung, J. Kevin Foskett, Martin P. Carroll, et al.P/S). All cell lines were tested for Mycoplasma with the LookOut Mycoplasma PCR Detection Kit (Sigma MP0035). Reagents Functional necessity of the different caspases was tested using the pan-caspase inhibitor

(19) SARS-CoV-2 ORF3c impairs mitochondrial respiratory metabolism, oxidative stress, and autophagic fluxiScienceJuly 21, 2023Mozzi A, Oldani M, Forcella ME, Vantaggiato C, Cappelletti G, Pontremoli C, Valenti F, Forni D, Saresella M, Biasin M, et al.at 37°C in a humidified 5% CO2 incubator. All cell lines were tested for mycoplasma contamination (MP0035; Merck Life Science). Autophagy was induced by amino acid and serum starvation in Earle’s Balanced Salt

(20) FOXK2 amplification and overexpression promotes breast cancer development and chemoresistancebioRxivJune 2, 2023Yang Yu, Wen-Ming Cao, Feng Cheng, Zhongcheng Shi, Lili Han, Jin-Ling Yi, Edaise M da Silva, Higinio Dopeso, Hui Chen, Jianhua Yang, et al.Anderson Cancer Center. Mycoplasma testing was performed by LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich). Antibodies and inhibitors The antibody for FOXK2 (ab5298) was from Abcam Biotechnology

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LookOut® Mycoplasma PCR Detection Kit from MilliporeSigma

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LookOut® Mycoplasma PCR Detection Kit from MilliporeSigma

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