In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based TOX7 from MilliporeSigma

Product Specs
ItemIn Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberTOX7
Applicationsprotein quantification detection, cell analysis
Quantity1KT

Add to Compare List

MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Antiproliferative and Cytotoxic Properties of Propynoyl Betulin Derivatives against Human Ovarian Cancer Cells: In Vitro StudiesInternational Journal of Molecular SciencesNovember 18, 2023Ewa Chodurek, Arkadiusz Orchel, Paweł Gwiazdoń, Anna Kaps, Piotr Paduszyński, Marzena Jaworska-Kik, Elwira Chrobak, Ewa Bębenek, Stanisław Boryczka, Janusz Kasperczykof LDH into the culture medium using the In Vitro Toxicology Assay Kit, Lactic Dehydrogenase Based (TOX7; Sigma Aldrich) was assessed. SK-OV-3 and OVCAR-3 cells were plated and cultured in 96-well plates (Greiner

(2) Interrogating the Role of miR-125b and Its 3′isomiRs in Protection against HypoxiaInternational Journal of Molecular SciencesNovember 6, 2023Lee Lee Wong, Azizah Binti Fadzil, Qiying Chen, Miriam T. Rademaker, Christopher J. Charles, Arthur Mark Richards, Peipei Wanghypoxia, cell culture medium was collected for measurement of lactate dehydrogenase (LDH) release (TOX7, Sigma-Aldrich, St. Louis, MO, USA), an indicator of cell injury, and cells were counted (cell counting

(3) Ceramide Is Involved in Temozolomide Resistance in Human Glioblastoma U87MG Overexpressing EGFRInternational Journal of Molecular SciencesOctober 20, 2023Rosaria Bassi, Michele Dei Cas, Cristina Tringali, Federica Compostella, Rita Paroni, Paola Giussanimeasures the extracellular release of lactate dehydrogenase (LDH). For this purpose, a commercial kit (TOX7, Sigma-Aldrich, Darmstadt, Germany) was used, which is based on the reduction of NAD by LDH. The resulting

(4) A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis AssayInt J Mol SciAugust 16, 2023Juan Luis de la Fuente-Jiménez, César Iván Rodríguez-Rivas, Irma Beatriz Mitre-Aguilar, Andrea Torres-Copado, Eric Alejandro García-López, José Herrera-Celis, María Goretti Arvizu-Espinosa, Marco Antonio Garza-Navarro, Luis Gerardo Arriaga, Janet Ledesma García, et al.was performed as is described in In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based, reference TOX7-1KT (Sigma-Aldrich), which can be explained as follows: ss mentioned above in the MTT and Crystal violet

(5) 3D printing of piezoelectric and bioactive barium titanate-bioactive glass scaffolds for bone tissue engineeringMaterials Today BioAugust 1, 2023Christian Polley, Thomas Distler, Caroline Scheufler, Rainer Detsch, Henrik Lund, Armin Springer, Dominik Schneidereit, Oliver Friedrich, Aldo R. Boccaccini, Hermann SeitzBaTiO3/BG scaffolds, extracellular LDH release was determined from cell culture supernatants using the TOX7 in vitro toxicity kit (Sigma-Aldrich). Aliquots of 500 μl were removed from MC3T3-E1 cells cultured

(6) Time- and Concentration-Dependent Adverse Effects of Paclitaxel on Non-Neuronal Cells in Rat Primary Dorsal Root GangliaToxicsJuly 4, 2023Amira Elfarnawany, Faramarz Dehghanirelease (positive LDH control, 100 % cell death), 1:10 of the LDH lysis kit (LDH, Sigma Aldrich, cat. No. TOX7, St. Louis, MO, USA) was added to some wells and incubated for 45 min. According to the manufacturer’s

(7) SARS-CoV-2 drives NLRP3 inflammasome activation in human microglia through spike proteinMolecular psychiatryJuly 1, 2023Albornoz EA, Amarilla AA, Modhiran N, Parker S, Li XX, Wijesundara DK, Aguado J, Zamora AP, McMillan CLD, Liang B, et al.each treatment, supernatants were collected and LDH release was quantified using an LDH assay kit (TOX7-Sigma) as per the manufacturer’s instructions. Caspase-1 dependent LDH release that was inhibited by

(8) Terahertz Spectroscopy Sheds Light on Real‐Time Exchange Kinetics Occurring through Plasma Membrane during Photodynamic Therapy TreatmentAdvanced ScienceJune 1, 2023Xiujun Zheng, Blandine Lordon, Anne‐Françoise Mingotaud, Patricia Vicendo, Rachel Brival, Isabelle Fourquaux, Laure Gibot, Guilhem Gallotfunction of the amount of cytoplasmic LDH released into the medium. For that purpose, LDH Assay (Sigma, #TOX7, St Quentin Fallavier, France) was performed according to the manufacturer's protocol. Note that to

(9) Transcriptome Analysis of Co-Cultures of THP-1 Human Macrophages with Inactivated Germinated Trichophyton rubrum ConidiaJournal of FungiMay 12, 2023Bruna Aline Cantelli, Gabriela Gonzalez Segura, Tamires Aparecida Bitencourt, Mariana Heinzen de Abreu, Monise Fazolin Petrucelli, Kamila Peronni, Pablo Rodrigo Sanches, Rene Oliveira Beleboni, Wilson Araújo da Silva Junior, Nilce Maria Martinez-Rossi, et al.dehydrogenase (LDH) was analyzed after different incubation periods (5, 8, 12, 24, and 48 h) [29] using the TOX7 kit (Sigma-Aldrich) according to the manufacturer protocol, as described previously [20]. 2.6. Quantification

(10) Rosiglitazone reduces diabetes angiopathy by inhibiting mitochondrial dysfunction dependent on regulating HSP22 expressioniScienceApril 21, 2023Lingling Yu, Shengsong Chen, Qian Liang, Chahua Huang, Weifang Zhang, Longlong Hu, Yun Yu, Liang Liu, Xiaoshu Cheng, Huihui BaoLipofectamine 3000,Invitrogen,L3000150 Cell Counting Kit-8 (CCK8),Dojindo,CK04 Lactic Dehydrogenase-based Toxicology Assay Kit,Sigma-Aldrich,TOX7-1KT TRIzol reagent,Invitrogen,15596026 PrimeScript™ RT reagent Kit,Takara,RR047A TB Green® Premix

(11) Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and MicromotionBiosensors (Basel)February 28, 2023Theresa M. Curtis, Annabella M. Nilon, Anthony J. Greenberg, Matthew Besner, Jacob J. Scibek, Jennifer A. Nichols, Janet L. Huiecellular release was performed using the In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based (#TOX7; Sigma-Aldrich, Inc., St. Louis, MO, USA). HUVECs and HaCaT cells were seeded at 2 × 104 and 4 × 104

(12) In vitro cytocompatibility and antibacterial studies on biodegradable Zn alloys supplemented by a critical assessment of direct contact cytotoxicity assayJournal of Biomedical Materials Research Part B: Applied BiomaterialsFebruary 1, 2023Maria Wątroba, Wiktor Bednarczyk, Piotr K. Szewczyk, Jakub Kawałko, Krzysztof Mech, Alina Grünewald, Irem Unalan, Nicola Taccardi, Gabriela Boelter, Manuel Banzhaf, et al.LDH release level was measured after 1‐day of incubation using an LDH‐activity quantification kit (TOX7, Sigma‐Aldrich). The cells were carefully washed with PBS and then lysed using a lysis buffer (0.1 wt

(13) Gelatin Meshes Enriched with Graphene Oxide and Magnetic Nanoparticles Support and Enhance the Proliferation and Neuronal Differentiation of Human Adipose-Derived Stem CellsInternational Journal of Molecular SciencesDecember 29, 2022Aida Șelaru, Alexandra-Elena Mocanu-Dobranici, Elena Olăreț, Raluca-Elena Ginghină, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescuof the scaffold was assessed using the “In vitro toxicology assay kit lactate dehydrogenase based” TOX7 Kit (Sigma Aldrich Co., Steinheim, Germany). The positive control for this assay was represented by

(14) A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatiteMaterials Today BioDecember 1, 2022Sarah-Sophia D. Carter, Abdul-Raouf Atif, Anna Diez-Escudero, Maja Grape, Maria-Pau Ginebra, Maria Tenje, Gemma Mestresproliferation was evaluated using the lactate dehydrogenase (LDH) biochemical assay (Sigma-Aldrich, ref. nr. TOX7-1 KT). This assay can be used as an indirect method to quantify the cytosolic enzyme LDH of cells that

(15) N-Hydroxyethyl acrylamide as a functional eROP initiator for the preparation of nanoparticles under â€œgreenerâ€ reaction conditionsâ€ Polymer ChemistryNovember 1, 2022Joachim C. Lentz, Robert Cavanagh, Cara Moloney, Bruno Falcone Pin, Kristoffer Kortsen, Harriet R. Fowler, Philippa L. Jacob, Eduards Krumins, Charlotte Clark, Fabricio Machado, et al.(Sigma-Aldrich), and at 37 °C with 5% CO2. The lactate dehydrogenase (LDH) release assay (Sigma Aldrich, TOX7 kit) and PrestoBlue cell viability assay (Thermo Fisher Scientific) were performed to assess cytotoxicity.

(16) The Transcriptional Regulation of Genes Involved in the Immune Innate Response of Keratinocytes Co-Cultured with Trichophyton rubrum Reveals Important Roles of Cytokine GM-CSFJournal of FungiOctober 31, 2022Monise Fazolin Petrucelli, Bruna Aline M. Cantelli, Mozart Marins, Ana Lúcia Fachinaliquot of cell supernatant of each experimental condition was mixed in a 96-well plate with 100 µL of TOX7 reagent (Sigma-Aldrich) according to the manufacturer’s instructions. Absorbance was read on a Multiscan

(17) Development of Biocomposite Alginate-Cuttlebone-Gelatin 3D Printing Inks Designed for Scaffolds with Bone Regeneration PotentialMarine DrugsOctober 26, 2022Filis Curti, Andrada Serafim, Elena Olaret, Sorina Dinescu, Iuliana Samoila, Bogdan Stefan Vasile, Horia Iovu, Adriana Lungu, Izabela Cristina Stancu, Rodica Marinescuquantified the cytotoxic effect of the scaffolds exerted on the cells. The test was performed using TOX7 kit (Sigma-Aldrich Co, Steinheim, Germany), according to the protocol indicated by the manufacturer,

(18) Identification and Utilization of a Chemical Probe to Interrogate the Roles of PIKfyve in the Lifecycle of β-CoronavirusesJournal of medicinal chemistryOctober 13, 2022Drewry DH, Potjewyd FM, Bayati A, Smith JL, Dickmander RJ, Howell S, Taft-Benz S, Min SM, Hossain MA, Heise M, et al.transferred to a clean plate. The clarified supernatant was stored frozen until analysis using the Sigma Tox7 kit according to the manufacturer’s protocol. Data was analyzed using GraphPad Prism to generate the

(19) Pulsed Laser Photo-Crosslinking of Gelatin Methacryloyl Hydrogels for the Controlled Delivery of Chlorpromazine to Combat Antimicrobial ResistancePharmaceuticsOctober 6, 2022Tatiana Tozar, Simona Nistorescu, Mihai Boni, Gratiela Gradisteanu Pircalabioru, Irina Negut, Angela Staicu(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma–Aldrich Co, Steinheim, Germany) and LDH (lactate dehydrogenase, Tox7 kit, Sigma–Aldrich) assays. MTT assay is a quantitative method used to evaluate cell-metabolic activity.

(20) Trabecular Titanium for Orthopedic Applications: Balancing Antimicrobial with Osteoconductive Properties by Varying Silver ContentsACS applied materials & interfacesSeptember 21, 2022Diez-Escudero A, Carlsson E, Andersson B, Järhult JD, Hailer NP.Darmstadt, Germany) supplemented to the complete media. A lactate dehydrogenase enzymatic assay (LDH, TOX7, Merck, KGaA, Darmstadt, Germany) was used to quantify cell adhesion and proliferation at 1, 7, 14,

Be the First to Write a Review!

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based from MilliporeSigma

MilliporeSigma

Citations (177)

Reviews

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based from MilliporeSigma

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map