Senescence Cells Histochemical Staining Kit CS0030 from MilliporeSigma

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ItemSenescence Cells Histochemical Staining Kit
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberCS0030
Quantity1KT

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) PURPL and NEAT1 Long Non-Coding RNAs Are Modulated in Vascular Smooth Muscle Cell Replicative SenescenceBiomedicinesDecember 6, 2023Clara Rossi, Marco Venturin, Jakub Gubala, Angelisa Frasca, Alberto Corsini, Cristina Battaglia, Stefano Bellostasenescence-associated-β-galactosidase (SA-β-gal) was evaluated by staining VSMCs with the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich, St. Louis, MO, USA), following the manufacturer’s instructions [13]. 2.3. Cell Proliferation VSMCs

(2) Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent agingEMBO reportsDecember 6, 2023Monami Ogura, Tatsuya Kaminishi, Takayuki Shima, Miku Torigata, Nao Bekku, Keisuke Tabata, Satoshi Minami, Kohei Nishino, Akiko Nezu, Maho Hamasaki, et al.staining SA‐β‐Gal staining was performed using the Senescence Cells Histochemical Staining Kit (Sigma, CS0030). Briefly, senescence‐induced cells cultured on collagen‐coated coverslips were fixed with Fixation

(3) Therapy-induced normal tissue damage promotes breast cancer metastasisiScienceNovember 22, 2023Douglas W. Perkins, Ivana Steiner, Syed Haider, David Robertson, Richard Buus, Lynda O'Leary, Clare M. Isackesolution in PBS. Slides were washed three times with PBS and incubated with X-gal staining mixture (Sigma CS0030) at 37°C without CO2 overnight. Next day, the slides were washed in PBS and counterstained with hematoxylin

(4) Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: In vitro‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profilingOncology ReportsNovember 15, 2023Lukáš Urban, Štepán Novák, Matúš Čoma, Barbora Dvořánková, Lukáš Lacina, Jana Šáchová, Miluše Hradilová, Petra Svatoňová, Michal Kolář, Hynek Strnad, et al.senescence-associated enzyme β-galactosidase (SAβGal) using a Senescence Cells Histochemical Staining kit (cat. no. CS0030; Sigma-Aldrich; Merck KGaA) according to the manufacturer's instructions. β-Galactosidase activity is

(5) Doxorubicin-Induced Cardiac Senescence Is Alleviated Following Treatment with Combined Polyphenols and Micronutrients through Enhancement in MitophagyCellsNovember 10, 2023Eleonora Foglio, Erica D’Avorio, Laura Vitiello, Laura Masuelli, Roberto Bei, Francesca Pacifici, David Della-Morte, Simone Mirabilii, Maria Rosaria Ricciardi, Agostino Tafuri, et al.investigated. Staining for SA-β-gal was performed with a Senescence Cell Histochemical Staining kit (CS0030; Sigma-Aldrich, MERCK, St. Louis, MO, USA), according to the manufacturer’s protocols. Briefly, 2 ×

(6) FGF21 alleviates adipose stem cell senescence via CD90 glycosylation-dependent glucose influx in remodeling healthy white adipose tissueRedox BiologyNovember 1, 2023Zixin Zhou, Huiying Zhang, Yan Tao, Jinhao Zang, Jingyuan Zhao, Huijie Li, Yalin Wang, Tianci Wang, Hui Zhao, Fuwu Wang, et al.FGF21 treatment. 2.5 SAβgal activity staining SAβgal activity was assayed using SAβgal staining kit (cs0030, Sigma-Aldrich; or C0602, Beyotime) based on the manufacturer's instruction. After fixation in fixing

(7) A Novel Role of Hyaluronic Acid and Proteoglycan Link Protein 1 (HAPLN1) in Delaying Vascular Endothelial Cell SenescenceBiomolecules & TherapeuticsNovember 1, 2023Dan Zhou, Ji Min Jang, Goowon Yang, Hae Chan Ha, Zhicheng Fu, Dae Kyong Kimremoving the medium, the cells were stained using the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich, St Louis, MO, USA) to detect the SA-β-gal activity in senescent cells, according to

(8) Cigarette smoke extract induces the senescence of endothelial progenitor cells by upregulating p300Tobacco Induced DiseasesOctober 3, 2023Guibin Liang, Zhihui He, Huaihuai Peng, Menghao Zeng, Xuefeng Zhangfinal test data. β-Gal staining Cells were stained with cell senescence detection kit (Millipore, CS0030, Billerica, MA, USA) for SA-gal activity. Then quantitative positive staining was performed with ImageJ. Western blot analysis

(9) Longevity-Associated Transcription Factor ATF7 Promotes Healthspan by Suppressing Cellular Senescence and Systematic InflammationAging and diseaseAugust 1, 2023Yaqun Huang, Ming-Xia Ge, Yu-Hong Li, Jing-Lin Li, Qin Yu, Fu-Hui Xiao, Hong-Shun Ao, Li-Qin Yang, Ji Li, Yonghan He, et al.(SA-β-gal) staining SA-β-gal staining was performed using a Senescence Cells Histochemical Staining Kit (CS0030-1kt, Sigma-Aldrich, USA) according to the manufacturer’s instructions. Briefly, cells were washed with

(10) Senescence-Driven Inflammatory and Trophic Microenvironment Imprints Mesenchymal Stromal/Stem Cells in Osteoarthritic PatientsBiomedicinesJuly 14, 2023Giuseppe Fusi, Michael Constantinides, Christina Fissoun, Lydiane Pichard, Yves-Marie Pers, Rosanna Ferreira-Lopez, Veronique Pantesco, Christophe Poulet, Olivier Malaise, Dominique De Seny, et al.(SA-β-Gal)-positive cells, the Senescence Cells Histochemical Staining Kit (Sigma-Aldrich, St. Louis, MI, USA; Cat#CS0030) was used according to the manufacturer’s instructions. Specifically, after 14 days of treatment, 12

(11) Induction of Senescence by Loss of Gata4 in Cardiac FibroblastsCellsJune 17, 2023Zhang Z, Shayani G, Xu Y, Kim A, Hong Y, Feng H, Zhu H.prepared as described above and stained using a SA β-gal staining kit (Sigma, Setagaya city, Japan, cat# CS0030-1KT) per the manufacturer’s protocol. Briefly, frozen heart sections were fixed with 4% PFA for 10 min

(12) hCINAP alleviates senescence by regulating MDM2 via p14ARF and the HDAC1/CoREST complexJournal of Molecular Cell BiologyJune 13, 2023Xinping Huang, Yan Zhao, Min Wei, Ruipeng Zhuge, Xiaofeng Zhengprocess, the staining mixture was prepared according to the manufacturer's instructions (Sigma Aldrich, CS0030). Cells were washed three times with 1 ml PBS/well and incubated overnight with the staining mixture

(13) A rationally designed fluorescence probe achieves highly specific and long‐term detection of senescence in vitro and in vivoAging CellJune 13, 2023Li Hu, Chanjuan Dong, Zhe Wang, Shengyuan He, Yiwen Yang, Meiting Zi, Huiqin Li, Yanghuan Zhang, Chuanjie Chen, Runzi Zheng, et al.staining Cell SA‐β‐Gal staining was performed using a Senescence Cells Histochemical Staining Kit (CS0030, Sigma‐Aldrich) according to the manufacturer's instructions. Briefly, cells were washed with PBS and

(14) Involvement of ACACA (acetyl-CoA carboxylase α) in the lung pre-metastatic niche formation in breast cancer by senescence phenotypic conversion in fibroblastsCell Oncol (Dordr)June 1, 2023Yung-Chi Huang, Ming-Feng Hou, Ying-Ming Tsai, Yi-Chung Pan, Pei-Hsun Tsai, Yi-Shiuan Lin, Chao-Yuan Chang, Eing-Mei Tsai, Ya-Ling HsuJ.T.Baker) and stained by 500 μL solution (pH 6.0) from a Senescence Cells Histochemical Staining Kit (CS0030-1KT, Sigma) for 16 h incubation at a CO2-free, 37℃ condition. Each experiment was performed three times

(15) Progesterone and its metabolite allopregnanolone promote invasion of human glioblastoma cells through metalloproteinase‑9 and cSrc kinaseOncology lettersJune 1, 2023Bello-Alvarez C, Zamora-Sánchez CJ, Peña-Gutiérrez KM, Camacho-Arroyo I.were washed with PBS and senescence was evaluated using the Senescent Cells Staining Kit (cat. no. CS0030-1KT; MilliporeSigma), according to the manufacturer's instructions, to determine the expression of β-galactosidase

(16) Lost in HELLS: Disentangling the mystery of SALNR existence in senescence cellular modelsPLOS ONEMay 30, 2023Arianna Consiglio, Marco Venturin, Sabrina Briguglio, Clara Rossi, Giorgio Grillo, Stefano Bellosta, Maria Grazia Cattaneo, Flavio Licciulli, Cristina Battagliachanges and by β-galactosidase (β-gal) staining with the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich). After reaching the preferred condition, cells were collected in TRIzol reagent (15596018,

(17) Effect of Monocerin, a Fungal Secondary Metabolite, on Endothelial CellsToxins (Basel)May 18, 2023Tainah Colombo Gomes, Rafael Conrado, Rodrigo Cardoso de de Oliveira, Priscila Jane Romano Gonçalves Selari, Itamar Soares de de Melo, Welington Luiz Araújo, Durvanei Augusto Maria, Ana Olívia De Souzathe senescence cell histochemical staining kit, according to the manufacturer’s instructions (Sigma #CS0030). Briefly, HUVECs at 1 × 105 cells/well in a 12-well plate were treated with monocerin at 0.02, 0.15,

(18) Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cellsCell death & diseaseMay 13, 2023Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G, et al.green,Invitrogen,C2925 11,DAPI,Invitrogen,P36966 12,Phalloidin 594,Invitrogen,A12381 ,Kits,Kits,Kits 1,Senescence Cells Histochemical Staining Kit,Sigma,CS0030 2,Mitochondria Isolation Kit,Sigma,MITOISO2-1KT 3,ELISA MAX™ Deluxe Set Mouse IL-4,BioLegend,431104 4,ELISA

(19) Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1PeerJApril 19, 2023Zijia Wang, Ying Han, Ying Peng, Shuhui Shao, Huanquan Nie, Kun Xia, Haofeng Xiong, Tong Sugalactosidase (SA-β-gal) activity assay The SA-β-gal activity was performed using a SA-β-gal staining Kit (CS0030; Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions. Briefly, after senescence

(20) TGF-β in the microenvironment induces a physiologically occurring immune-suppressive senescent stateCell reportsMarch 28, 2023Matsuda S, Revandkar A, Dubash TD, Ravi A, Wittner BS, Lin M, Morris R, Burr R, Guo H, Seeger K, et al.ß-galactosidase staining was performed using the Senescence Cells Histochemical Staining Kit (Cat # CS0030; Sigma-Aldrich). Briefly, cells were fixed and incubated with freshly prepared staining solution overnight

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Senescence Cells Histochemical Staining Kit from MilliporeSigma

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Senescence Cells Histochemical Staining Kit from MilliporeSigma

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