Signal Seeker Ubiquitin Enrichment Kit (30 assays) BK161 from Cytoskeleton, Inc.

Product Specs
ItemSignal Seeker Ubiquitin Enrichment Kit (30 assays)
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBK161
Detection Targetubiquitinated proteins
Applicationsubiquitinated protein detection (IP)
Reactivitywide range of species
QuantityKIT 30 assays

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Unfolded protein response inhibits KAT2B/MLKL-mediated necroptosis of hepatocytes by promoting BMI1 level to ubiquitinate KAT2BOpen Med (Wars)June 16, 2023Xiaogang Huang, Xiongzhi He, Rongxian Qiu, Xuemei Xie, Fengfeng Zheng, Feihua Chen, Zhenting HuUbiquitination assay The ubiquitination level of KAT2B was determined by Ubiquitination detection Kit (BK161; Cytoskeleton, USA) as previously described [17]. In short, cells transfected with shNC or shBMI1 were

(2) MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradationCell DiscoveryFebruary 21, 2023Yuan Wang, Zhenchang Jia, Chenxi Liang, Yunfei He, Min Cong, Qiuyao Wu, Pu Tian, Dasa He, Xiang Miao, Beibei Sun, et al.and immunoblotted. In vivo monoubiquitination assays The Signal-Seeker Ubiquitin Enrichment Kit (BK161, Cytoskeleton) was used according to the manufacturer’s instructions to pull down ubiquitinated proteins.

(3) Activation of RhoC by regulatory ubiquitination is mediated by LNX1 and suppressed by LIS1Sci RepOctober 3, 2022Stanislav Kholmanskikh, Shawn Singh, M. Elizabeth Rossfrom whole tissue or cell lysates using a SignalSeeker Ubiquitination Detection kit (Cytoskeleton, BK161), as per manufacturer’s protocol. HA-tagged and interacting proteins were pulled down from cell lysates

(4) Simvastatin modulates estrogen signaling in uterine leiomyoma via regulating receptor palmitoylation, trafficking and degradationPharmacological ResearchOctober 1, 2021Sadia Afrin et al.simvastatin on ER-α ubiquitination was examined using the Signal-Seeker Ubiquitination Detection Kit (BK161, Cytoskeleton, Denver, CO) according to the manufacturer’s instructions. HuLM cells were grown to 70%

(5) Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1BMC BiologyDecember 1, 2020Chunming Gu, Zhao Yin, Hong Nie, Yanjun Liu, Juhua Yang, Guiping Huang, Jianping Shen, Liguo Chen, Jia FeiSignal-Seeker™ Ubiquitination Detection Kit according to the protocol provided by the manufacturer (cat. #BK161, Cytoskeleton). Briefly, the appropriate amount of Ub (cat. #UBA01, Cytoskeleton) or control beads (Cat.

(6) The Na⁺ pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalizationJournal of cell scienceAugust 21, 2020Sen A, Hsieh WC, Hanna CB, Hsu CC, Pearson M 2nd, Tao WA, Aguilar RC.as mono-ubiquitylated products) included in the Signal-Seeker™ ubiquitylation detection kit (Cytoskeleton Cat. #BK161) following the manufacturer’s instructions. The Ena1-GFP from samples and 5% input lysates were resolved

(7) The Ubiquitin-Proteasome System Does Not Regulate the Degradation of Porcine β-Microseminoprotein during Sperm CapacitationInternational Journal of Molecular SciencesJune 10, 2020Lucie Tumova, Michal Zigo, Peter Sutovsky, Marketa Sedmikova, Pavla PostlerovaAffinity Isolation of Polyubiquitinated Proteins Signal-SeekerTM Ubiquitination Detection kit (cat# BK161, Cytoskeleton, Denver, CO, USA) was used to isolate ubiquitinated proteins from spermatozoa according

(8) Mechanical regulation of glycolysis via cytoskeleton architectureNatureFebruary 1, 2020Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, et al.pull-down. Ubiquitination of PFKP was detected using a Signal-Seeker Ubiquitination Detection Kit (BK161, Cytoskeleton, Denver, CO) according to the manufacturer’s instruction. In summary, HEK cells were washed

(9) O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma ProliferationTheranosticsJanuary 1, 2020Chu Y, Jiang M, Wu N, Xu B, Li W, Liu H, Su S, Shi Y, Liu H, Gao X, et al.were boiled for further western blotting. Ubiquitination test An ubiquitination detection Kit (Cat# BK161, Cytoskeleton, USA) was used according to the assay protocol. Cells were cultured in 150 cm2 plates

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Signal Seeker Ubiquitin Enrichment Kit (30 assays) from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Signal Seeker Ubiquitin Enrichment Kit (30 assays) from Cytoskeleton, Inc.

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