Fig 1: (A) Glutamate concentration, (B) glutaminase function, and (C) VGLUT2 levels. The absolute control value of VGLUT2 protein levels was 26.31 ± 0.87 ng/g. Results show the mean (n = 9) ± SEM of three independent experimental conditions from cells of different cultures, each developed in triplicate. There were no significant differences between controls treated with and without a vehicle, cells transfected without siRNA or with scramble siRNA, or cells transfected with or without siRNA and treated with a vehicle in any of the targets studied. The data shown in the figures represent control treated with vehicle and transfected without siRNA as 100%. One-way (treatment-response comparisons) and two-way (transfection/treatment comparisons) ANOVA analyses followed by Tukey post-hoc test were developed to determine statistically significant differences between treatments. *** p = 0.001, significantly different from controls; &&& p = 0.001 contrasted with BPA exposure.