Fig 1: uMSC-CM inhibits the progression of radiation ulcer in rat models. (a) Representative images of hind limbs from irradiated rats and uMSC-CM treatment rats. (b) ELISA analysis the serum level of IL-1a in different group rats. (c) ELISA analysis the serum level of ANG-1 in different group rats. (d) Histological analysis of skin tissues from irradiated rats and uMSC-CM treatment rats. (e) Representative immunofluorescence pictures of p16 in skin sections from irradiated rats and uMSC-CM treatment rats. (f) Western blot analysis of p16, p21 and p53 expression in different group rats. Data are presented as mean ± SD, n = 3, two-way analysis of variance with Bonferroni’s post hoc comparison; *p < 0.05, **p < 0.01. Ctrl control, IR ionizing radiation, ns no significance, uMSC-CM conditioned medium derived from human umbilical cord mesenchymal stem cells, IL-1a interleukin-1a, ANG-1 angiopoeitin-1
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