Fig 1: Reverse transcription-quantitative PCR analysis of CFTR and ß-actin expression. *P<0.01. Groups: A, =30 years; B, =31 and =35 years; and C, =36 years. CFTR, cystic fibrosis transmembrane conductance regulator.
Fig 2: Dot plot of the correlation between CFTR and estradiol levels among the three groups. CFTR, cystic fibrosis transmembrane conductance regulator; E2, estradiol.
Fig 3: Immunofluorescence staining of CCs for CFTR in (A) group A, (B) group B and (C) group C. On the cell culture dish with CCs, CFTR (red) was readily detected in the cytoplasm. The nuclei were stained with DAPI (blue) (magnification, x400; scale bar, 50 µm). (D) Fluorescence intensity histogram for the 3 groups. Groups: A, =30 years; B, =31 and =35 years; and C, =36 years. CCs, cumulus cells; CFTR, cystic fibrosis transmembrane conductance regulator.
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