Fig 1: NaB inhibited LRP3 inflammasome pathway in db/db mice.Epididymal adipose tissues (EAT) and subcutaneous adipose tissue (SAT) were harvested from db/db mice treated with or without NaB for six weeks. Total RNA was isolated for realtime RT-PCR (A). Data are expressed in ?Ct values normalized against the mean Ct of GAPDH. Fold changes were calculated as 2^(??CtN-NaB). Total proteins were isolated for Western-blot (B). The bar graph of the densitometry measurement showed the decreased expression of NLRP3 and active IL-1 in db/db adipose tissues treated without or with NaB (C). *P < 0.05, #P < 0.01 by Student’s t test.
Fig 2: HDACi inhibited the cytokine expression in 3T3-L1 adipocytes.3T3-L1 cells were treated with TNF-a (50 ng/mL) combined with or without NaB(1 mM) or TSA(0.5 µM) for 8 hr. Total RNA was isolated for realtime RT-PCR to detect the gene expression of IL-1ß and IL-6 (A). Supernatants of cell culture were harvested for ELISA assay to detect IL-1 and IL-6 in the culture medium (B). N: Normal control. *P < 0.05 by t-test (TNF vs NaB or TSA).
Fig 3: HDACi inhibited NLRP3 inflammasome pathway in 3T3-L1 cells.3T3-L1 cells were treated with TNF-a combined without or with NaB (1 mM) and TSA (0.5 µM) for 8 hr. (A) Total RNA was isolated for realtime RT-PCR. mRNA expression was normalized to that of GAPDH. (B) Total proteins were isolated from treated 3T3-L1 cells for Western blots to detect the protein level of NLRP3 and IL-1. GAPDH was used as controls for equal loading. N: Normal control. (C) Densitometry measurement of Western blots. The experiments were repeated at least three times. *P < 0.05, #P < 0.01 by t-test (TNF vs NaB or TSA).
Supplier Page from CUSABIO Technology LLC for Mouse Interleukin 1β,IL-1β ELISA Kit