Fig 1: A. muciniphila regulates the expression of immune factors. (A) The levels of MMP-1, MMP-9, CTSB, and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 µm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-?, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and a-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA (n = 10 mice/group). *Compared with the control group, P < 0.05; #compared with the AAA group, P < 0.05.
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