Fig 1: M1/M2-associated protein level changes after JWH133, GP1a or AM630 treatment. a-d M1-associated protein levels, including IL-6, IL-12, CD86 and iNOS. e-h M2-associated protein levels, including IL-4, IL-10, CD206 and Arg-1. i and j The iNOS/Arg-1 and IL-12/IL-10 protein level. Data are mean ± SD. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
Fig 2: FKN deficiency suppressed F4/80, iNOS and ARG-1 protein localization in mice kidney tissues via inhibition of Wnt/ß-catenin signalling. Scale bars represent 50 µm
Fig 3: FKN regulated polarization in LPS-induced J774A.1 cells via Wnt/ß-catenin signalling. A, B, Western blot analysis and their respective quantitation were performed to detect the expression levels of iNOS, TNF-a, ARG-1 and IL-10 protein in J774A.1 cells. C, D, ELISA was used to detect the levels of TNF-a and ARG-1 in the cell supernatants. * P < .05 compared with the control group; # P < .05 compared with the LPS group. E, F, Immunofluorescence analysis was used to ascertained the subcellular localization of iNOS and ARG-1. Scale bars represent 10 µm
Fig 4: FKN deficiency suppressed macrophages polarization and proliferation in mice kidney tissues via inhibition of Wnt/ß-catenin signalling. A, B, Western blot analysis and their respective quantitation showing the protein expression of FKN, ß-catenin, Wnt-4, c-myc, cyclinD1, iNOS, TNF-a, ARG-1 and IL-10 in mice kidney tissues. * P < .05 compared with the WT mice; # P < .05 compared with the LPS mice
Fig 5: M1/M2-associated gene expression changes after JWH133, GP1a or AM630 treatment. a-d M1-associated mRNA expressions, including IL-6, IL-12, CD86 and iNOS. e-h M2-associated mRNA expressions, including IL-4, IL-10, CD206 and Arg-1. Data are mean ± SD. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
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