Fig 1: The fold change alternations in mRNA levels. Bar diagram showing mRNA levels fold change of MCF-7 cells (I) and MCF-7 Xenograft mice (II) treated with single or combinatorial treatments relative to control. Bax, p53, caspase-3, caspase-9, Cytochrome C, and Bcl2 as apoptotic and anti-apoptotic genes (A), Beclin-1, ATG7, PI3K, and AKT as autophagic and growth proliferative signaling genes (B). Values are expressed as mean ± SE, n = 3. Data are presented as mean ± SE (error bar) of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with control (untreated -cells or -mice) groups.
Fig 2: The disparity of apoptotic, anti-apoptotic, autophagy markers. Bar diagrams showing apoptotic markers as caspase-3, caspase-8, P53 (A), Bcl2, KI-67 as proliferation markers (B), ATG4B (C) and ATG5 (D) as autophagic markers of MCF-7 cells (I) and MCF-7 Xenograft mice (II) treated with single or combinatorial treatments. The data are presented as mean ± SE of at least three independent experiments. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 compared with control (untreated group). + p < 0.05, ++ p < 0.01, +++ p < 0.001, ++++ p < 0.0001 compared with the DOX treated group.
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