Fig 1: M1/M2-associated protein level changes after JWH133, GP1a or AM630 treatment. a-d M1-associated protein levels, including IL-6, IL-12, CD86 and iNOS. e-h M2-associated protein levels, including IL-4, IL-10, CD206 and Arg-1. i and j The iNOS/Arg-1 and IL-12/IL-10 protein level. Data are mean ± SD. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
Fig 2: M1/M2-associated gene expression changes after JWH133, GP1a or AM630 treatment. a-d M1-associated mRNA expressions, including IL-6, IL-12, CD86 and iNOS. e-h M2-associated mRNA expressions, including IL-4, IL-10, CD206 and Arg-1. Data are mean ± SD. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
Fig 3: Macrophages infiltration alteration after JWH133, GP1a or AM630 treatment. a and b Representative immunofluorescence stains of M1 macrophages (CD86 green color; F4/80 red color; nuclei blue color) and M2 macrophages (CD206 green color; F4/80 red color; nuclei blue color) in the wound zone at 5 days post-injury. Scale bar = 50 μm. c and d Cell counting changes of M1 and M2 macrophage after JWH133, GP1a or AM630 treatment. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
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