Aurum™ Total RNA Mini Kit 7326820 from Bio-Rad

Product Specs
ItemAurum™ Total RNA Mini Kit
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7326820
CapacityBinding capacity: >100 µg purified total RNA
FormatMini column
PurityA₂₆₀/A₂₈₀ of 1.9–2.1
VolumeMaximum elution volume: 80 µl (2 elutions of 40 µl each)
Minimum elution volume: 30 µl*
Yield20 µg/40 mg brain
Assay Time30–60 min (including DNase I digest)
Brand/TrademarkLSG GXD
SourceCell/Tissue, Fungal, Plant

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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(1) Quinolinic acid impairs mitophagy promoting microglia senescence and poor healthspan in C. elegans: a mechanism of impaired aging processBiol DirectDecember 20, 2023Anjila Dongol, Xi Chen, Peng Zheng, Zehra Boz Seyhan, Xu-Feng HuangQuantitative real-time PCR (qRT-PCR) Total RNA was extracted using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, #7326820) and reversed to cDNA by using Applied Biosystems High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific

(2) Dietary Vitamin D Mitigates Coronavirus-Induced Lung Inflammation and Damage in MiceVirusesDecember 15, 2023Gabriel Campolina-Silva, Ana Cláudia Dos Santos Pereira Andrade, Manoela Couto, Paloma G. Bittencourt-Silva, Celso M. Queiroz-Junior, Larisse de Souza B. Lacerda, Ian de Meira Chaves, Leonardo C. de Oliveira, Fernanda Martins Marim, Cleida A. Oliveira, et al.(RT-qPCR) Total RNA was isolated from 20 mg of lung tissue using the AurumTM RNA Kit (Catalog No. 7326820, Bio-Rad, Hercules, CA, USA) and then reverse-transcribed into cDNA with the iScriptTM cDNA Kit (Catalog

(3) Pericytes are protective in experimental pneumococcal meningitis through regulating leukocyte infiltration and blood–brain barrier functionJournal of NeuroinflammationNovember 17, 2023Nina C. Teske, Susanne Dyckhoff-Shen, Paul Beckenbauer, Jan Philipp Bewersdorf, Joo-Yeon Engelen-Lee, Sven Hammerschmidt, Roland E. Kälin, Hans-Walter Pfister, Matthijs C. Brouwer, Matthias Klein, et al.brain sections as well as cultured pericytes was extracted using Aurum Total RNA Mini Kit (Bio-Rad, 7326820) according to manufacturer’s instructions. Reverse transcription into cDNA was achieved using the iScript cDNA Synthesis Kit

(4) Impaired mitophagosome–lysosome fusion mediates olanzapine‐induced agingAging CellNovember 1, 2023Xi Chen, Zhizhen Wang, Peng Zheng, Anjila Dongol, Yuanyi Xie, Xing Ge, Mingxuan Zheng, Xuemei Dang, Zehra Boz Seyhan, Nathan Nagaratnam, et al.was purchased from Invivogen. DC protein assay reagent kit (5000116) and Aurum™ Total RNA Mini Kit (7326820) were purchased from Bio‐Rad. Enhancing ATP Assay kit (S0027) was purchased from Beyotime. SensiFAST™

(5) Hepatoprotective and Antioxidant Effects of Nanopiperine against Cypermethrin via Mitigation of Oxidative Stress, Inflammations and Gene Expression Using qRT-PCRInternational Journal of Molecular SciencesOctober 19, 2023Sohail Hussain, Abdulmajeed M. Jali, Saeed Alshahrani, Khairat H. M. Khairat, Rahimullah Siddiqui, Mohammad Intakhab Alam, Raisuddin Ali, Manal Mohammed, Andleeb Khan, Hamad Al Shahi, et al.UV trans-illuminator. 4.13. Quantitative Real Time Polymerase Chain Reaction An RNA Mini Kit (Cat#7326820, BIO-RAD) was used to isolate RNA from the tissue. cDNA was synthesized from RNA using a High-Capacity

(6) Cnot3 is required for male germ cell development and spermatogonial stem cell maintenancebioRxivOctober 19, 2023Qing Chen, Safia Malki, Xiaojiang Xu, Brian Bennett, Brad L. Lackford, Oleksandr Kirsanov, Christopher B. Geyer, Guang HuBiosciences). ID4-EGFP+ cell pellets were centrifuged at 300g for 20 min and stored in RNA lysis buffer (Cat# 7326820, Bio-Rad, Aurum™ Total RNA Mini Kit) at −80°C until use. The cellular level of ROS and Glutathione

(7) RIT1 regulation of CNS lipids RIT1 deficiency Alters cerebral lipid metabolism and reduces white matter tract oligodendrocytes and conduction velocities☆HeliyonOctober 1, 2023Lei Wu, Fang Wang, Carole L. Moncman, Mritunjay Pandey, Harrison A. Clarke, Hilaree N. Frazier, Lyndsay E.A. Young, Matthew S. Gentry, Weikang Cai, Olivier Thibault, et al.isolated from pulverized brain tissues (n = 3 each group) by using Aurum Total RNA Mini Kit (BioRad, 7326820). 1 μg total RNA was reverse-transcribed using a Bio-Rad RT kit. The cDNA was diluted 1:5 in ultrapure

(8) Sigma-2 Receptor Ligand Binding Modulates Association between TSPO and TMEM97International journal of molecular sciencesMarch 28, 2023Thejer BM, Infantino V, Santarsiero A, Pappalardo I, Abatematteo FS, Teakel S, Van Oosterum A, Mach RH, Denora N, Lee BC, et al.µg/mL puromycin. 4.9. RT-PCR RNA extraction was performed using the Total RNA mini kit (Bio-Rad, #7326820, Melbourne, VIC, Australia) following the manufacturer’s instructions. RNA concentration was measured

(9) Neuroprotective Effects of Nano-Curcumin against Cypermethrin Associated Oxidative Stress and Up-Regulation of Apoptotic and Inflammatory Gene Expression in Rat BrainsAntioxidants (Basel)March 4, 2023Mohammad Ashafaq, Sohail Hussain, Saeed Alshahrani, Rahimullah Siddiqui, Mohammad Intakhab Alam, Manal Mohamed Elhassan Taha, Yosif Almoshari, Saad S. Alqahtani, Abdulmajeed M. Jali, Hashim M. AljohaniCPM, and NC treated groups was isolated from the brain cortex using the Aurum™ Total RNA Mini Kit (Cat#7326820, BIO-RAD). cDNA was synthesized using a High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

(10) Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathyNano TodayFebruary 1, 2023Weiang Yan, Alireza Rafieerad, Keshav Narayan Alagarsamy, Leena Regi Saleth, Rakesh C. Arora, Sanjiv Dhingrananosheets at 2 µg/mL for 48 h. Total cellular RNA was isolated using the Aurum™ Total RNA Mini Kit (7326820, Bio-Rad) and quantified using a NanoDrop™ Spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized

(11) Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT)Proceedings of the National Academy of SciencesJanuary 31, 2023Jeffrey Y. Zhou, Mrinal K. Sarkar, Ken Okamura, John E. Harris, Johann E. Gudjonsson, Katherine A. FitzgeraldSigma. RNA Isolation and qPCR. Total RNA was extracted using the Aurum™ Total RNA Mini Kit (Bio-Rad #7326820), and cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708890). Quantitative PCR

(12) Mutant p53 gain of function mediates cancer immune escape that is counteracted by APR-246British Journal of CancerNovember 23, 2022Xiaolei Zhou, Gema Sanz Santos, Yue Zhan, Mariana M. S. Oliveira, Shiva Rezaei, Madhurendra Singh, Sylvain Peuget, Lisa S. Westerberg, John Inge Johnsen, Galina Selivanovafrom total cell lysate and reverse transcribe RNA to cDNA, AurumTM Total RNA Mini Kit (Bio-Rad cat#7326820) and iScriptTM Reverse Transcription Supermix (Bio-Rad cat#1708841), respectively, was used according

(13) Ezh2Y641F mutations co-operate with Stat3 to regulate MHC class I antigen processing and alter the tumor immune response in melanomaOncogeneNovember 11, 2022Sarah M. Zimmerman, Samantha J. Nixon, Pei Yu Chen, Leela Raj, Sofia R. Smith, Rachel L. Paolini, Phyo Nay Nay Lin, George P. Souroullasanalysis. Quantitative PCR RNA was isolated from melanoma cells using the Aurum Total RNA Mini Kit (Bio-Rad Cat# 7326820) according to the manufacturer’s instructions. The igScript First Strand cDNA Synthesis Kit (Intact Genomics

(14) Characterizing and Targeting Genes Regulated by Transcription Factor MYBL2 in Lung Adenocarcinoma CellsCancersOctober 11, 2022Lee Y, Wu Z, Yang S, Schreiner SM, Gonzalez-Smith LD, Rhie SK.previously performed siRNA experiments [15]. RNA was isolated using the Aurum total RNA mini kit (Cat #7326820, Bio-Rad, Hercules, CA, USA), following the manufacturer’s manual. 2.8. RNA-Seq RNA was isolated from

(15) Ubiquitination as a key regulatory mechanism for O(3)-induced cutaneous redox inflammasome activationRedox biologyOctober 1, 2022Ferrara F, Cordone V, Pecorelli A, Benedusi M, Pambianchi E, Guiotto A, Vallese A, Cervellati F, Valacchi G.skin models, total RNA was extracted using the Aurum Total RNA Mini Kit with DNase digestion (Cat. 7326820, Bio-Rad Laboratories, Inc., Hercules, CA, USA), according to the manufacturer’s recommended procedure.

(16) Mast Cell Proteases Promote Diverse Effects on the Plasminogen Activation System and Wound Healing in A549 Alveolar Epithelial CellsCellsSeptember 18, 2022Mogren S, Berlin F, Eskilsson L, Van Der Burg N, Tufvesson E, Andersson CK.stimulation, mRNA was collected and isolated with Aurum total RNA MiniKit (Bio-Rad, Hercules, CA, USA, 7326820). RNA concentration was measured with nanodrop 2000c Thermo Scientific (Waltham, MA, USA). Using RT2

(17) Attenuation of Autism-like Behaviors by an Anthocyanin-Rich Extract from Portuguese Blueberries via Microbiota–Gut–Brain Axis Modulation in a Valproic Acid Mouse ModelInternational Journal of Molecular SciencesAugust 17, 2022Diana Serra et al.tissue samples by using the RNA extraction kit AurumTM Total RNA Mini (Bio-Rad, Hercules, CA, USA, 7326820,), according to the manufacturer’s instructions. Complementary DNA synthesis was performed using 1 µg

(18) Nucleotide- and Protein-Dependent Functions of Actg1Molecular Biology of the CellAugust 1, 2022Lauren J. Sundby, William M. Southern, Katelin M. Hawbaker, JesÃºs M. Trujillo, Benjamin J. Perrin, James M. ErvastiRNA was extracted from homogenized tissue and MEF samples using the Bio-Rad Aurum Total RNA Mini-Kit (7326820) according to manufacturer’s instructions. RNA concentration was measured using a NanoDrop 1000 spectrophotometer

(19) RAS-Driven Macropinocytosis of Albumin or Dextran Reveals Mutation-Specific Target Engagement of RAS p.G12C Inhibitor ARS-1620 by NIR-Fluorescence ImagingMolecular Imaging and BiologyJune 1, 2022Margie N. Sutton, Seth T. Gammon, Riccardo Muzzioli, Federica Pisaneschi, Bhasker Radaram, Ping Yang, David Piwnica-Worms6-well plate at 1.5×105 cells/well. RNA was extracted using the Aurum Total RNA Mini Kit from Bio-Rad (7326820). SYBR green-based quantitative PCR was used to measure mRNA levels (BioRad 1725150) (Primers 5’ → 3’:

(20) Novel CFTR modulator combinations maximise rescue of G85E and N1303K in rectal organoidsERJ Open ResearchApril 19, 2022Marjolein M. Ensinck, Liesbeth De Keersmaecker, Anabela S. Ramalho, Senne Cuyx, Stephanie Van Biervliet, Lieven Dupont, Frauke Christ, Zeger Debyser, François Vermeulen, Marianne S. Carlonby reverse transcriptase PCR (RT-PCR). For RT-PCR, RNA was isolated using Aurum Total RNA mini kit (#7326820; Bio-Rad, Hercules, CA, USA) and converted to cDNA with the High-Capacity cDNA Reverse Transcription

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(1)

11/16/2021Good RNA Isolation Kit Wei WeiThis is good DNA-free total RNA isolation kit from cells.
(1)

6/10/2020High Quality RNA Extraction Kit sndp kmrI strongly recommend this kit for RNA isolation followed by quantitative gene expression studies

Aurum™ Total RNA Mini Kit

Aurum™ Total RNA Mini Kit from Bio-Rad

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Bio-Rad

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Aurum™ Total RNA Mini Kit from Bio-Rad

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