Fig 1: Effect of SJT on pro-inflammatory cytokines in the serum of AIA rats. The levels of IL-6 (A), TNF-a (B), and IL-1ß (C) were determined by ELISA assay. Data were expressed as mean ± S.D. vs. model, *p < 0.05, **p < 0.01; vs. normal, # p < 0.05, ## p < 0.01.
Fig 2: The effects of latifolin on macrophage secretome in vitro. Latifolin reduces inflammatory cytokine (A) IL-6; (B) IL-1β; (C) TNF-α level. Data were shown as mean ± SD, n = 3. #P < 0.05 versus control, *P < 0.05 versus model group.
Fig 3: CK2 overexpression reduced cell inflammation, apoptosis and oxidative stress through changing NR2B phosphorylation level and NR2B-PSD95 complex. pc-NR2B was transfected into Hemin + pc-CK2 treated neurons and astrocytes. (A) Expression of NR2B, NR2B phosphorylation sites S1480, S1303, and Tyr1472 were determined in cells using Western blot. (B) Representative gel images and quantification of co-immunoprecipitation (co-IP) show the level of NR2B-PSD95 complex in cells. (C) Expressions of NR2B in cells were detected using RT-qPCR. (D) NR2B expression was detected using Western blot. (E) Levels of TNF-α and IL-6 in astrocytes were detected using ELISA. (F) Cell apoptosis was measured using TUNEL assay. (G) Content of ROS was determined by fluorescence probe DCFH-DA. The cell experiment was repeated three times independently. Data were expressed as mean ± standard deviation. **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 4: Overexpression of CK2 reduced cellular inflammation and alleviated oxidative stress. pc-CK2 was transfected into Hemin-treated neurons and astrocytes. (A) Levels of TNF-α and IL-6 in astrocytes were detected using ELISA. (B) Content of ROS was determined by fluorescence probe DCFH-DA. (C–E) Contents of GSH, SOD, and MDA were detected using the kits. The cell experiment was repeated three times independently. Data were expressed as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 5: The effect of GAL on DOX-induced changes inflammatory markers. (A) IL-1ß, (B) IL-6, and (C) TNF-a in the brain of rats. The data were analyzed using a one-way ANOVA with Dunnett’s analysis and were considered significant when * p < 0.05, *** p < 0.001, and **** p < 0.0001 as compared to the control/DOX group.
Supplier Page from ABclonal Technology for Rat IL-6 ELISA Kit