KAPA HiFi Kits from Roche

Product Specs
ItemKAPA HiFi Kits
CompanyRoche
Price Request Sample
Catalog NumberKapa Hifi
QuantityKit
ApplicationsAmplification of target materials and Screening libraries
Sample TypeDNA, cDNA, plasmid DNA, bisulfite-treated DNA

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Understanding the bacterial compositional network associations between oral and gut microbiome within healthy KoreansJournal of Oral MicrobiologyDecember 31, 2023Jinuk Jeong, Kung Ahn, Seyoung Mun, Kyeongeui Yun, Yeon-Tae Kim, Won Jung, Kyung Eun Lee, Moon-Young Kim, Yongju Ahn, Kyudong Hanregion of the bacterial 16S rRNA gene. PCR for amplifying the target region was performed using the KAPA HiFi Hot Start Ready Mix (2×) (Roche, Mannheim, Germany), and a pair of 16S V3-V4 target-specific primer

(2) Age-associated DNA methylation changes in Xenopus frogsEpigeneticsDecember 31, 2023Marco Morselli, Ronan Bennett, Nikko-Ideen Shaidani, Marko Horb, Leonid Peshkin, Matteo PellegriniResearch) according to the manufacturer’s instructions. The final amplification was performed with KAPA HiFi U+ (Roche Sequencing), IDT xGen Primers (20 µM – Integrated DNA Technologies), for a total of 12 PCR

(3) An efficient, scarless, selection-free technology for phage engineeringRNA BiologyDecember 31, 2023Moran G. Goren, Tridib Mahata, Udi QimronSigma-Aldrich, Agar was from Hylabs. D-maltose, MgSO4, CaCl2, Kanamycin and M-Toluic acid were from Merck. KAPA HiFi HotStart Ready Mix was from Kapa Biosystems. PCRBIO HS Taq DNA polymerase was from PCR Biosystems. NucleoSpin

(4) In vitro faecal fermentation of Tritordeum breads and its effect on the human gut healthCurr Res Microb SciDecember 20, 2023Kashika Arora, Giulia Gaudioso, Pavel Solovyev, Kieran Tuohy, Raffaella Di Cagno, Marco Gobbetti, Francesca Favawith a reaction volume of 25 µL. The reaction mix per sample consisted of 9 µL PCR H2O, 12.5 µL 2 × KAPA Hifi HotStart Ready Mix (Kapa Biosystems Ltd., UK), 0.5 μL of each primer (1 M) and 2.5 μL DNA (5 ng/μL).

(5) Rapid modeling of an ultra-rare epilepsy variant in wild-type mice by in utero prime editingbioRxivDecember 19, 2023Colin D. Robertson, Patrick Davis, Ryan R. Richardson, Philip H. Iffland, Daiana C. O. Vieira, Marilyn Steyert, Paige N. McKeon, Andrea J. Romanowski, Garrett Crutcher, Eldin Jašarević, et al.hU6 promoter. The vectors containing pegRNA and PE3/PE3b guides were used as template for PCR using KAPA HiFi HotStart DNA Polymerase with 2x Master Mix (Roche) to amplify parts containing the U6 promoter and either

(6) Impact of milk secretor status on the fecal metabolome and microbiota of breastfed infantsGut MicrobesDecember 18, 2023Aidong Wang, Aly Diana, Sofa Rahmannia, Rosalind S Gibson, Lisa A Houghton, Carolyn M Slupskyreaction volumes containing 4 μL DNA template, 0.75 μL DMSO (Fisher Scientific, Waltham, MA), 3 μL 5X KAPA HiFi Buffer (KAPA Biosystems, Woburn, MA), 0.45 μL dNTP Mix (10 mM), 0.3 μL KAPA HiFi HotStart Polymerase

(7) Genomic hypomethylation in cell-free DNA predicts responses to checkpoint blockade in lung and breast cancerSci RepDecember 18, 2023Kyeonghui Kim, Hyemin Kim, Inkyung Shin, Seung-Jae Noh, Jeong Yeon Kim, Koung Jin Suh, Yoo-Na Kim, Jung-Yun Lee, Dae-Yeon Cho, Se Hyun Kim, et al.using the Agencourt AMPure XP system (Beckman Coulter). Finally, the library was amplified using the KAPA HiFi HotStart polymerase (KAPA Biosystems) with Illumina’s P5 and P7 sequencing primers in the following

(8) The CDK12 inhibitor SR-4835 functions as a molecular glue that promotes cyclin K degradation in melanomaCell Death DiscovDecember 16, 2023Thibault Houles, Jonathan Boucher, Geneviève Lavoie, Graham MacLeod, Sichun Lin, Stephane Angers, Philippe P. Rouxmanufacturer’s protocol. 50 μg of gDNA from each sample was amplified in 20 individual PCR reactions using Kapa HiFi Master Mix (Kapa Biosystems) using TKOv3 library primers (forward: 5’-GAGGGCCTATTTCCCATGATTC-3’, reverse:

(9) Genomic Characterization of the Mycoparasite Pestalotiopsis sp. Strain cr013 from Cronartium ribicolaPol J MicrobiolDecember 16, 2023Jinde Yu, Lei Kong, Shichang Fan, Mingjiao Li, Jing LiThe ends of selected fragments were ligated with T-tailed adapters and magnified over eight cycles by KAPA HiFi Hot-Start ReadyMix (Kapa Biosystems, Inc., USA). Then, the amplification products were subjected to

(10) Noninvasive sampling of the small intestinal chyme for microbiome, metabolome and antimicrobial resistance genes in dogs, a proof of conceptAnim MicrobiomeDecember 16, 2023Julie Menard, Sahar Bagheri, Sharanya Menon, Y. Tina Yu, Laura B. Goodmanregion was amplified using the 515F and 806R for the V4 region primers in a 35 cycle PCR using the KAPA HiFi HotStart master mix (Roche Sequencing). A maximum volume of 13.5 µl of template DNA was used for all

(11) Combining the Tyrosine Kinase Inhibitor Cabozantinib and the mTORC1/2 Inhibitor Sapanisertib Blocks ERK Pathway Activity and Suppresses Tumor Growth in Renal Cell CarcinomaCancer ResDecember 15, 2023Yige Wu, Siqi Chen, Xiaolu Yang, Kazuhito Sato, Preet Lal, Yuefan Wang, Andrew T. Shinkle, Michael C. Wendl, Tina M. Primeau, Yanyan Zhao, et al.fragments were subsequently eluted from the capture reagent. The enriched libraries were amplified with the KAPA HiFi master mix (Roche). To quantify the concentration of each captured library pool accurately, qPCR was

(12) Relationships of Late Pleistocene giant deer as revealed by Sinomegaceros mitogenomes from East AsiaiScienceDecember 15, 2023Bo Xiao, Alba Rey-lglesia, Junxia Yuan, Jiaming Hu, Shiwen Song, Yamei Hou, Xi Chen, Mietje Germonpré, Lei Bao, Siren Wang, et al.hybridization for 30 h at 55 °C at all relevant steps. Post-capture amplification was performed using KAPA HiFi uracil+ premix (KAPA Biosystems) for 14 cycles following MYbaits v.3.0 protocol set up recommendations.

(13) CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cellsiScienceDecember 15, 2023Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, et al.FC-131-2002, Set-C: FC-131-2003, or Set-D: FC-131-2004) were added to the amplicons by PCR using polymerase KAPA HiFi Hot-Start Ready-Mix (Roche #0795893500). Program: [95°C_3min] x 1, [95°C_30s | 55°C_30s | 72°C_30s]

(14) Characterization and AAV-mediated CRB gene augmentation in human-derived CRB1KO and CRB1KOCRB2+/− retinal organoidsMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Nanda Boon, Xuefei Lu, Charlotte A. Andriessen, Michaela Orlovà, Peter M.J. Quinn, Camiel J.F. Boon, Jan Wijnholdspairs with unique sequence combinations for demultiplexing and sample identification (Table S3) using Kapa HiFi 2x Ready Mix (Roche; KK2602) and subsequently the purification using AMPure XP kit was carried out.

(15) Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribineClinical and Experimental ImmunologyDecember 13, 2023Elina Järvinen, Fumi Suomi, James B Stewart, Dimitri Guala, Miko Valori, Lilja Jansson, Janne Nieminen, Thomas G McWilliams, Pentti J Tienariwere used to amplify 9.1 kb and 11.1 kb overlapping amplicons of the mitochondrial genome using the Kapa HiFi long-range polymerase (KM3005 Roche). The 9.1 kb fragment was amplified from the ex vivo cell DNA by

(16) 5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expressionNat CommunDecember 12, 2023Zheng Zhao, Liang Li, Ruichen Zeng, Liangguan Lin, Dongwei Yuan, Yejie Wen, Na Li, Yingying Cui, Shiming Zhu, Zhi-Min Zhang, et al.one-twentieth of the elution product was used as the template for PCR amplification with 35 cycles using a KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa, KK2801). For each sample, the BSP products of multiple DMRs

(17) Improving the RNA velocity approach with single-cell RNA lifecycle (nascent, mature and degrading RNAs) sequencing technologiesNucleic Acids ResearchDecember 11, 2023Chen Zhang, Yitong Fang, Weitian Chen, Zhichao Chen, Ying Zhang, Yeming Xie, Wenfang Chen, Zhe Xie, Mei Guo, Juan Wang, et al.removed. The beads containing cDNA were suspended in 200 μl of PCR reaction mix, comprised of 100 μl of KAPA HiFi HotStart 2x ReadyMix (Kapa Biosystems), 10 μl of PCR primer 1 (5′-GCTGTACCGATGCTAGGCTGTTCGTCACCATAGTTGCGTCTCATG-3′),

(18) ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cellsFront Cell Dev BiolDecember 8, 2023Ryo Niwa, Kouji Sakai, Mandy Siu Yu Lung, Tomoko Matsumoto, Ryuta Mikawa, Shotaro Maehana, Masato Suzuki, Yuki Yamamoto, Thomas L. Maurissen, Ai Hirabayashi, et al.instructions, and purified DNA was resuspended in 100 µL of water. Target sequences were amplified using KAPA HiFi HS ReadyMix (Kapa Biosystems, Cat. No. KK2602) polymerase chain reaction (PCR), enzymatic PCR product

(19) Revisiting the outcome of adult wild-type Htt inactivation in the context of HTT-lowering strategies for Huntington's diseaseBrain CommunDecember 7, 2023Sara Regio, Gabriel Vachey, Enrique Goñi, Fabio Duarte, Margareta Rybarikova, Mélanie Sipion, Maria Rey, Maite Huarte, Nicole Déglonthen adjusted to 1 ng/μL. TIDE analysis We used 100 ng gDNA for an amplification reaction with the KAPA HiFi Hotstart kit (KAPA Biosystems, Labgene, Chatel-Saint-Denis, Switzerland). The reaction mixture contained

(20) Dietary supplementation with 1-kestose induces altered locomotor activity and increased striatal dopamine levels with a change in gut microbiota in male micePhysiol RepDecember 6, 2023Altanzul Altaisaikhan, Kazufumi Yoshihara, Tomokazu Hata, Noriyuki Miyata, Yasunari Asano, Takafumi Suematsu, Yoshihiro Kadota, Nobuyuki Sudopurified using NucleoFast 96 (U3100B; Takara Bio, Shiga, Japan). A second PCR was conducted using the KAPA Hifi Hotstart Ready Mix (KK2602; Kapa Biosystems, Wilmington, MA, USA) to attach a unique combination of

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Kapa HiFi Kits

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