Fig 1: M6PR and EphB4 are enriched in exosomes from SRGN-overexpressing ESCC cells. (A) GO analysis of differentially expressed proteins in exosomes from KYSE150-SRGN. (B) PANTHER™ Pathway enrichment analysis for differentially expressed proteins in exosomes from KYSE150-SRGN. All enriched pathways were shown. (C) Western blot validation of the upregulated proteins identified by LC-MS/MS in SRGN Exo compared with Con Exo. The concentration of exosomes was measured by Nanosight NS500 and equal numbers of exosomes were used for Western blot analysis. (D) Western blot analysis of M6PR, EphB4, ALIX and CD63 expressions in density gradient fractions of exosomes from ESCC cells. After flotation of crude exosomes in iodixanol gradients, equal volumes of each fraction were used for Western blot analysis.
Fig 2: Exosomal EphB4 partially mediates the effect of SRGN on invasion of ESCC cells. (A) Validation of EPHB4-knockdown efficiency in ESCC cells with SRGN overexpression. (B) Effect of EPHB4-knockdown on pro-invasive ability of exosomes derived from SRGN-overexpressing ESCC cells. Scale bar, 200 µm. Data are presented as mean ± SD. n = 4. *, P < 0.05; ***, P < 0.001.
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