Fig 1: Time-dependent changes in CCR1, CCR3, CCL2, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, and CCL9 protein levels (A–J) in male mice on the 2nd, 12th and 28th days after chronic constriction injury of the sciatic nerve in the spinal cord. The Western blot/ELISA data are presented as the fold change of control ± SEMs. Intergroup differences were analyzed using one-way ANOVA with Bonferroni’s post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate a significant difference compared with the control group (naive). @@ p < 0.01 and @@@ p < 0.001 indicate differences between the 2nd vs. 12th, 2nd vs. 28th, and 12th vs. 28th days. The quantity of animals used in the experiment was as follows: Western blot: N (n = 5–6), 2d (n = 6), 12d (n = 6), 28d (n = 5–6); ELISA: N (n = 6), 2d (n = 5–6), 12d (n = 5–6), 28d (n = 4–5). Abbreviations: N—naive.
Fig 2: Time-dependent changes in CCL2, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, CCL9, CCL11, CCL24, CCL26 and CCL28 mRNA levels (A–L) in male mice on the 2nd, 7th, 14th and 28th days after chronic constriction injury of the sciatic nerve in the spinal cord. The RT–qPCR data are presented as the fold change of control ± SEMs. Intergroup differences were analyzed using one-way ANOVA with Bonferroni’s post hoc test for multiple comparisons. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate a significant difference compared with the control group (naive). @ p < 0.05, @@ p < 0.01, and @@@ p < 0.001 indicate differences between the 2nd vs. 7th, 2nd vs. 14th, 2nd vs. 28th, 7th vs. 14th, 7th vs. 28th, and 14th vs. 28th days. The quantity of animals used in the experiment was as follows: RT-qPCR: N (n = 5–7), 2d (n = 8), 7d (n = 8–10), 14d (n = 10), 28d (n = 8–9). Abbreviations: N—naive.
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