Fig 1: Influence of r-mTBI on cerebrovascular angiopoietin secretion. A Mfsd2a expression in HBMEC following treatment with Ang-1 (2.5 ng/ml) for 24 h at 37 °C. Ang-1 expression was quantified by ELISA and normalized to total protein using the BCA assay. Values represent mean ± SD (n = 4) and are expressed as ng Mfsd2a per µg of total protein. *P < 0.05 compared to vehicle as determined by unpaired t-Test. B Secretion of Ang-1 and Ang-2 from fresh cerebrovessels isolated from r-sham and r-mTBI hTau mice. Following 72 h of incubation at 37 °C, the cerebrovascular extracellular media was probed for Ang-1 and Ang-2 using an ELISA and normalized to total protein using the BCA assay. Values represent mean ± SD (n = 5) and are expressed as pg of Ang-1 or Ang-2 per mg total protein. *P < 0.05 compared to each respective r-sham as determined by a Mann–Whitney U test