Description
Description: This assay employs a two-site sandwich ELISA to quantitate HSPA7 in samples. An antibody specific for HSPA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress.Members of the Hsp70 family are strongly upregulated by heat stress and toxic chemicals, particularly heavy metals such as arsenic, cadmium, copper, mercury, etc. Hsp70 was originally discovered by FM Ritossa in the 1960s when a lab worker accidentally boosted the incubation temperature of Drosophila (fruit flies).
Heat shock 70kDa protein 7 (HSP70B) also known as HSPA7 is a human gene. The protein encoded by this gene is a member of the Hsp70 family of heat shock proteins