Fig 1: The scheme of the experiment. EMF, electromagnetic field; FSH, follicle-stimulating hormone; LH, luteinizing hormone; SHANK3, SH3 and multiple ankyrin repeat domain; IGF1, insulin-like growth factor-1; VEGF, vascular endothelial growth factor; H&E, hematoxylin-eosin; PAS, periodic acid-Schiff; SEM, scanning electron microscopy; MDA, malondialdehyde; GSH, glutathione.
Fig 2: (a); Immunostaining of rat testes with SHANK3, VEGF, IGF1, and caspase-3 antibodies. (A, D, G, J—Sham group; B, E, H, K—EMF-28 group; C, F, I, L—EMF-42 group). Black arrows, immunopositive cells. EMF, 50 Hz electromagnetic field exposure (4 h/day, 5 days/week for 7 (EMF-28) and 9 (EMF-42) weeks); Sham group was exposed to the same conditions as the EMF group, except for the EMF exposure. Scale bar, 50 µm. SHANK3, VEGF, IGF1, and caspase-3 immunohistochemistry scores of Sham (n = 7), EMF-28 (n = 7), and EMF-42 (n = 7) group rat pups’ testes (one-way ANOVA with subsequent LSD multiple comparisons test). * p < 0.0001, significantly different from EMF-28 group (b), * p < 0.0001, significantly different from EMF-42 group (c–e).
Supplier Page from MyBioSource.com for Rat SH3 and Multiple Ankyrin Repeat Domains Protein 3 (SHANK3) ELISA Kit