ProNex Size-Selective Purification System NG2001 from Promega

Product Specs
ItemProNex Size-Selective Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberNG2001
Quantity10ml
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Digital telomere measurement by long-read sequencing distinguishes healthy aging from diseasebioRxivDecember 1, 2023Santiago E. Sanchez, Jessica Gu, Anudeep Golla, Annika Martin, William Shomali, Dirk Hockemeyer, Sharon A. Savage, Steven E. ArtandiEnd-prepped DNA was extracted from the reaction using Promega ProNex size selection beads (Catalog #NG2001) at a bead-to-reaction solution ratio of 1.6 and incubated on a Hula mixer at room temperature for five

(2) LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversificationScience AdvancesNovember 3, 2023Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski, et al.New England Biolabs), the Iso-Seq Express Oligo Kit (catalog no. PN-101737500, PacBio), ProNex beads [catalog nos. NG2001 (10 ml), NG2002 (125 ml), and NG2003 (500 ml), Promega] and the SMRTbell Express Template Prep Kit 2.0

(3) Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflowsPlant MethodsOctober 21, 2023Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem, Robert J. Henryquantity of 160 ng for library preparation. The amplified cDNA was filtered with ProNex beads (Promega, NG2001) following the Iso-Seq protocol into two libraries using two different workflows: standard and long

(4) Einkorn genomics sheds light on history of the oldest domesticated wheatNatureAugust 2, 2023Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero, et al.Amplification Module (New England Biolabs, E6421S). The ProNex Size-Selective Purification System (Promega, NG2001) was used for size selection. One SMRT Cell 8M (Pacific Biosciences, 101-389-001) was sequenced on the

(5) Efficient and sensitive profiling of RNA–protein interactions using TLC-CLIPNucleic Acids ResearchJuly 21, 2023Christina Ernst, Julien Duc, Didier Tronotube. Size-selection of cDNA was performed using ProNEX® Size-Selective Purification System (Promega, #NG2002) with a ratio of 2.8X to enrich for cDNA inserts of at least 20 nucleotides in length (>80 bp). Library

(6) Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencingbioRxivJuly 12, 2023Anupama Jha, Stephanie C. Bohaczuk, Yizi Mao, Jane Ranchalis, Benjamin J. Mallory, Alan T. Min, Morgan O. Hamm, Elliott Swanson, Connor Finkbeiner, Tony Li, et al.cat#M0222S), EcoRI methyltransferase (NEB, M0211S), ProNex® Size-Selective Chemistry (Promega, cat#NG2001), PacBio Elution buffer (PacBio #101-633-500), ZORBAX Eclipse Plus C18 (Agilent, cat#959757-902). All

(7) Application of a Spacer-nick Gene-targeting Approach to Repair Disease-causing Mutations with Increased SafetyBIO-PROTOCOLApril 20, 2023Ngoc Tung Tran, Mikhail Lebedin, Eric Danner, Ralf Kühn, Klaus Rajewsky, Van Trung Chu(Promega, catalog number: A1120)ProNex size-selective purification beads (Promega, catalog number: NG2001)High sensitivity DNA BioAnalyzer kit (Agilent, catalog number: 5067-4626)TOP10 bacteria (Invitrogen,

(8) A chromosome-level reference genome and pangenome for barn swallow population genomicsCell ReportsJanuary 31, 2023Simona Secomandi, Guido R. Gallo, Marcella Sozzoni, Alessio Iannucci, Elena Galati, Linelle Abueg, Jennifer Balacco, Manuela Caprioli, William Chow, Claudio Ciofi, et al.for both brain and ovary. Amplified cDNA was cleaned with ProNex Beads (Promega - Catalog numbers: NG2001). For each sample, a PacBio library was prepared using the Pacific Biosciences SMRTbell Express Template

(9) Cooperation of chromatin remodeling SWI/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapesNature Structural & Molecular BiologyJanuary 1, 2023Bennett K. Wolf, Yanding Zhao, Andrew McCray, William H. Hawk, Luke T. Deary, Nicholas W. Sugiarto, Ian S. LaCroix, Scott A. Gerber, Chao Cheng, Xiaofeng WangPhenol:Chloroform:Isoamyl Alcohol (Cat.# 15593031, ThermoFisher) and purified using ProNex Purification System (Cat.#NG2003, Promega). CUT&RUN library was prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (Cat.#

(10) Studying miRNA–mRNA Interactions: An Optimized CLIP-Protocol for Endogenous Ago2-ProteinMethods and ProtocolsNovember 30, 2022Sophie Stebel, Janina Breuer, Oliver RossbachCTGAACCGCTCTTCCGATCT [1]);ProNex Size-Selection Purification System (Promega Corporation, Madison, WI, USA; NG2001);Rotiphorese Gel 40 (19:1) (Carl Roth GmbH + Co. KG, Karlsruhe, Germany; Cat. no.: 3030.1);DNA loading

(11) MicroRNAs are deeply linked to the emergence of the complex octopus brainScience AdvancesNovember 25, 2022Grygoriy Zolotarov, Bastian Fromm, Ivano Legnini, Salah Ayoub, Gianluca Polese, Valeria Maselli, Peter J. Chabot, Jakob Vinther, Ruth Styfhals, Eve Seuntjens, et al.of PCR amplification, and performing size selection after amplification with ProNex beads (Promega #NG2001), to enrich transcripts larger than 3 kb. Iso-Seq libraries were then also multiplexed and sequenced

(12) Trans-regulatory changes underpin the evolution of the Drosophila immune responsePLoS GenetNovember 7, 2022Shuai Dominique Ding, Alexandre B. LeitÃ£o, Jonathan P. Day, Ramesh Arunkumar, Morgan Phillips, Shuyu Olivia Zhou, Francis M. Jigginsvolumes of PCR product. 30 μL of each pool was purified by using 3x volumes of ProNex beads (Promega NG2001) and processing according to the manufacturer’s recommendations. The purified pools were eluted from

(13) Spatially mapping T cell receptors and transcriptomes reveals distinct immune niches and interactions underlying the adaptive immune responseImmunityOctober 11, 2022Sophia Liu, J. Bryan Iorgulescu, Shuqiang Li, Mehdi Borji, Irving A. Barrera-Lopez, Vignesh Shanmugam, Haoxiang Lyu, Julia W. Morriss, Zoe N. Garcia, Evan Murray, et al.min, and 72°C for 2 min, and then hold at 4°C. Then 19.2 μL of ProNex beads (0.8× clean-up; Promega NG2003) were added for clean-up following the manufacturer’s instructions and eluted into 20 μL of ProNex Elution Buffer

(14) Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancerNature structural & molecular biologySeptember 1, 2022Sugimoto Y, Ratcliffe PJ.4: multiplexing and optimized PCR cycle amplification After adding 37.5 µL ProNex beads (Promega, NG2002) to each sample, up to 16 samples were multiplexed. The cDNA library was purified according to the manufacturer’s

(15) A transcriptional roadblock protects yeast centromeresNucleic Acids ResearchAugust 12, 2022Sabrine Hedouin, Glennis A Logsdon, Jason G Underwood, Sue Bigginswas incubated at 20°C for 1 h. The SMRTbell libraries were cleaned up with ProNex beads (Promega, #NG2001), pooled, and sequenced on a SMRT Cell 8M on the Pacific Biosciences Sequel II in CCS mode with the

(16) NMR-derived secondary structure of the full-length Ox40 mRNA 3′UTR and its multivalent binding to the immunoregulatory RBP RoquinNucleic Acids ResearchApril 22, 2022Jan-Niklas Tants, Lea Marie Becker, François McNicoll, Michaela Müller-McNicoll, Andreas Schlundtbarcodes. PCR libraries were purified using the ProNex® Size-Selective Purification System (Promega NG2001) removing primers and cDNAs, and sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired end

(17) Long-read genome sequencing of bread wheat facilitates disease resistance gene cloningNature GeneticsMarch 14, 2022Naveenkumar Athiyannan, Michael Abrouk, Willem H. P. Boshoff, Stéphane Cauet, Nathalie Rodde, David Kudrna, Nahed Mohammed, Jan Bettgenhaeuser, Kirsty S. Botha, Shannon S. Derman, et al.Amplification Module (New England Biolabs, E6421S) and the ProNex Size-Selective Purification System (Promega, NG2001) for size selection. In brief, 300 ng of total RNA from each of the six developmental stages was used

(18) Butterfly eyespots evolved via cooption of an ancestral gene-regulatory network that also patterns antennae, legs, and wingsProceedings of the National Academy of SciencesFebruary 22, 2022Suriya Narayanan Murugesan et al.fragments were size selected between 50 and 1,500 bp, using the ProNex Size-Selective Purification System (NG2002) from Promega. The samples were sequenced, using NovosEq. 6000 with an average read depth of 30 million

(19) S146L in MYC is a context-dependent activating substitution in cancer developmentPloS oneJanuary 1, 2022Hinds JW, Feris EJ, Wilkins OM, Deary LT, Wang X, Cole MD.performed using 15 cycles of amplification PCR. Size selection was performed using ProNex beads (Promega #NG2001) to collect library fragments ~150bp to ~750bp. Prepared libraries were submitted to the Dartmouth Genomics

(20) MegaGate: A toxin-less gateway molecular cloning toolSTAR ProtocolsDecember 17, 2021Christian Kramme, Alexandru M. Plesa, Helen H. Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E. Kohman, Pranam Chatterjee, George M. ChurchR0739 SapI,New England Biolabs,Cat: R0569 ProNex Size-Selective Purification System,Promega,Cat: NG2001 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Qubit dsDNA HS Assay Kit

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ProNex Size-Selective Purification System

ProNex Size-Selective Purification System from Promega

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ProNex Size-Selective Purification System from Promega

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