Description
This Eagle Biosciences Fecal C. Difficile Toxin A ELISA Assay Kit is a “sandwich” ELISA is designed, developed and produced for the qualitative measurement of Toxin A in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells. Controls and extracted fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal anti-Toxin A on its wall. During the assay, the Toxin A Antibody will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of Toxin A is added for further immunoreactions. After an incubation period, the immunocomplex of “Anti-Toxin A Capture Antibody – C. Diff. Toxin A – HRP-conjugated Anti-Toxin A Tracer Antibody” is formed if Toxin A is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Toxin A proteins captured on the wall of each microtiter well is directly proportional to the amount of Toxin A level in each test specimen