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Assay Kit
HIV-1 p24 ELISA Kit, 96 wells
HIV-1 p24 ELISA Kit, 96 wells from XpressBio
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Citations:
(18)
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HIV-1 p24 ELISA Kit, 96 wells
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Description
The assay is used to monitor HIV-1 in cell culture or to determine the viral titer of lentiviral samples
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
HIV-1 p24 ELISA Kit, 96 wells
Company
XpressBio
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
XB-1000
Quantity
96 tests
Applications
ELISA
Detection Target
HIV-1 p24
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XpressBio
4650 Wedgewood Blvd
Suite 103
Frederick, MD
21703
United States
Phone
: + 301-228-2444
Fax: + 301-560-6570
US Toll Free: 888-562-8914
Email: info@xpressbio.com
Company Profile
Website:
www.xpressbio.com/
Citations (18)
(1)
MicroRNA-25/93 induction by Vpu as a mechanism for counteracting MARCH1-restriction on HIV-1 infectivity in macrophages
mBio
October 31, 2023
Robert Lodge, Zaikun Xu, Mckenna Eklund, Christina Stürzel, Frank Kirchhoff, Michel J. Tremblay, Tom C. Hobman, Éric A. Cohen
Fluorochromed Alexa secondary antibodies were from Invitrogen. HIV-1 p24 was measured by
ELISA
(
XpressBio
#
XB-1000
). Additionally, the following reagents were used: HIV-1 integrase inhibitor
Raltegravir
(
Santa Cruz Biotechnology
(2)
The Effect of Select SARS-CoV-2 N-Linked Glycan and Variant of Concern Spike Protein Mutations on C-Type Lectin-Receptor-Mediated Infection
Viruses
September 9, 2023
Arjan Bains, Wenyan Guan, Patricia J. LiWang
using an XpressBio p24 ELISA kit plate according to the manufacturer’s instructions (
XpressBio
Cat #
XB-1000
, Frederick, MD, USA). The levels of p24 in each well were measured on a
ClarioStar Plus
microplate reader
(3)
Chemical inhibition of DPP9 sensitizes the CARD8 inflammasome in HIV-1-infected cells
Nature Chemical Biology
April 1, 2023
Kolin M. Clark, Josh G. Kim, Qiankun Wang, Hongbo Gao, Rachel M. Presti, Liang Shan
measurement, and dose response HIV-1 p24 ELISA was used to verify viral stock concentration (
XpressBio
#
XB-1000
).
HIV-1
reporter
virus infection
was performed at a multiplicity of
infection
(MOI) of 10.
Infection
(4)
Antibody gene transfer treatment drastically improves epidermal pathology in a keratitis ichthyosis deafness syndrome model using male mice
eBioMedicine
March 1, 2023
Chiara Peres, Caterina Sellitto, Chiara Nardin, Sabrina Putti, Tiziana Orsini, Chiara Di Pietro, Daniela Marazziti, Adriana Vitiello, Arianna Calistri, Mara Rigamonti,
et al.
abEC1.1 were quantified by measuring the amount of the
HIV-1
capsid p24 protein by ELISA assay (Cat. No.
XB-1000
,
XpressBio
, Frederick, MD, USA). Titer of RLPs was then expressed in transducing units (TU)/ml by converting
(5)
Identification of Novel Nucleocapsid Chimeric Proteins Inhibiting HIV-1 Replication
International Journal of Molecular Sciences
October 15, 2022
Hae-In Kim, Ga-Na Kim, Kyung-Lee Yu, Seong-Hyun Park, Ji Chang You
ELISA
ELISA
for HIV-1 p24 was performed according to the manufacturer’s instructions (
XpressBio
, Cat. #
XB-1000
). Briefly, we serially diluted 10 μL of each harvested viral supernatant 10-fold in cultured medium.
(6)
SARS-CoV-2 pseudotyped virus persists on the surface of multiple produce but can be inactivated with gaseous ozone
Heliyon
August 15, 2022
Mehrad Mortazavi, Arjan Bains, Leili Afsah-Hejri, Reza Ehsani, Patricia J. LiWang
the top. Then 10 μL of 10x XpressBio Lysis Buffer (provided in HIV-1 p24 ELISA Kit,
XpressBio
Cat #
XB-1000
, Frederick, MD, USA) was added over the top and allowed to sit for 5 min to ensure complete p24 release.
(7)
Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
PLOS ONE
July 8, 2022
Natalia Alvarez, Sandra M. Gonzalez, Juan C. Hernandez, Maria T. Rugeles, Wbeimar Aguilar-Jimenez
medium supplemented with 10% FBS and 1% antibiotic. Levels of p24 were measured using the ELISA Kit
XB-1000
(
XpressBio
, Frederick, MD), 3- and 7-day post-
infection
. 2.6.
Flow cytometry
For each
assay
, cell
(8)
Site-Specific and Stable Conjugation of the SARS-CoV-2 Receptor-Binding Domain to Liposomes in the Absence of Any Other Adjuvants Elicits Potent Neutralizing Antibodies in BALB/c Mice
Bioconjugate chemistry
December 15, 2021
Wholey WY, Yoda ST, Cheng W.
°C freezer. The concentration of virion particles was quantitated using an HIV-1 p24 ELISA kit (CAT#
XB-1000
,
XpressBio
) as we described previously.40 Virion
Neutralization Assay
. Virion
neutralization assay
(9)
Lentiviral Vectors Expressing Chimeric NEDD4 Ubiquitin Ligases: An Innovative Approach for Interfering with Alpha-Synuclein Accumulation
Cells
November 21, 2021
Stefania Vogiatzis, Michele Celestino, Marta Trevisan, Gloria Magro, Claudia Del Vecchio, Deran Erdengiz, Giorgio Palù, Cristina Parolin, Kathleen Maguire-Zeiss, Arianna Calistri
dilutions [29]. RLPs expressing ubiquibodies were titrated by adopting the HIV-1 p24 ELISA kit (
XpressBio
,
XB-1000
), following manufacturer’s instructions. Briefly, 4 μL of concentrated supernatant were serially diluted
(10)
SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2
Immunity
June 8, 2021
Lu Q, Liu J, Zhao S, Gomez Castro MF, Laurent-Rolle M, Dong J, Ran X, Damani-Yokota P, Tang H, Karakousi T,
et al
.
Immunology
Multiplex Assay
,
Millipore
,Cat#:
HCYTMAG-60K-PX30
HIV-1 p24 ELISA
Assay
,
XpressBio
,Cat#:
XB-1000
Q Buffer,Probelife,Cat#: 120010 HFC (Anti-HIgG Fc) Probe,Probelife,Cat#: 160003 ,, Deposited data,Deposited
(11)
Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments
Cell reports
August 18, 2020
Rujas E, Insausti S, Leaman DP, Carravilla P, González-Resines S, Monceaux V, Sánchez-Eugenia R, García-Porras M, Iloro I, Zhang L,
et al
.
EasySep Human CD4 positive selection kit II
,
Stem Cell
Technology,Cat#
17852
HIV-1 p24
enzyme-linked immunosorbent assay
,
XpressBio
,Cat#
XB-1000
Experimental Models
: Cell Lines,
Experimental Models
: Cell Lines,
Experimental Models
: Cell Lines
Human
:
(12)
HIV protease cleaves the antiviral m6A reader protein YTHDF3 in the viral particle
PLoS pathogens
February 1, 2020
Jurczyszak D, Zhang W, Terry SN, Kehrer T, Bermúdez González MC, McGregor E, Mulder LCF, Eckwahl MJ, Pan T, Simon V.
transfection
, viral supernatants were harvested and used to quantify Gag p24 using HIV-1 p24 ELISA (
XpressBio
#
XB-1000
) following manufacturer's instructions and determine viral infectivity. Replication-competent
HIV
NL4-3
(13)
Chromatin maturation of the HIV-1 provirus in primary resting CD4+ T cells
PLoS pathogens
January 1, 2020
Lindqvist B, Svensson Akusjärvi S, Sönnerborg A, Dimitriou M, Svensson JP.
GFP signals with
flow cytometry
. We determined
virus
titers by the HIV-1 p24 ELISA
Assay
(
XpressBio
, Cat# Cat#
XB-1000
).
Virus
-containing supernatant was concentrated with LentiXconcentrator (Clontech, Cat# 631231). Lentiviral
(14)
Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity
Cell reports
November 5, 2019
Jin SW, Alsahafi N, Kuang XT, Swann SA, Toyoda M, Göttlinger H, Walker BD, Ueno T, Finzi A, Brumme ZL,
et al
.
165–2670
96-Well
Electroporation
Plates,
Bio-Rad
,Cat #
1652681
HIV-1 p24 ELISA Kit,
XpressBio
,Cat #
XB-1000
Steady-Glo® Luciferase Assay System
,
Promega
,Cat #
E2550
Deposited Data,,
HIV-1
subtype B Nef sequences,"Mwimanzi
(15)
Expression of HERV-K108 envelope interferes with HIV-1 production
Virology
September 1, 2017
Sandra N. Terry, Lara Manganaro, Alvaro Cuesta-Dominguez, Daria Brinzevich, Viviana Simon, Lubbertus C. F. Mulder
after
transfection
supernatants were harvested and p24 was measured using HIV-1 p24 ELISA (
XpressBio
#
XB-1000
) following manufacturer’s instructions. Measurement of viral infectivity TZM-bl reporter cell-line
(16)
Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate
Vaccine
June 1, 2016
Alina Venereo-Sanchez, Renald Gilbert, Melanie Simoneau, Antoine Caron, Parminder Chahal, Wangxue Chen, Sven Ansorge, Xuguang Li, Olivier Henry, Amine Kamen
negative control. 169 The concentration of p24 was determined using the HIV-1 p24 ELISA
assay
cat#
XB-1000
(
XpressBio
, 170 USA). 171 2.14.
Transmission electron microscopy
(TEM) 172 Transmission electron
(17)
High-content analysis of α-synuclein aggregation and cell death in a cellular model of Parkinson's disease
Journal of Neuroscience Methods
March 1, 2016
Francesca Macchi, Angélique Deleersnijder, Chris Van den Haute, Sebastian Munck, Hans Pottel, Annelies Michiels, Zeger Debyser, Melanie Gerard, Veerle Baekelandt
p24 antigen content by an
enzyme linked immunosorbent assay
(HIV-1 p24 ELISA kit,
XpressBio
Europe Cat No.
XB-1000
). Prior to transduction, 1.5 × 105 SH-SY5Y cells were plated in a 24-well plate and grown in DMEM-complete.
(18)
Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity
Stem Cell Research & Therapy
December 1, 2015
Ran Kang, Yan Zhou, Shuang Tan, Guangqian Zhou, Lars Aagaard, Lin Xie, Cody Bünger, Lars Bolund, Yonglun Luo
phosphate-buffered saline (PBS) and stored at −80 °C.
Virus titer
was measured with the P24 Elisa kit (
XB-1000
,
XpressBio
).
Lentivirus
-mediated
reprogramming
NHDFs (1.5 × 105 cells/per well) were seeded in a six-well
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HIV-1 p24 ELISA Kit, 96 wells from XpressBio
Supplier Page
View Company Product Page
Pricing Info
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