Fig 1: Levels of IL-6, CSF3, MMP8, and S100A8 and ROC curves of S100A8 and MMP8 in the peripheral blood of SAE patients. (A) When compared to the non-SAE (n=10) group, the SAE patients (n=21) had higher serum levels of MMP8 and S100A8 (both p<0.0). (B) High diagnostic accuracy was shown for the identification of SAE by both S100A8 (AUC = 0.962, 95% CI = 0.9001-1.000) and MMP8 (AUC = 0.7905, 95% CI = 0.6245-0.9564), with S100A8 having the highest accuracy. (C, D) Correlation analysis of the four extracellular molecules with clinical indicators and prognostic markers. S100A8, MMP8, CSF3, and IL-6 protein levels in the peripheral blood of sepsis patients were significantly linked with GCS scores (all p<0.05). S100A8 levels and 28-day mortality were strongly associated (r=0.634, p<0.01), and IL-6 levels and duration of mechanical breathing were positively correlated (r=0.360, p=0.046). *p <0.05, **p <0.01, ****p <0.0001; ns, no significance.
Fig 2: Levels of MMP8, and S100A8 in the peripheral blood and CSF of sepsis rats. (A)Timeline of experiment procedures. (B) The recognition index of novel items in the CLP model rats (n =11) was lower than that of the Sham group rats (p < 0.05 vs Sham, n = 8). (C) In the Barnes maze experiment, the CLP model rats (n =11) made significantly more mistakes before finding the target hole on postoperative day 9 and 10 compared to the Sham group rats (p < 0.01, p < 0.05 vs Sham, n = 8). (D) The CLP rats (n=14) exhibited significantly elevated serum levels of MMP8 (p<0.05) and CSF levels (p=0.0506) in comparison to the SHAM group (n=12). (E) When compared to the SHAM (n=14) group, the CLP rats (n=12) had higher serum levels of S100A8(p<0.01) and CSF levels (all p<0.0001). CLP, cecal ligation and puncture; NS., no significance; NOR, novel object recognition. *p <0.05; ****p <0.0001.
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