Description
The Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set is for the quantitative determination of Human TNF-alpha / TNFA / TNFSF2. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human TNF-alpha / TNFA / TNFSF2 coated on a 96-well plate. Standards and samples are added to the wells, and any Human TNF-alpha / TNFA / TNFSF2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human TNF-alpha / TNFA / TNFSF2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm