Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set from Sino Biological, Inc.

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Sino Biological, Inc. for
Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set

Description

The Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set ELISA Pair Set is for the quantitative determination of Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm