Fig 1: Generation of DTVRF-hiPSC-ARGs via seeding of PCL scaffolds with patient iPSC-derived retinal progenitor cells. (A–E) Immunocytochemical staining of hiPSC-derived retinal organoids at differentiation days 80 (A—time of dissociation and scaffold seeding) and 150 (B–E). Antibodies targeting the retinal progenitor cell markers SOX2 and CHX10, the rod photoreceptor cell markers NR2E3, NRL, and rhodopsin, and the cone photoreceptor cell markers ARR3 and blue cone opsin. DAPI was used as a nuclear counterstain. (F) Phase micrograph of DTVRF-hiPSC-ARG 2 weeks following seeding. Note the uniform seeding of pores across the entire scaffold. (G–J) Immunocytochemical staining of DTVRF-hiPSC-ARG 2 weeks following seeding. Antibodies targeting OTX2 (G and H—green) and Recoverin (I and J—green) were used to confirm the presence of photoreceptor precursor cells. Red = Sytox™ nuclear stain. Scale bar = 200 µm (F) and 50 µm (I). The scaffold material autofluoresces blue-magenta under these conditions. PCL: polycaprolactone; iPSC: induced pluripotent stem cells; hiPSC: human induced pluripotent stem cell.