ddSEQ™ Single-Cell Isolator from Bio-Rad

Product Specs
ItemddSEQ™ Single-Cell Isolator
CompanyBio-Rad
Price Get Pricing
Catalog Number12004336
TypeSingle-Cell Isolator
ThroughputProcess hundreds to tens of thousands of cells per day
Data AnalysisAnalysis is conducted via BaseSpace
Brand/TrademarkLSG DBG

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) The single-cell chromatin accessibility landscape in mouse perinatal testis developmenteLifeApril 25, 2023Hoi Ching Suen, Shitao Rao, Alfred Chun Shui Luk, Ruoyu Zhang, Lele Yang, Huayu Qi, Hon Cheong So, Robin M Hobbs, Tin-lap Lee, Jinyue Liaoice before encapsulation. Library preparation and sequencing Tagmented nuclei were loaded onto a ddSEQ Single-Cell Isolator (Bio-Rad). scATAC-Seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620,

(2) Cellular senescence in malignant cells promotes tumor progression in mouse and patient GlioblastomaNature CommunicationsJanuary 27, 2023Rana Salam, Alexa Saliou, Franck Bielle, Mathilde Bertrand, Christophe Antoniewski, Catherine Carpentier, Agusti Alentorn, Laurent Capelle, Marc Sanson, Emmanuelle Huillard, et al.wells with GFP+ cells or malignant cells and 1 well with GFP− cells or non-malignant cells) on the ddSEQ Single-Cell Isolator (Biorad). A library was generated using SureCell™ Whole Transcriptome Analysis 3′ Library Prep Kit for

(3) scATAC-Seq reveals heterogeneity associated with spermatogonial differentiation in cultured male germline stem cellsScientific ReportsDecember 12, 2022Hoi Ching Suen, Alfred Chun Shui Luk, Jinyue Liaoencapsulation. scATAC-seq library preparation and sequencing Tagmented nuclei were loaded onto a ddSEQ Single-Cell Isolator (Bio-Rad). scATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620,

(4) Recent advances in targeted drug delivery systems for resistant colorectal cancerCancer Cell InternationalDecember 1, 2022Masoumeh Sharifi-Azad, Marziyeh Fathi, William C. Cho, Abolfazl Barzegari, Hamed Dadashi, Mehdi Dadashpour, Rana Jahanban-Esfahlanselection of sequencing platform such as 0X Genomics Chromium, Nadia (Dolomite Bio), Illumina Bio-Rad ddSEQ Single-Cell Isolator, BD Rhapsody Single-Cell Analysis System (BD), ICELL8 Single-cell System (Takara), and Fluidigm C1,

(5) Functional inference of gene regulation using single-cell multi-omicsCell GenomicsSeptember 1, 2022Vinay K. Kartha et al.SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Tagmented cells were loaded onto a ddSEQ Single-Cell Isolator (12004336, Bio-Rad). Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit

(6) A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal CancerPathology and Oncology ResearchJuly 14, 2022William J. Kothalawala, Barbara K. Barták, Zsófia B. Nagy, Sára Zsigrai, Krisztina A. Szigeti, Gábor Valcz, István Takács, Alexandra Kalmár, Béla Molnárbarcoded beads thus enabling parallel sorting of cells and library preparation for NGS. The Bio-Rad ddSEQ Single-Cell Isolator (Bio-Rad Laboratories, Hercules, California, United States) offers a similar technique. Both platforms

(7) Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypesDevelopmentMarch 1, 2022Behzad Yaghmaeian Salmani, Brad Balderson, Susanne Bauer, Helen Ekman, Annika Starkenberg, Thomas Perlmann, Michael Piper, Mikael Bodén, Stefan Thorwere encapsulated into droplets using Bio-Rad ddSEQ Single-Cell Isolator for cell lysis and barcoding (12004336). Subsequently, RNA-seq libraries were generated using SureCell WTA 3′ Library Prep Kit for the ddSEQ

(8) Expression of Lineage Transcription Factors Identifies Differences in Transition States of Induced Human Oligodendrocyte DifferentiationCellsJanuary 11, 2022Florian J. Raabe et al.single-cell libraries were prepared with the SureCell WTA 3′ Kit (Illumina, San Diego, CA, USA) on a ddSEQ Single-Cell Isolator (Bio-Rad, Hercules, USA) in accordance with the manufacturer’s protocol. Library quality and yield were

(9) Circulating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapyNature CommunicationsDecember 1, 2021Sara De Biasi et al.mW). Cell sorting was performed with 96-99% purity. Sorted CD3+CD8+ T cells were immediately loaded on ddSEQ single-cell isolator (Bio-Rad Laboratories) to isolate single cells and barcode single cells. sc-RNA-seq libraries were prepared

(10) Deep learning-based enhancement of epigenomics data with AtacWorksNature CommunicationsDecember 1, 2021Avantika Lal, Zachary D. Chiang, Nikolai Yakovenko, Fabiana M. Duarte, Johnny Israeli, Jason D. Buenrostromin at 37 °C. Tagmented cells were kept on ice before being loaded onto a ddSEQ Single-Cell Isolator (12004336, Bio-Rad). scATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620,

(11) What are the applications of single-cell RNA sequencing in cancer research: a systematic reviewJournal of Experimental & Clinical Cancer ResearchDecember 1, 2021Lvyuan Li et al.single-cell sequencing platforms include the 10X Genomics Chromium, Nadia (Dolomite Bio), Illumina Bio-Rad ddSEQ Single-Cell Isolator, BD Rhapsody Single-Cell Analysis System (BD), ICELL8 Single-cell System (Takara), Fluidigm C1, and

(12) Chromatin accessibility profiling methodsNature Reviews Methods PrimersDecember 1, 2021Liesbeth Minnoye et al.the acquisition of proprietary robotic sample processing devices (Chromium Controller, 10x Genomics; ddSEQ single-cell isolator, BioRad) that are non-standard in most laboratories. Droplet microfluidic-based scATAC-seq methods generally

(13) HER2 Isoforms Uniquely Program Intratumor Heterogeneity and Predetermine Breast Cancer Trajectories During the Occult Tumorigenic PhaseMolecular Cancer ResearchOctober 1, 2021Joshua D. Ginzel et al.filter. Single cells were resuspended in 0.1% BSA at 3000 cells/μL. ddSEQ Single-Cell Isolator (Biorad 12004336) was used to capture single cells according to manufacturer’s protocol. Library preparation was performed

(14) Hyaluronic acid is a negative regulator of mucosal fibroblast-mediated enhancement of HIV infectionMucosal immunologySeptember 1, 2021Egedal JH, Xie G, Packard TA, Laustsen A, Neidleman J, Georgiou K, Pillai SK, Greene WC, Jakobsen MR, Roan NR.subjected to scRNAseq through Biorad’s ddSeq system. The Illumina/Biorad SureCell 3′ WTA kit and BioRad’s ddSeq single-cell isolator were used for the preparation of single-cell RNA libraries according to the manufacturer’s protocol.

(15) Pluripotent stem cell-derived epithelium misidentified as brain microvascular endothelium requires ETS factors to acquire vascular fateProceedings of the National Academy of Sciences of the United States of AmericaFebruary 23, 2021Lu TM, Houghton S, Magdeldin T, Durán JGB, Minotti AP, Snead A, Sproul A, Nguyen DT, Xiang J, Fine HA, et al.distance. Single-Cell RNA-Seq Digital Droplet Sequencing (ddSeq). A single-cell suspension was loaded into the Bio-Rad ddSeq Single-Cell Isolator on which cells were isolated, lysed, and barcoded in droplets. Droplets were then disrupted, and cDNA

(16) Unveiling functional heterogeneity in breast cancer multicellular tumor spheroids through single-cell RNA-seqScientific ReportsDecember 1, 2020Erick Andrés Muciño-Olmos, Aarón Vázquez-Jiménez, Ugo Avila-Ponce de León, Meztli Matadamas-Guzman, Vilma Maldonado, Tayde López-Santaella, Abrahan Hernández-Hernández, Osbaldo Resendis-AntonioBSA in PBS, and at least 6 µL of these final dilution suspension were needed as Input for the Bio-Rad ddSEQ Single-Cell Isolator (Illumina Bio-Rad SureCell WTA 3′ Library Prep). As viability and cell numbers are critical, three technical

(17) Single-cell RNA sequencing reveals a heterogeneous response to Glucocorticoids in breast cancer cellsCommunications BiologyDecember 1, 2020Jackson A. Hoffman, Brian N. Papas, Kevin W. Trotter, Trevor K. Archersuspensions for scRNAseq, cells were dissociated using 0.25% Trypsin-EDTA (Gibco 25200). The BioRad ddSeq Single-Cell Isolator was used to create single-cell emulsions and the Illumina SureCell WTA 3′ Library Prep kit was used

(18) Single-cell RNA-seq identifies unique transcriptional landscapes of human nucleus pulposus and annulus fibrosus cellsScientific ReportsDecember 1, 2020Lorenzo M. Fernandes, Nazir M. Khan, Camila M. Trochez, Meixue Duan, Martha E. Diaz-Hernandez, Steven M. Presciutti, Greg Gibson, Hicham Drissimicrofluidic cartridges to co-encapsulate single cells and barcodes into sub-nanoliter droplets on a BioRad ddSEQ Single-Cell Isolator. Cell lysis, barcoding, and 3′ RNA Seq libraries generated with an Illumina SureCell WTA 3′ Library

(19) Toll-like receptor signaling in thymic epithelium controls monocyte-derived dendritic cell recruitment and Treg generationNature CommunicationsDecember 1, 2020Matouš Vobořil et al.Total cell concentration and viability was ascertained using a TC20 Automated Cell Counter (Bio-Rad). A ddSEQ Single-Cell Isolator (Bio-Rad) was used to co-encapsulate single cells with barcodes and enzyme solutions for cDNA synthesis.

(20) Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure DevelopmentCell ReportsDecember 1, 2020Sherina Malkani et al.tagmented cells were kept on ice. Tagmented cells were loaded onto the ddSEQ Single-Cell Isolator (12004336, Bio-Rad). The SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360,

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