High Pure Viral RNA Kit from Roche

Product Specs
ItemHigh Pure Viral RNA Kit
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number11858882001
Quantity1 kit (100 purifications)
SourceBlood, Plasma/Serum
Nucleic Acid TypeViral RNA

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) SEQUENCE ANALYSIS OF HOT SPOT REGIONS OF SPIKE AND RNA‑DEPENDENT‑RNA POLYMERASE (RDRP) GENES OF SARS-COV-2 IN KERMAN, IRANMediterranean Journal of Hematology and Infectious DiseasesJuly 1, 2023Nasir Arefinia, Ramin Yaghobi, Amin Ramezani, Jamal Sarvarifrom throat samples (one sample, one patient) using a highly pure Viral RNA Kit (Roche, Product no: 11858882001) based on manufacturer instructions. Primer design and reverse transcriptase polymerase chain reaction

(2) Comparison of Eleven RNA Extraction Methods for Poliovirus Direct Molecular Detection in Stool SamplesMicrobiology SpectrumApril 13, 2023Joyce Odeke Akello, Erika Bujaki, Alexander G. Shaw, Adnan Khurshid, Yasir Arshad, Catherine Troman, Manasi Majumdar, Áine O'Toole, Andrew Rambaut, Muhammad Masroor Alam, et al.for the Zymo quick RNA viral kit [Zymo, R1034], two protocols for the High Pure viral RNA kit [Roche, 11858882001], one each for the QIAamp viral RNA minikit [Qiagen, 52904], MagMAX viral RNA isolation kit [Thermo Fisher

(3) Genomic Analysis of a New Freshwater Cyanophage Lbo240-yong1 Suggests a New Taxonomic Family of BacteriophagesVirusesMarch 24, 2023Qin Zhou, Dengfeng Li, Wei Lin, Linting Pan, Minhua Qian, Fei Wang, Ruqian Cai, Chenxin Qu, Yigang TongGenomic DNA was extracted with the High Pure Viral RNA kit (Roche, Basel, Switzerland, product no. 11858882001), which allows for the extraction of DNA and RNA together. A 2 × 300 bp paired-end DNA library was constructed

(4) Circulating Plasma Exosomal Proteins of Either SHIV-Infected Rhesus Macaque or HIV-Infected Patient Indicates a Link to NeuropathogenesisVirusesMarch 21, 2023Chandra PK, Braun SE, Maity S, Castorena-Gonzalez JA, Kim H, Shaffer JG, Cikic S, Rutkai I, Fan J, Guidry JJ, et al.was extracted from 500 µL of plasma or exosomes suspension with the High Pure Viral RNA kit (cat. #11858882001; Roche, Indianapolis, IN, USA) according to the manufacture’s protocol. Reverse transcription was performed

(5) Rapid whole genome sequencing methods for RNA virusesFrontiers in MicrobiologyFebruary 23, 2023Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijoworking stock virus treated with the Micrococcal Nuclease using a High Pure Viral RNA Kit (catalog number 11858882001, Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions. The elution

(6) Impact of SIV infection on mycobacterial lipid-reactive T cell responses in Bacillus Calmette-Guérin (BCG) inoculated macaquesFrontiers in ImmunologyJanuary 16, 2023Edith M. Walker, Kristen M. Merino, Nadia Slisarenko, Brooke F. Grasperge, Smriti Mehra, Chad J. Roy, Deepak Kaushal, Namita Routseparation. SIV Plasma viral load quantification was performed using Roche High Pure Viral RNA Kit (Catalog #11858882001) as earlier described (34). The limit of detection of SIV RNA was 83 copies per milliliter of plasma. Blood

(7) Human parainfluenza 3 and respiratory syncytial viruses detected in pangolinsEmerging Microbes & InfectionsDecember 31, 2022Tengcheng Que et al.homogenate supernatant and eluted in 50 μL nuclease-free water using a High Pure viral RNA Kit (Cat. No. 11858882001 Roche, Switzerland) according to the manufacturer’s instructions. Reverse-transcription polymerase chain reaction

(8) Novel neutralizing chicken IgY antibodies targeting 17 potent conserved peptides identified by SARS-CoV-2 proteome microarray, and future prospectsFrontiers in ImmunologyDecember 22, 2022Jin Li, Te Liang, Ailian Hei, Xiangbin Wang, Huijun Li, Xiaobo Yu, Rui Zhao, Peng Gao, Cong Fang, Ji Zhou, et al.for the SARS-CoV-2 virus used to target both the ORF1ab and N-gene (ROCHE, High Pure Viral RNA Kit, #11858882001). The data were plotted after background subtraction and normalized to the controls. The virus-neutralizing

(9) Repeated Intravaginal Inoculation of Zika Virus Protects Cynomolgus Monkeys from Subcutaneous SuperchallengeInternational Journal of Molecular SciencesNovember 13, 2022Maya Shofa, Tomotaka Okamura, Emiko Urano, Yoshiharu Matsuura, Yasuhiro Yasutomi, Akatsuki SaitoPlasma Viral RNA Viral RNA was isolated from monkey plasma using a High Pure Viral RNA Kit (Roche, Cat# 11858882001), according to the manufacturer’s protocol. Viral RNA was quantified using a One Step TB Green PrimeScript

(10) Novel Aeromonas Phage Ahy-Yong1 and Its Protective Effects against Aeromonas hydrophila in Brocade Carp (Cyprinus aka Koi)VirusesNovember 11, 2022Lingting Pan, Dengfeng Li, Wei Lin, Wencai Liu, Chenxin Qu, Minhua Qian, Ruqian Cai, Qin Zhou, Fei Wang, Yigang TongThe phage genome was extracted from the filtrate using the High Pure Viral kit (Roche, Product No.: 11858882001, Basel, Switzerland). The genomic library was constructed with the NEBNextUltraTM II DNA Library Prep

(11) Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolatesVirologica SinicaSeptember 24, 2022Xiao-Li Feng et al.viral genomic RNA was extracted from viral culture supernatant using a high pure viral RNA kit (Roche, 11858882001, Germany) and purified according to the manufacturer's instructions. After that, the next-generation sequencing (NGS

(12) A Novel Freshwater Cyanophage Mae-Yong1326-1 Infecting Bloom-Forming Cyanobacterium Microcystis aeruginosaVirusesSeptember 16, 2022Fei Wang, Dengfeng Li, Ruqian Cai, Lingting Pan, Qin Zhou, Wencai Liu, Minhua Qian, Yigang TongRNA, then incubated at 80 °C for 15 min. High Pure Viral kitA high Pure Viral kit (Roche, Product No: 11858882001) was used to extract the cyanophage genome. NEB Next Ultra II DNA Library PrepKit (NEB, Product No:

(13) Establishment and application of a method of tagged-amplicon deep sequencing for low-abundance drug resistance in HIV-1Frontiers in MicrobiologyAugust 22, 2022Yang Li, Leilei Han, Yanglan Wang, Xiaolin Wang, Lei Jia, Jingyun Li, Jingwan Han, Jin Zhao, Hanping Li, Lin Liinvolved in this study was completed according to the following method. Roche High Pure Viral RNA Kit (REF:11858882001) was used to extract total viral RNA from plasma samples. The pol gene region (nucleotides positions

(14) Diagnostic accuracy of SARS-CoV-2 Panbio™ rapid antigen diagnostic tests in a 4,440-case clinical follow-upFrontiers in MedicineAugust 2, 2022Ágoston Hamar, Kristóf Filipánits, Alex Váradi, Rita Váradi-Rácz, Henrietta Orsolya Gellén, Krisztina Futács, Péter Urbán, Gabor L. Kovacs, Katalin Gombosprotocol. Additionally, during manual nucleic acid extraction, the HighPure RNA isolation kit (Cat No. 11858882001, Roche) was used in full compliance to the manufacturer's recommended protocol. Both automated and manual

(15) Trafficked Malayan pangolins contain viral pathogens of humansNature MicrobiologyAugust 2, 2022Wenqiang Shi et al.high-throughput sequencing, total RNA was extracted using a High Pure viral RNA kit (Roche Diagnostics, 11858882001), and viral RNA was then enriched by a Nucleic Acid Microbes purification kit (BGI PathoGenesis Pharmaceutical Technology

(16) First Characterization of a Hafnia Phage Reveals Extraordinarily Large Burst Size and Unusual Plaque PolymorphismFrontiers in MicrobiologyFebruary 8, 2022Lingting Pan et al.of filtrate was used for phage genome extraction with the High Pure Viral kit (Roche, Product No.: 11858882001). The kit allows the extraction of DNA and RNA together. To clarify the type and composition of the

(17) A Novel Freshwater Cyanophage, Mae-Yong924-1, Reveals a New FamilyVirusesJanuary 28, 2022Minhua Qian, Dengfeng Li, Wei Lin, Lingting Pan, Wencai Liu, Qin Zhou, Ruqian Cai, Fei Wang, Junquan Zhu, Yigang Tongcyanophage genome was extracted using a High Pure Viral RNA Kit (Roche, Basel, Switzerland, product no. 11858882001). This kit allows the extraction of DNA and RNA together. The NEBNext Ultra™ II DNA Library PrepKit

(18) Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virusEuropean Journal of Medical ResearchDecember 17, 2021Yushen Jiang et al.MagNA Pure 96 system (Roche, Germany) with the High Pure Viral RNA Kit (Roche, Germany. Product No. 11858882001). A LightMix® Modular EAV RNA Extraction Control (580) kit (Roche, Germany. Product No. 07654243001)

(19) A pathogen-like antigen-based vaccine confers immune protection against SARS-CoV-2 in non-human primatesCell reports. MedicineNovember 16, 2021Guo C, Peng Y, Lin L, Pan X, Fang M, Zhao Y, Bao K, Li R, Han J, Chen J, et al.A20173 THUNDERBIRD Probe One-Step qRT-PCR kit,TOYOBO,Cat# QRZ-101 High Pure Viral RNA Kit,Roche,Cat#11858882001 TRIzol Plus RNA Purification Kit,Thermo Fisher,Cat# A33254 MiniBEST Viral RNA/DNA Extraction Kit,TaKaRa,Cat#9766 PrimeScript

(20) High-Order Epistasis and Functional Coupling of Infection Steps Drive Virus Evolution toward Independence from a Host PathwayMicrobiology SpectrumOctober 31, 2021Minetaro Aritawas purified from the cells by using a High Pure viral RNA purification kit (Roche; product number 11858882001). Strand-specific real-time RT-PCR was performed as described previously (60, 67, 68), with modifications

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High Pure Viral RNA Kit from Roche

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High Pure Viral RNA Kit from Roche

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