T4 DNA Ligase from Promega

Product Specs
ItemT4 DNA Ligase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1804
Quantity500u
Concentration1–3u/μl

Specifications/Features

Unit Definition:
0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Programmable site-specific DNA double-strand breaks via PNA-assisted prokaryotic ArgonautesNucleic Acids ResearchSeptember 22, 2023Tin Marsic, Sivakrishna Rao Gundra, Qiaochu Wang, Rashid Aman, Ahmed Mahas, Magdy M Mahfouzand incubated with T4 DNA Ligase (Promega) in 1× T4 DNA Ligase Reaction Buffer (Promega; catalog # M1801) for 3 h at 23°C, followed by 2 h at 16 and 4°C until transformation of E. coli competent cells (Thermo Scientific

(2) Production and Characterization of Novel Fabs Generated from Different Phage Display Libraries as Probes for Immunoassays for Gluten Detection in FoodFoodsAugust 31, 2023Eduardo Garcia-Calvo, Aina García-García, Santiago Rodríguez, Kristiina Takkinen, Rosario Martín, Teresa García#R3189), purification by gel electrophoresis, and ligation with T4 ligase (Promega©, Madison, WI, USA ref #M1801) overnight at 16 °C. The resulting ligations were transformed by chemical transformation into E. coli

(3) Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall ArmywormsBIO-PROTOCOLJuly 20, 2023Iram Gull, Georg JanderPspOMI, SbfI (NEB Biolabs, catalog number: R0653S and R3642)T4 DNA ligase (Promega, catalog number: M1804)100 bp DNA ladder (Promega, catalog number: G210A)Nuclease-free waterAbsolute ethanol (Sigma-Aldrich,

(4) Double DAP-seq uncovered synergistic DNA binding of interacting bZIP transcription factorsNature CommunicationsMay 5, 2023Miaomiao Li, Tao Yao, Wanru Lin, Will E. Hinckley, Mary Galli, Wellington Muchero, Andrea Gallavotti, Jin-Gui Chen, Shao-shan Carol Huangμl 30 μM annealed adapters were ligated in a 50 μl ligation reaction using T4 DNA Ligase (Promega, M1804) incubated at room temperature for 3 h. The DNA was precipitated and suspended in 30 μl Elution Buffer

(5) The Mediator complex subunit MED25 interacts with HDA9 and PIF4 to regulate thermomorphogenesisPlant physiologyMay 2, 2023Shapulatov U, van Zanten M, van Hoogdalem M, Meisenburg M, van Hall A, Kappers I, Fasano C, Facella P, Loh CC, Perrella G, et al.sites (Supplemental Table 4). The expected PCR products were digested and ligated with T4 DNA ligase (#M1801, Promega, Madison, USA) into pIV1A2.1 entry vector (Plant Research International, Wageningen, Netherlands)

(6) Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinomaClinical epigeneticsApril 29, 2023Sgro A, Cursons J, Waryah C, Woodward EA, Foroutan M, Lyu R, Yeoh GCT, Leedman PJ, Blancafort P.(IDT, Integrated DNA Technologies) were annealed and ligated, using T4 DNA ligase (Promega, Cat. No. M1801), into the BbsI recognition site of the transient pUC19 sgRNA (MS2) cloning backbone or into the BsmBI

(7) Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1Journal of Chemical Information and ModelingApril 10, 2023Alessandro Nicoli, Franziska Haag, Patrick Marcinek, Ruiming He, Johanna Kreißl, Jörg Stein, Alessandro Marchetto, Andreas Dunkel, Thomas Hofmann, Dietmar Krautwurst, et al.end (72 °C, 7 min). Insertion of nucleotides into expression vectors was done with T4-DNA ligase (#M1804, Promega, Madison, USA) via EcoRI/NotI (#R6017/#R6435, Promega, Madison, USA) into the expression plasmid

(8) Phase separation of an actin nucleator by junctional microtubules regulates epithelial functionScience advancesFebruary 15, 2023Tsukita K, Kitamata M, Kashihara H, Yano T, Fujiwara I, Day TF, Katsuno T, Kim J, Takenaga F, Tanaka H, et al.(New England Biolabs, Ipswich, MA, USA; catalog no. R0539) and T4 DNA ligase (Promega, Madison, WI, USA; catalog no. M1801) to generate pX330-U6-chimeric-CoblKO1 and pX330-U6-chimeric-CoblKO2 plasmids. Eph4 cells were transfected

(9) Transcriptome-wide m6A methylation in natural yellow leaf of Catalpa fargesiiFrontiers in plant scienceJanuary 1, 2023Zhang Y, Wang J, Ma W, Lu N, Fu P, Yang Y, Zhao L, Hu J, Qu G, Wang N.target gene fragment and pTRV2 were digested by XbaI and SacI and then ligated by T4 ligase (Promega, M1801, China). The specific vector primers were used to detect the recombinant plasmid pTRV2-CfALKBH5 by PCR

(10) Implications of differential transcription start site selection on chronic myeloid leukemia and prostate cancer cell protein expressioniScienceDecember 22, 2022Arif A. Surani, Keith A. Spriggs, Christoph Ufer, Christos Polytarchou, Cristina Montiel-Duartepolymerase,Promega,Cat#M7841 Alkaline Calf Intestinal Phosphatase,Promega,Cat# M1821 T4 DNA Ligase,Promega,Cat#M1801 TRIzol Reagent,ThermoFisher,Cat#15596026 LiCl Precipitation Solution,ThermoFisher,Cat#AM9480 Chloroform,Sigma-Aldrich,Cat#C2432 30%

(11) Interference of flagellar rotation up-regulates the expression of small RNA contributing to Bordetella pertussis infectionScience AdvancesDecember 21, 2022Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Mayuko Osada-Oka, Daisuke Nakane, Katsumi Imada, Yasuhiko Horiguchi69504) and digested with Sau 3AI. The digested products were circulated with T4 DNA ligase (Promega, M1801) and used as a template for PCR with primers TcR-S and Mari5. The PCR products were subjected to direct

(12) Vasa nucleates asymmetric translation along the mitotic spindle during unequal cell divisionsNature CommunicationsDecember 1, 2022Ana Fernandez-Nicolas, Alicia Uchida, Jessica Poon, Mamiko YajimaORF, gel purifying the resulting pSP64-Membrane-mCherry fragment, and re-ligating with T4 DNA ligase (#M1801, Promega, USA) according to the Promega protocol. Please also see Table 1 for a list of all resources

(13) Deletion of the primase-polymerases encoding gene, located in a mobile element in Thermus thermophilus HB27, leads to loss of function mutation of addAB genesFrontiers in MicrobiologyDecember 1, 2022Carlos Verdú, Patricia Pérez-Arnaiz, Ana Peropadre, José Berenguer, Mario MencíaWizard® SV Gel and PCR Clean-Up System (Promega; Ref: A9281) and ligated with T4 DNA ligase (Promega; Ref: M1801). After ligation, reactions were transformed in chemically competent E. coli DH5α and plated in LB –

(14) The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogasteriScienceNovember 18, 2022Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik HedenstrÃ¶m, BjÃ¶rn Bohman, Marie Bengtsson, Paul G. Becher, et al.polymerase chain reaction (PCR), using gene-specific primers (Table S2), ligated with T4-DNA ligase (#M1804, Promega, Madison, USA) either MfeI/NotI (#R3589S/ # R0189S, New England Biolabs, Ipswich, UK) or EcoRI/NotI

(15) Nuclear stabilization of p53 requires a functional nucleolar surveillance pathwayCell RepNovember 1, 2022Katherine M. Hannan, Priscilla Soo, Mei S. Wong, Justine K. Lee, Nadine Hein, Perlita Poh, Kira D. Wysoke, Tobias D. Williams, Christian Montellese, Lorey K. Smith, et al.Elmer,Cat# TGRT53 Nucleospin Plasmid Kit,Macherey-Nagel,Cat# 740588.50 T4 DNA Ligase Kit,Promega,Cat# M1801 NucleoSpin RNA extraction kit,Macherey-Nagel,Cat# 740955.50 RNeasy Mini kit,Qiagen,Cat# 74104 Isolate II RNA mini kit

(16) Pro-Apoptotic and Anti-Invasive Properties Underscore the Tumor-Suppressing Impact of Myoglobin on a Subset of Human Breast Cancer CellsInternational journal of molecular sciencesSeptember 29, 2022Aboouf MA, Armbruster J, Thiersch M, Guscetti F, Kristiansen G, Schraml P, Bicker A, Petry R, Hankeln T, Gassmann M, et al.break. sgRNA oligonucleotides were ligated to plasmids using T4 DNA ligase (Promega, Madison, WI, USA, #M1801) and BbsI restriction enzymes (NEB, Ipswich, MA, USA, #R0539L) according to the manufacturer. Ligated

(17) Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cellsSTAR ProtocolsSeptember 16, 2022Hyebeen Hong, Yoontae Leerestriction enzyme,Enzynomics,R002S XhoI restriction enzyme,Enzynomics,R007S T4 DNA Ligase,Promega,M1801 T4 DNA Ligase 10× buffer,Promega,C1263 Agar,Sigma-Aldrich,A6686 LB Broth Miller,BD Difco,244620 Ampicillin,Sigma-Aldrich,A9393 SacII

(18) The automated Galaxy-SynBioCAD pipeline for synthetic biology design and engineeringNature CommunicationsAugust 29, 2022Joan HÃ©risson, Thomas Duigou, Melchior du Lac, Kenza Bazi-Kabbaj, Mahnaz Sabeti Azad, Gizem Buldum, Olivier Telle, Yorgo El Moubayed, Pablo Carbonell, Neil Swainston, et al.ligase buffer, 1 µL NEB BsaI-HF v2 (NEB #R3733), 1 µL T4 DNA Ligase (NEB M0202 (Micalis), or Promega M1804 (Imperial)) per 20 µL required for each reaction. The Opentrons OT-2 pipetted the 20 µL master mix for

(19) CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in riceThe Plant JournalJuly 9, 2022Mathieu Gonin, Kwanho Jeong, Yoan Coudert, Jeremy Lavarenne, Giang Thi Hoang, Martine Bes, Huong Thi Mai To, Marieâ€Rose Ndella Thiaw, Toan Van Do, Daniel Moukouanga, et al.pro35S‐CRL1‐Tnos and the pro35S‐AS2‐Tnos cassettes were ligated with the open pB2KSP using T4 DNA ligase (Promega M1801, Promega, Madison, WI, USA) and transferred for multiplication into E. coli strain TOP10 (Invitrogen

(20) Native RNA G quadruplex immunoprecipitation (rG4IP) from mammalian cellsSTAR ProtocolsJune 1, 2022Arif A. Surani, Cristina Montiel-DuartePhosphatase,Promega,Cat# M1821 Ampicillin sodium salt,Sigma-Aldrich,Cat#A9518 T4 DNA Ligase,Promega,Cat#M1801 RNasin Ribonuclease Inhibitor,Promega,Cat#N2511 ,, Critical commercial assays,Critical commercial

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T4 DNA Ligase

T4 DNA Ligase from Promega

Description

Specifications/Features

Promega

Citations (84)

Reviews

T4 DNA Ligase from Promega

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