Millicell® Cell Culture Insert, 12 mm, HA mixed cellulose esters, 0.45 µm from MilliporeSigma

Product Specs
ItemMillicell® Cell Culture Insert, 12 mm, HA mixed cellulose esters, 0.45 µm
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPIHA01250
Quantity50
Brand/TrademarkMillicell

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Maternal vitamin B1 is a determinant for the fate of primordial follicle formation in offspringNature CommunicationsNovember 16, 2023Wen-Xiang Liu, Hai-Ning Liu, Zhan-Ping Weng, Qi Geng, Yue Zhang, Ya-Feng Li, Wei Shen, Yang Zhou, Teng ZhangAfter isolation ovaries from newborn mice, the ovaries were cultured on 3D-culture membrane (Millipore, PIHA01250) for 72-h. The culture medium was DMEM/F12 (Gibco, C11330500BT, Beijing, China) supplemented with 10%

(2) Preparation of biological monolayers for producing high-resolution scanning electron micrographsPLOS ONEJuly 8, 2022Shireen Mentor, Franscious Cummings, David Fisher5x105 cells/insert on a Millicell, mixed cellulose esters insert membrane (Millipore/Merck, Cat no. PIHA01250, Germany). After exposure of the cell monolayers to the standard culture medium (i.e. supplemented DMEM:F12),

(3) Bordetella pertussis Induces Interferon Gamma Production by Natural Killer Cells, Resulting in Chemoattraction by Respiratory Epithelial CellsThe Journal of Infectious DiseasesApril 1, 2022Gerco den Hartog, Marcel A Schijf, Guy A M Berbers, Fiona R M van der Klis, Anne-Marie Buismansodium pyruvate, and 10% fetal bovine serum (FBS); 50 000 cells were added to cell culture inserts (PIHA01250, Millipore) and allowed to form confluent monolayers in submerged cultures. PBMCs and NK cells were

(4) The Effect of Normoxic and Hypoxic U-87 Glioblastoma Paracrine Secretion on the Modulation of Brain Endothelial CellsCellsJanuary 14, 2022Mariam Rado, Brian Flepisi, David FisherThis allowed for U-87 cells to be cultured in wells, while a three-legged insert (Merck–Millipore, PIHA01250, 6 Hatters Ln, Watford, UK) was used for growing the brain endothelial cell monolayers. These three-legged

(5) The Role of Cytoskeletal Proteins in the Formation of a Functional In Vitro Blood-Brain Barrier ModelInternational journal of molecular sciencesJanuary 11, 2022Mentor S, Makhathini KB, Fisher D.of 24 h after seeding, 5 × 105 bEnd5 cells/insert/well on membrane inserts (Millipore/Merck, Cat no. PIHA01250, Darmstadt, Germany), in 24-well plates until they reached confluence and the cultured medium was replaced

(6) ELF3 Mediates IL-1α Induced Differentiation of Mesenchymal Stem Cells to Inflammatory iCAFsStem CellsDecember 1, 2021Linda L. Tran, Truong Dang, Rintu Thomas, David R. Rowleywere seeded into the upper chamber of 12 mm Millicell Cell Culture Inserts (HA mixed cellulose esters, PIHA01250, Millipore) in separate plates in their own respective mediums (listed above), as we have reported previously

(7) Differential Effects of Normoxic versus Hypoxic Derived Breast Cancer Paracrine Factors on Brain Endothelial CellsBiology (Basel)November 27, 2021Mariam Rado, Brian Flepisi, David Fisherof 0.45μm, filtration diameter of 12 mm, and an effective surface area of 0.6 cm2) (Merck-Millipore. PIHA01250, 6 Hatters Ln, Watford, UK) were treated by adding MCF7CM to the apical insert chamber on a daily basis.

(8) High-Resolution Insights Into the in vitro Developing Blood-Brain Barrier: Novel Morphological Features of Endothelial Nanotube FunctionFrontiers in NeuroanatomyJune 25, 2021Shireen Mentor, David Fishercellulose esters, according to the manufacturers’ specifications (Millicell® insert, (Merck), Cat no. PIHA01250). Inserts were placed in 24-well tissue culture microtiter plates (Adcock Ingram). BEC monolayers (b.End5)

(9) Apurinic/Apyrimidinic Endonuclease 1 Restricts the Internalization of Bacteria Into Human Intestinal Epithelial Cells Through the Inhibition of Rac1Frontiers in ImmunologyFebruary 2, 2021Gerco den Hartog et al.expression was substantially Barrier Studies 500,000 T84 cells were seeded onto inserts (Millipore PIHA01250) and allowed to develop trans-epithelial electrical resistance (TEER of >1,000 W*cm2) (34). Trans-epithelial electrical

(10) Detrimental effect of Bisphenol S in mouse germ cell cyst breakdown and primordial follicle assemblyChemosphereFebruary 1, 2021Wen-Xiang Liu, Farini Donatella, Shao-Jing Tan, Wei Ge, Jun-Jie Wang, Xiao-Feng Sun, Shun-Feng Cheng, Wei Shen2018). Brieﬂy, after isolation, the ovaries were cultured on Millicell-PC membrane inserts (Millipore, PIHA01250, Medford, MA, USA) for 3 days. The culture medium was Dulbecco’s Modiﬁed Eagle’s Medium (DMEM)/F12 (Hyclone,

(11) Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor IdentityDevelopmental CellJanuary 1, 2019Jing Liu et al.oxidized",Sigma,7154 5-Azacytidine,Sigma,A2385 Paraformaldehyde,Sigma,P6148 Millicell,Millipore,PIHA01250 Trypsin,Sigma,C5138-5G SuperScript III ReverseTranscriptase,Thermo,18080085 RNaseOUT RecombinantRibonuclease

(12) Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesisAgingMarch 28, 2018Wang YF, Sun XF, Han ZL, Li L, Ge W, Zhao Y, De Felici M, Shen W, Cheng SF.described previously [64]. Briefly, ovaries were collected and cultured on Millicell-PC membrane inserts (Millipore, PIHA01250, Medford, MA, USA) with medium filling only the lower chamber for 5 days (equal to 4 dpp); the medium

(13) Local anaesthetics or their combination with morphine and/or magnesium sulphate are toxic for equine chondrocytes and synoviocytes in vitroBMC Veterinary ResearchDecember 1, 2017L. M. Rubio-Martínez, E. Rioja, M. Castro Martins, S. Wipawee, P. Clegg, M. J. Peffersequilibration at 37 °C, 5% O2 for 24 h, trans-well inserts (0.45 μm pore, 12 mm; Millicell Cell Culture Inserts PIHA01250; Sigma-Aldrich, Darmstadt, Germany) were located into the wells and cartilage explants from 7 horses

(14) An In Vitro System to Study Sertoli Cell Blood-Testis Barrier DynamicsMethods in Molecular BiologyJuly 29, 2014Dolores D. Mruk, C. Yan ChengFunction 2.2.1. Materials Millicell-HA culture plate inserts (Millipore, Billerica, MA) (e.g., Cat. #: PIHA01250, 12-mm diameter; ~1.1304 cm2 surface area). Millicell ERS system (Millipore, Cat. #:MERS 000 01): meter

(15) Survival of Mature CD4 T Lymphocytes Is Dependent on Major Histocompatibility Complex Class II–expressing Dendritic CellsJ. Exp. MedOctober 20, 1997Thomas Brockercounted as day 0 of pregnancy). The thymic lobes were placed on culture plate inserts (Millipore No. PIHA01250; Bedford, MA) floating on culture medium containing 1.35 mM 2′-deoxyguanosine (dGuo; No. D-0901; Sigma

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