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Protein A Agarose Beads
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Protein A Agarose Beads
Protein A Agarose Beads
Protein A Agarose Beads from Cell Signaling Technology
Be the first to write a review!
Citations:
(323)
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Cell Signaling Technology for
Protein A Agarose Beads
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Reviews
Product Specs
Item
Protein A Agarose Beads
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
9863S
Quantity
1 ml
Applications
IP
Research Area
Related Products
Type
Resins
Mode
Antibody Purification
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (323)
(1)
Zika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathways
Nat Commun
December 5, 2023
Solène Denolly, Alexey Stukalov, Uladzimir Barayeu, Alina N. Rosinski, Paraskevi Kritsiligkou, Sebastian Joecks, Tobias P. Dick, Andreas Pichlmair, Ralf Bartenschlager
mM Tris-HCl [pH 7.4], 150 mM NaCl, 0.1% NP-40 and 10U RNase inhibitor), Samples were incubated with
protein A agarose beads
at 4 °C for 2 h. After 3 washes with IP buffer, bound RNAs were eluted with 2×100 uL of elution buffer
(2)
Spinal microRNA-134-5p targets glutamate receptor ionotropic kainate 3 to modulate opioid induced hyperalgesia in mice
Molecular Pain
December 1, 2023
Zhen Wang, Yao Yao, Yuzhu Tao, Peixin Fan, Yonghao Yu, Keliang Xie, Guolin Wang
Argonaute-2 antibodies (
1:50
,
Cell Signaling Technology
C34C6) at 4°C overnight. On the following day,
protein A agarose beads
(Santa Cruz Biotechnology) were added, mixed, incubated for 1 h at 4°C, and then centrifuged. The supernatant
(3)
The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells
BMC Cancer
November 25, 2023
Celimene Galiger, Fatema Tuj Zohora, Carmen Dorneburg, Daniel Tews, Klaus-Michael Debatin, Christian Beltinger
(10,508–1-AP, Proteintech) and 2–4 µg rabbit IgG (
2729
,
Cell Signaling Technology
), and with 30 μl of
protein A agarose beads
(
16–125
,
Merck Millipore
) overnight at 4 °C.
For Western blot
analysis of OXPHOS and glycolysis proteins
(4)
MIWI N-terminal RG motif promotes efficient pachytene piRNA production and spermatogenesis independent of LINE1 transposon silencing
PLOS Genetics
November 13, 2023
Chao Wei, Jiongjie Jing, Xiaoyuan Yan, Jeffrey M. Mann, Ruirong Geng, Huirong Xie, Elena Y. Demireva, Rex A. Hess, Deqiang Ding, Chen Chen
4°C. Anti-MILI (
PM044
,
MBL
) or anti-MIWI (
2079
,
Cell Signaling Technology
) antibodies together with
protein-A agarose beads
were added to the lysates and incubated for 4 h at 4°C. The beads were washed in lysis buffer 5 times.
(5)
SUMOylation indirectly suppresses activity of the HIF-1α pathway in intestinal epithelial cells
Journal of Biological Chemistry
November 1, 2023
Mykyta I. Malkov, Darragh Flood, Cormac T. Taylor
isolate the antibody-bound proteins, 20 μl of
Protein A Agarose Beads
(
Cell Signaling
; catalog no.:
9863
) were added to each sample and incubated for 4 h at 4 °C with end-to-end rotation. Agarose beads were
(6)
Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling
Bone research
October 26, 2023
Liu RX, Gu RH, Li ZP, Hao ZQ, Hu QX, Li ZY, Wang XG, Tang W, Wang XH, Zeng YK,
et al
.
protease inhibitors
. After
incubation
on ice for 30 min, 40 μL of 50% protein A agarose bead slurry (
CST
, Cat#
9863
, USA) was used to preclear cell extracts for 30 min at 4 °C, followed by
incubation
with antibodies
(7)
Single-cell signaling analysis reveals that Major Vault Protein facilitates RasG12C inhibitor resistance
bioRxiv : the preprint server for biology
October 4, 2023
Zhang JZ, Ong SE, Baker D, Maly DJ.
Fisher,PI26620
Spectra™ Multicolor High Range Protein Ladder
,
Thermo Fisher
,
26625
Protein A Agarose Beads
,
CST
,
9863S
Protein G Agarose Beads
,
CST
,
37478
Puromycin,Tocris,4089 Hygromycin,Tocris,4137 DPBS,
Gibco
,
14190250
Doxycycline,Tocris,4090 PEI
(8)
Raptor mediates the selective inhibitory effect of cardamonin on RRAGC-mutant B cell lymphoma
BMC Complementary Medicine and Therapies
September 26, 2023
Ying Liu, Yanting Zhu, Huajiao Chen, Jintuo Zhou, Peiguang Niu, Daohua Shi
HRP-labeled anti-
mouse
(#
7076
) and anti-rabbit (#
7074
) secondary antibody,
Protein A Agarose Beads
(#
9863
), Rabbit IgG Isotype Control (#3900) were from
Cell Signaling Technology
(Danvers, MA, USA). Cardamonin
(9)
Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia
Clinical Cancer Research
August 15, 2023
Caner Saygin, Giorgia Giordano, Kathryn Shimamoto, Bart Eisfelder, Anika Thomas-Toth, Girish Venkataraman, Vijayalakshmi Ananthanarayanan, Tiffaney L. Vincent, Adam DuVall, Anand A. Patel,
et al.
and incubated with rotation for 2 hours at 4°C.
Protein A agarose beads
(
Cell Signaling Technology
, #
9863
) were added to lysates (10–30 μL of 50% bead slurry), which were subsequently incubated with rotation
(10)
Mitotic phosphorylation of Tau/MAPT modulates cell cycle progression in prostate cancer cells
Journal of Cancer Research and Clinical Oncology
August 1, 2023
Letizia Clementi, Samantha Sabetta, Veronica Zelli, Chiara Compagnoni, Alessandra Tessitore, Vincenzo Mattei, Adriano Angelucci
Technology) was incubated for 2 h at 4 °C with
protein-G Agarose Beads
(
Cell Signalling
Technology) and
Protein A-Agarose Beads
(Santa Cruz Biotechnology) in Tris 50 mM pH 7.4, NP40 1%, Na-deoxycholate 0.25%, NaCl 150 mM and EDTA
(11)
LAR Downregulation Protects the Astrocytic U251 and Cocultured SH-SY5Y Cells in a Rotenone-Induced Parkinson’s Disease Cell Model
International Journal of Molecular Sciences
July 5, 2023
Wei Zheng, Xiao Han, Bing Han, Gang Li, Jing Gan, Tian Wang, Bo Xu, Jie He, Wenxiao Du, Xiaolin Cao,
et al.
protein using anti-IGF-1Rβ (
Cell Signaling Technology
, Danvers, MA, USA) followed by
incubation
with
protein A agarose beads
(Absin, Shanghai, China). Samples were separated via 5%, 6%, 10%, 12%, or 15% gels
SDS-PAGE
(Mini-PROTEAN,
(12)
AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity
Journal of Biological Chemistry
June 1, 2023
Mario Ruiz, Ranjan Devkota, Delaney Kaper, Hanna Ruhanen, Kiran Busayavalasa, Uroš Radović, Marcus Henricsson, Reijo Käkelä, Jan Borén, Marc Pilon
monoclonal anti-HA antibody, 7 μl (C29F4,
Cell signaling
, RRID: AB_1549585, Cat#
3724
) and 20 μl of
protein A-agarose beads
(Roche) were added to the lysate, which was then rotated at 4 °C for 1 h (samples for
mass spectroscopy
).
(13)
Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assembly
Journal of Cancer
May 21, 2023
Jintian Song, Yigui Chen, Hui Yu, Liang Zheng, Yi Wang, Dan Li
inhibitor
(
36978
,
Thermo
, USA). Subsequently, extracted protein and 50 mL
Protein A Agarose Beads
(
9863
,
CST
, USA) were fully reacted for 4 h at room temperature, and then the admixture was incubated with
(14)
Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling
Molecular Cell
May 18, 2023
Edoardo José Longarini, Helen Dauben, Carolina Locatelli, Anne R. Wondisford, Rebecca Smith, Charlotte Muench, Andreas Kolvenbach, Michelle Lee Lynskey, Alexis Pope, Juan José Bonfiglio,
et al.
65601
Chromotek GFP-trap Magnetic Particles M-270,Chromotek,Cat# gtd
Protein A Agarose Beads
,
Cell Signaling
,Cat#
9863
"Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well",Themo Scientific,Cat#
15117
Guide-it
CRISPR/Cas9
(15)
Inhibition of ERK signaling for treatment of ERRα positive TNBC
PLOS ONE
May 10, 2023
David Musheyev, Esther Miller, Natania Birnbaum, Elisheva Miller, Shoshana Erblich, Alyssa Schuck, Anya Alayev
either Sepharose®Bead Conjugated
p44/42 MAPK (Erk1/2) antibody
#
5736
(
Cell Signaling Technology
) or
Protein A Agarose beads
, 50% slurry (EMD Millipore) overnight at 4°C. Beads were pelleted from solution, washed twice with IP
(16)
Driver Mutations Dictate the Immunologic Landscape and Response to Checkpoint Immunotherapy of Glioblastoma
Cancer Immunology Research
May 3, 2023
Alan T. Yeo, Rushil Shah, Konstantinos Aliazis, Rinku Pal, Tuoye Xu, Piyan Zhang, Shruti Rawal, Christopher M. Rose, Frederick S. Varn, Vicky A. Appleman,
et al.
NaCl). A phospho-tyrosine specific antibody (P-Tyr-1000,
Cell Signaling Technology
) was incubated with
protein A agarose beads
(Roche) overnight at 4°C in 1% PBS to bind the antibody to the beads. Subsequently, the antibody-bead
(17)
RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancer
World Journal of Surgical Oncology
April 14, 2023
Yan Hu, Hanshuo Mu, Zhiping Deng
10 min, the cell lysates were incubated with indicated antibody and protein A/G PLUS agarose beads (#
9863
, #
37478
,
Cell Signaling Technology
) overnight at 4 °C. After washing, the beads were lysed with loading
(18)
CDK9 inhibition induces epigenetic reprogramming revealing strategies to circumvent resistance in lymphoma
Molecular Cancer
March 30, 2023
Elana Thieme, Nur Bruss, Duanchen Sun, Edward C. Dominguez, Daniel Coleman, Tingting Liu, Carly Roleder, Melissa Martinez, Krystine Garcia-Mansfield, Brian Ball,
et al.
pre-cleared and incubated at 4 °C overnight with 2 µg of BRD4 antibody or rabbit IgG isotype control.
Protein A agarose beads
(20 µL,
Cell Signaling Technology
) were added to lysates and samples were incubated for 1.5 h at 4 °C.
(19)
Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70
Biomedicines
March 12, 2023
Suyama K, Sakai D, Hayashi S, Qu N, Terayama H, Kiyoshima D, Nagahori K, Watanabe M.
Germany) and PhosSTOPTM (Roche).
Immunoprecipitation
was performed using
Protein A Agarose Beads
(#
9863
;
Cell Signaling Technology
) following a standard protocol. Proteins were separated by sodium dodecyl
(20)
Linear ubiquitination of LKB1 activates AMPK pathway to inhibit NLRP3 inflammasome response and reduce chondrocyte pyroptosis in osteoarthritis
Journal of orthopaedic translation
March 1, 2023
Chen Y, Liu Y, Jiang K, Wen Z, Cao X, Wu S.
Proteintech
, USA) at 4 °C overnight. Cell lysates were then combined with protein A/G agarose beads (#
9863
,
Cell Signaling Technology
, USA) for 2 h. Subsequently, collected precipitates were washed with ice
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Protein A Agarose Beads from Cell Signaling Technology
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