ScaI-HF from New England Biolabs

Product Specs
ItemScaI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3122S
Quantity1000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

AGT^ACT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Humanized CD36 (hCD36) mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36Experimental AnimalsNovember 9, 2023Xiulong Xie, Zhenlan Niu, Linlin Wang, Xiaofei Zhou, Xingyan Yu, Hongyan Jing, Yi YangG418-resistant ES clones were subjected to Southern Blot assay. We digested cellular DNA with ScaI (R3122S, NEB, Ipswich, MA, USA) or EcoNI (R0521S, NEB) and hybridized using 2 probes, respectively (Restriction enzyme digestion

(2) Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cellsSTAR ProtocolsJune 1, 2023Nannan Kong, Ying Wai Chan,New England Biolabs,Cat# M0318S BamHI-HF,New England Biolabs,Cat# 3136S ScaI-HF,New England Biolabs,Cat# R3122S Quick CIP,New England Biolabs,Cat# M0525S MAX Efficiency™ DH5α Competent Cells,Thermo Fisher,Cat #18258012 GeneJET Gel Extraction Kit

(3) SARS-CoV-2 concentration in wastewater consistently predicts trends in COVID-19 case counts by at least two days across multiple WWTP scalesEnvironmental AdvancesApril 1, 2023Candice L. Swift, Mirza Isanovic, Karlen E. Correa Velez, R. Sean Norman(Integrated DNA Technologies, Cat. No. 10006625) was digested using ScaI-HF (New England Biolabs Cat. No. R3122S) according to the manufacturer's protocol. The digested product was cleaned using SPRIselect beads (Beckman

(4) Direct wastewater extraction as a simple and effective method for SARS-CoV-2 surveillance and COVID-19 community-level monitoringFEMS MicrobesJanuary 17, 2023Megan E J Lott, William A Norfolk, Cody A Dailey, Amelia M Foley, Carolina Melendez-Declet, Megan J Robertson, Stephen L Rathbun, Erin K Lippand N2 genes, was linearized by enzymatic digestion with ScaI-HF (New England Biolabs, catalog number R3122S). Briefly, 10 µl of the plasmid was incubated with 5 U of ScaI-HF in a 50 µl reaction at 37°C for 60

(5) Temperature dependent in vitro binding and release of target DNA by Cas9 enzymeScientific ReportsSeptember 9, 2022Serene Rose David, Sumanth Kumar Maheshwaram, Divya Shet, Mahesh B. Lakshminarayana, Gautam V. Soniplasmid were generated by nicking the plasmid with Nt.BspQ1 (NEB, R0644S) and digesting with ScaI (NEB, R3122S) or NotI (NEB R0189S) enzymes, respectively. The target sequence on the plasmid used in this study is:

(6) SARS-CoV-2 concentrations in a wastewater collection system indicated potential COVID-19 hotspots at the zip code levelScience of The Total EnvironmentDecember 15, 2021Renys E. Barrios, Chin Lim, Megan S. Kelley, Xu Li(PTC) plasmid (IDT # 10006625) was linearized using the ScaI High Fidelity (HF) restriction enzyme (R3122S, 1000 units, New England BioLabs Inc., Ipswich, MA) and then cleaned using the Monarch PCR and DNA cleanup

(7) Electrochemical Strategy for Low-Cost Viral DetectionACS Central ScienceJune 23, 2021Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperichplasmids containing an EcoRV sites flanking the HPV 18 E7 sequence were linearized with ScaI-HF (NEB, R3122S) and AatII (NEB, R0117S). HIV plasmid preparation can be found in the Materials and Methods section

(8) A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoirCell Reports MedicineApril 20, 2021Claire N. Levy et al.,New England BioLabs,Cat#R0656S PmeI,New England BioLabs,Cat#R0560S HindIII,New England BioLabs,Cat#R0104S ScaI,New England BioLabs,Cat#R3122S BglI,New England BioLabs,Cat#R0143S "Proteinase K, 20mg/mL, > 318mAu/mL at 30°C",QIAGEN,Cat#19131 AatII,New England BioLabs

(9) Clustering of strong replicators associated with active promoters is sufficient to establish an early‐replicating domainThe EMBO JournalNovember 2, 2020Caroline Brossas et al.entry vectors, with LR clonase. For electroporation, the final vector was linearized with ScaI (NEB #R3122S). We used four entry vectors to generate the β A ‐globin + β‐actin construct inserted at site 3. We

(10) Systemic AAV vectors for widespread and targeted gene delivery in rodentsNature ProtocolsFebruary 1, 2019Rosemary C. Challis et al.Qubit dsDNA HS assay kit (Invitrogen, cat. no. Q32854) ● ScaI-HF restriction enzyme (New England Biolabs, cat. no. R3122S) or other enzyme that cuts outside of the rAAV genome and within the pAAV backbone ● SYBR green master

(11) Naturally Occurring Off-Switches for CRISPR-Cas9CellDecember 15, 2016April Pawluk et al.,New England Biolabs,#R0146S BsmBI,New England Biolabs,#R0580S BstBI,New England Biolabs,#R0519S ScaI-HF,New England Biolabs,#R3122S Tobacco Etch Virus (TEV) protease,A. Davidson Lab, Ni-NTA agarose resin,Qiagen,#30210 "cOmplete,

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ScaI-HF

ScaI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (11)

Reviews

ScaI-HF from New England Biolabs

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