Description
The Eclipse™ solves many difficult separations, fractionation, and sizing tasks for macromolecules, nanoparticles and colloids by field-flow fractionation (FFF). Combined with Wyatt’s DAWN™; or miniDAWN™; multi-angle light scattering (MALS) detector and OptilabTM, refractive index detector, the Eclipse offers a uniquely versatile FFF-MALS solution providing characterization of absolute molar mass, size, particle concentration, conformation and encapsulation.
The system is used to characterize proteins, polymers, viruses and viral vectors, exosomes, drug- and gene-delivery nanoparticles, environmental tracers, and nano-emulsions. It provides robust separation in 20-60 minutes of macromolecules and nanoparticles from 1 nm - 1000 nm and because it doesn't use a stationary phase there is minimal shear and surface interactions. The system includes a versatile choice of separation channels, permitting sample loads from nanograms to milligrams, including semi-preparative FFF. Separation resolution is optimized simply by adjusting flow ratios, controlled by VISION™ software that provides computer-aided method development to save time and boost productivity.
Optional features include dilution control and automated switching between the FFF channel and size-exclusion column. Additionally, fractions can be collected and used for off-line analysis (e.g. mass spectrometry) or coupled to ICP-MS for elemental analysis.