HaloTag Alexa Fluor 488 Ligand from Promega

Product Specs
ItemHaloTag Alexa Fluor 488 Ligand
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG1001
Quantity30microl

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) HaloTag display enables quantitative single-particle characterization and functionalization of engineered extracellular vesiclesbioRxivOctober 1, 2023Roxana E. Mitrut, Devin M. Stranford, Jonathan M. Chan, Matthew D. Bailey, Minrui Luo, Thomas J. Meade, Muzhou Wang, Joshua N. Leonardotherwise stated. All optical filters were acquired from Semrock. Alexa Fluor 488 HaloTag ligand (Promega, G1001) was excited by a 473-nm laser (Laser Quantum, 500 mW), which was passed through a bandpass filter (LD01-473/10–12.5),

(2) A tetracationic porphyrin with dual anti-prion activityiScienceSeptember 15, 2023Antonio Masone, Chiara Zucchelli, Enrico Caruso, Giada Lavigna, Hasier Eraña, Gabriele Giachin, Laura Tapella, Liliana Comerio, Elena Restelli, Ilaria Raimondi, et al.were incubated for 15 min at 37°C with the HaloTag Alexa Fluor 488 cell-impermeant ligand (Promega G1001) diluted 1:1000 in the culture medium, then rinsed three times with PBS. PrP-Halo fluorescence was analyzed

(3) Characterization of Fibrodysplasia Ossificans Progessiva relevant Acvr1/Acvr2 Activin receptors in medaka (Oryzias latipes)PLOS ONESeptember 14, 2023Michael Trumpp, Wen Hui Tan, Wiktor Burdzinski, Yara Basler, Jerome Jatzlau, Petra Knaus, Christoph Winklerwith PBS and simultaneously incubated with the fluorescent Halo-ligand CA-Alexa Fluor 488 (Promega, #G1002) and 2 nM ActivinA-Cy5 for 30 minutes at 4°C to prevent internalization. Subsequently, cells were washed

(4) N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cellsiScienceJanuary 20, 2023Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizukaproteins","Chemicals, peptides, and recombinant proteins" ,, Halo-tag Alexa 488 ligand,Promega,Cat#G1002 Halo-tag TMR ligand,Promega,Cat#G8251 CFSE,Bay Bioscience,Cat#13-0850-U500 PNGase F,Roche,Cat#11365169001 cOmplete

(5) A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand bindingCommunications BiologyJanuary 12, 2023Jerome Jatzlau, Wiktor Burdzinski, Michael Trumpp, Leon Obendorf, Kilian Roßmann, Katharina Ravn, Marko Hyvönen, Francesca Bottanelli, Johannes Broichhagen, Petra Knauscell-impermeable fluorescent ligands were utilized: HaloTag Fluorescent Ligand Alexa Fluor 488 (Promega, #G1002) in the following abbreviated as CA-Alexa488; SNAP-Surface 594 (NEB, #S9134S) in the following abbreviated

(6) Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subuniteLifeDecember 13, 2022Shoji Tane, Keishi Shintomi, Kazuhisa Kinoshita, Yuko Tsubota, Makoto M Yoshida, Tomoko Nishiyama, Tatsuya Hirano(1/500) Other,Streptavidin,Merck,S4762,1 mg/ml (Loop extrusion assay) Other,HaloTag Alexa Fluor 488 Ligand,Promega,G1001, Other,PD-10 column,Cytiva,17-0851-01, Other,Dynabeads Protein A,Thermo Fisher Scientific,10002D,For

(7) Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptorNature CommunicationsOctober 26, 2022Junke Liu, Hengmin Tang, Chanjuan Xu, Shengnan Zhou, Xunying Zhu, Yuanyuan Li, Laurent PrÃ©zeau, Tao Xu, Jean-Philippe Pin, Philippe Rondard, et al.MO, USA). Coelenterazine-h (S2011), HaloTag® Alexa Fluor®660 (G8471), and HaloTag® Alexa Fluor® 488 (G1001) were purchased from Promega Corporation (Madison, WI, USA). Halo-Lumi4-Tb (SHALOTBC) labeling reagents,

(8) Supramolecular CRISPR-OFF switches with host–guest chemistryNucleic Acids ResearchFebruary 22, 2022Wei Xiong, Xingyu Liu, Qianqian Qi, Huimin Ji, Fengbo Liu, Cheng Zhong, Simin Liu, Tian Tian, Xiang Zhouassay using dCas9 The Halo domain of dCas9 was labeled by incubation with fluorescent Halo ligand (G1002 HaloTag® Alexa Fluor® 488 ligand, Promega) at ratio of 1:10 at room temperature for 30 min, and purified

(9) Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRETCell chemical biologyJanuary 20, 2022Lay CS, Bridges A, Goulding J, Briddon SJ, Soloviev Z, Craggs PD, Hill SJ.impermeant substrate,New England BioLabs,Cat# S9124S AlexaFluor 488 HaloTag Ligand,Promega Corporation,Cat# G1001 "5(6)-TAMRA (5-(and-6)-Carboxytetramethylrhodamine), mixed isomers",ThermoFisher Scientific,Cat# C300 "TAMRA, SE; 5-(and-6)-Carboxytetramethylrhodamine, Succinimidyl Ester (5(6)-TAMRA, SE), mixed isomers

(10) The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophoreAutophagyNovember 2, 2021Hao-Chun Chang, Ran N. Tao, Chong Teik Tan, Ya Jun Wu, Boon Huat Bay, Victor C. YuHalo-LC3 were subjected to different treatment as indicated. The cells were stained with 1 M MIL (Promega, G1001) at 37°C for 15 min. The cells were then fixed with 4% paraformaldehyde at 37°C for 5 min. After wash

(11) Auxiliary Subunits Regulate the Dendritic Turnover of AMPA Receptors in Mouse Hippocampal NeuronsFrontiers in Molecular NeuroscienceAugust 23, 2021Ali Harb, Nils Vogel, Ali Shaib, Ute Becherer, Dieter Bruns, Ralf Mohrmanntransfected neurons were first incubated with the membrane-impermeable Alexa Fluor 488 ligand (Promega, G1001) for 35–40 min (in 5% CO2) at 15°C, in this way attenuating receptor endocytosis during the labeling

(12) Rab14/MACF2 complex regulates endosomal targeting during cytokinesisMolecular biology of the cellApril 1, 2021Gibieža P, Peterman E, Hoffman HK, Van Engeleburg S, Skeberdis VA, Prekeris R.(Sigma-Aldrich, F3165) was cross-linked to Protein G–Sepharose beads. HaloTag Alexa Fluor 488 Ligand (G1002) was purchased from Promega (Madison, WI). RNA isolation and quantitative PCR (qPCR) RNA was isolated

(13) ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formationDevelopmental cellFebruary 22, 2021Miao G, Zhao H, Li Y, Ji M, Chen Y, Shi Y, Bi Y, Wang P, Zhang H.Then cells were incubated with membrane-impermeable ligand (MIL) HaloTag® Alexa Fluor® 488 ligand (G1001, Promega) at 37°C for 15 min. Cells were fixed in 4% PFA for 20 min at room temperature, washed 3 times

(14) Sulfated glycans engage the Ang-Tie pathway to regulate vascular developmentNature chemical biologyFebruary 1, 2021Griffin ME, Sorum AW, Miller GM, Goddard WA 3rd, Hsieh-Wilson LC.confluency. Cells were then treated with 1:1,000 cell-impermeable Alexa Fluor 488-conjugated CL (AF488-CL, G1001, Promega) and 1:1,000 Hoescht 33342 (H3750, ThermoFisher Scientific) in complete DMEM at 37 °C for 15

(15) A versatile system to record cell-cell interactionseLifeOctober 7, 2020Tang R, Murray CW, Linde IL, Kramer NJ, Lyu Z, Tsai MK, Chen LC, Cai H, Gitler AD, Engleman E, et al.1.00E+07 cells/mL on ice for 5 min in the presence of either 1 µM HaloTag-Alexa Fluor 488 (Promega G1001) or 3.5 µM HaloTag-Alexa Fluor 660 (Promega G8471). Stained sender cells were then washed twice in Cell

(16) Molecular mechanism for direct actin force-sensing by α-catenineLifeSeptember 24, 2020Mei L, Espinosa de Los Reyes S, Reynolds MJ, Leicher R, Liu S, Alushin GM.for the fluorescent dye JF-646-Halo "Chemical compound, drug",HaloTag Alexa Fluor 488 Ligand,Promega,G1001, "Chemical compound, drug",Alexa Fluor 555-Phalloidin,ThermoFisher,A34055, Biological sample (O. cuniculus),Actin Protein (Rhodamine): Rabbit Skeletal Muscle

(17) Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapyNature cell biologySeptember 1, 2020Gao Y, Nihira NT, Bu X, Chu C, Zhang J, Kolodziejczyk A, Fan Y, Chan NT, Ma L, Liu J, et al.with DAPI. For Halo-tag imaging53, cells were treated with 0.5 μM HaloTag Alexa Fluor 488 Ligand (#G1002, Promega) for 15 min. Images were captured using confocal microscopy (Nikon Ti inverted or Zeiss LSM880)

(18) A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scissionNature structural & molecular biologyJune 1, 2020Flower TG, Takahashi Y, Hudait A, Rose K, Tjahjono N, Pak AJ, Yokom AL, Liang X, Wang HG, Bouamr F, et al.(Invitrogen, NucBlue, R37605); Membrane-impermeable HaloTag Ligand (MIL) (Promega, Alexa Fluor 488-conjugated, G1001); Membrane-permeable HaloTag Ligand (MPL) (Promega, tetramethylrhodamine-conjugated, G8251); normal

(19) Simultaneous tracking of two motor domains reveals near simultaneous steps and stutter steps of myosin 10 on actin filament bundlesBiochemical and Biophysical Research CommunicationsApril 1, 2020Xianan Qin et al.0.025 ADP, 0.5 EGTA and 50% Glycerol (pH adjusted to 7.60). Alexa Fluor 488 (Alexa 488) HaloTag ligand (G1001, Promega) was conjugated to the HaloTag fused to the N-terminus of these purified Myo10 with mApple

(20) Autophagy contributes to BMP type 2 receptor degradation and development of pulmonary arterial hypertensionThe Journal of PathologyNovember 1, 2019Maria Catalina Gomez‐Puerto et al.Next, they were incubated in MCDB131 media containing the non‐permeable HaloTag Alexa Fluor 488 ligand (G1001; Promega) at a dilution of 1:10 × 103 for 15 min at 37°C. Cells were then washed three times with medium

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