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Polymerase Chain Reaction (PCR)
Primers and Probes
Oligo d(T)23VN
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Oligo d(T)23VN
Oligo d(T)23VN from New England Biolabs
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Citations:
(9)
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Oligo d(T)23VN
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Description
Oligo d(T)23 VN is used for the priming and sequencing of mRNA adjacent to the 3'-poly A tail (1).
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Oligo d(T)23VN
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
S1327S
Target
Oligo d(T)23VN
Quantity
1 a260 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Primer Type
Oligo dT Primers
Type
Oligo
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (9)
(1)
CRISPR-Cas9 engineering of the RAG2 locus via complete coding sequence replacement for therapeutic applications
Nature Communications
October 27, 2023
Daniel Allen, Orli Knop, Bryan Itkowitz, Nechama Kalter, Michael Rosenberg, Ortal Iancu, Katia Beider, Yu Nee Lee, Arnon Nagler, Raz Somech,
et al.
T cells
obtained on day 28 of IVTD. cDNA preparation was executed from RNA, using Oligo d(T)23 VN-
S1327S
(
NEB
), dNTPs 10 mM (Sigma-Aldrich), and
M-MuLV Reverse Transcriptase
(
NEB
).
qRT-PCR
reactions were conducted
(2)
Molecular mechanisms of stress-induced reactivation in mumps virus condensates
Cell
April 27, 2023
Xiaojie Zhang, Sindhuja Sridharan, Ievgeniia Zagoriy, Christina Eugster Oegema, Cyan Ching, Tim Pflaesterer, Herman K.H. Fung, Isabelle Becher, Ina Poser, Christoph W. Müller,
et al.
ProLong Gold Antifade Reagent with DAPI
,
Cell Signaling Technology
,Cat#
8961
Oligo d(T)23 primer,
New England Biolabs
,Cat#
S1327S
EDTA-free protease
inhibitor
cocktail,
Roche
,04693132001
PhosSTOP
Phosphatase inhibitor cocktail,
Roche
,
4906845001
"Benzonase
(3)
FIPRESCI: droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencing
Genome Biology
April 6, 2023
Yun Li, Zheng Huang, Zhaojun Zhang, Qifei Wang, Fengxian Li, Shufang Wang, Xin Ji, Shaokun Shu, Xiangdong Fang, Lan Jiang
thousand cells or nuclei (7 µl) were added with 3 µl 25 µM RT primers (containing RT PolyT Primer (
NEB
,
S1327S
), or RT random Primer (
Thermo Scientific
,
SO142
), or their mix)), incubated for 5 min at 55 °C to resolve
(4)
Multiplex HDR for disease and correction modeling of SCID by CRISPR genome editing in human HSPCs
Molecular Therapy - Nucleic Acids
March 14, 2023
Ortal Iancu, Daniel Allen, Orli Knop, Yonathan Zehavi, Dor Breier, Adaya Arbiv, Atar Lev, Yu Nee Lee, Katia Beider, Arnon Nagler,
et al.
obtained on days 14 and 28 of IVTD. cDNA was prepared from 50 to 150 ng RNA, using Oligo d(T)23 VN-
S1327S
(
NEB
), dNTPs 10 mM, and
M-MuLV Reverse Transcriptase
(cat. no.
M0253S
,
NEB
).
Quantitative real-time PCR
(5)
Role of lncRNA LIPE-AS1 in adipogenesis
Adipocyte
December 1, 2022
Thunen A, La Placa D, Zhang Z, Shively JE.
BioLabs 5ʹ RACE protocol using a Template Switching Oligo (TSO) [86]. Briefly, an Oligo d(T) primer (
NEB
S1327S
) was annealed to the RNA template.
Template Switching RT Enzyme mix
(
NEB
M0466S
) was then used for reverse
(6)
Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene
Cells
January 15, 2022
Clees AS, Stolp V, Häupl B, Fuhrmann DC, Wempe F, Seibert M, Weber S, Banning A, Tikkanen R, Williams R,
et al
.
instructions. An amount of 3 μg of RNA was reverse-transcribed with 2 μM oligo(dT) primers (
NEB
, #
S1327S
), 2 μM random primers (New England Biolabs, Frankfurt, Germany,, #
S1330S
), dNTPs (
New England Biolabs
,
(7)
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife
July 23, 2020
Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li, Chengqi Yi
oligo,doi:10.1038/nprot.2014.006,
PCR
primers,AAGCAGTGGTATCAACGCAGAGT Sequence-based reagent,oligo dT(23)VN primer,
NEB
,Cat#:
S1327S
, Sequence-based reagent,
Random primer mix
,
NEB
,Cat#:
S1330S
, Sequence-based reagent,N501 primer,Illumina,
PCR
(8)
Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
PeerJ
November 24, 2015
Adam D. Hargreaves, John F. Mulley
reaction comprising the required amount of mRNA, 2 µl of 1 µM Oligo d(T)23 VN primer (
New England BioLabs
S1327S
; New England BioLabs, Ipswich, MA, USA), 1 µl of 10 mM dNTPs and the appropriate volume of Rnase-free
(9)
Inducible and coupled expression of the polyomavirus middle T antigen and Cre recombinase in transgenic mice: an in vivo model for synthetic viability in mammary tumour progression
Breast Cancer Research
February 1, 2014
Trisha Rao, Jill J Ranger, Harvey W Smith, Sonya H Lam, Lewis Chodosh, William J Muller
reverse transcribing the isolated RNA using
M-Mulv Reverse Transcriptase
(#
M0253S
), Oligo-dT(23VN) (#
S1327S
) and a murine RNase inhibitor (#
M0314S
) (all purchased from
New England Biolabs
). Real-time
quantitative PCR
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Oligo d(T)23VN from New England Biolabs
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