Oligo d(T)18mRNA Primer from New England Biolabs

Product Specs
ItemOligo d(T)18mRNA Primer
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS1316S
TargetOligo d(T)18 (no 5'-Phosphate)
Quantity5 a260 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
Primer TypeOligo dT Primers
TypeOligo
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolismCommunications biologyJanuary 4, 2023Landowski M, Bhute VJ, Grindel S, Haugstad Z, Gyening YK, Tytanic M, Brush RS, Moyer LJ, Nelson DW, Davis CR, et al.Scientific, Waltham, MA). One microgram of RNA was used to make cDNA using an Oligo d(T)18 primer (#S1316S, NEB, Ipswich, MA) and the ProtoScript II Reverse Transcriptase (#M0368L, NEB, Ipswich, MA) following

(2) A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecupsScientific reportsJanuary 14, 2022Landowski M, Bhute VJ, Takimoto T, Grindel S, Shahi PK, Pattnaik BR, Ikeda S, Ikeda A.Scientific, Waltham, MA). Equal RNA concentrations were aliquoted to make cDNA using an Oligo d(T)18 primer (#S1316S, NEB, Ipswich, MA) and the ProtoScript II Reverse Transcriptase (#M0368L, NEB, Ipswich, MA). Triplicate

(3) Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic MacrophagesFrontiers in immunologyJanuary 1, 2022Gargani S, Lourou N, Arapatzi C, Tzanos D, Saridaki M, Dushku E, Chatzimike M, Sidiropoulos ND, Andreadou M, Ntafis V, et al.cDNA synthesis 0.5-2μg of total RNA was reverse transcribed using MMLV-RT (Promega, M1705) and Oligo dT (NEB, S1316S) at 37°C for 1h. qRT-PCR was performed using EvaGreen SsoFast mix (Bio-Rad, Hercules, CA, USA, 172-5201)

(4) Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma TumorsCancersOctober 13, 2021Azatyan A, Zhang S, Darabi A, Siesjö P, Wang T, Zaphiropoulos PG.performed as described above. Moreover, cDNA synthesis was also performed using oligo(dT) primers (S1316S, NEB, Ipswich, MA, USA). In the reverse transcription reaction, while random hexamers prime both linear

(5) Head and neck squamous cell carcinoma cell lines have an immunomodulatory effect on macrophages independent of hypoxia and toll-like receptor 9BMC cancerSeptember 3, 2021Ishizu T, Eichin D, Padzik A, Tuominen S, Grénman R, Salmi M, Grönroos TJ, Tuomela JM.RiboLock RNase inhibitor (Thermo Scientific, #EO0381) and the oligo-dT mRNA primer (New England BioLabs, #S1316S). 100 ng of cDNA from the in vitro samples was amplified with DyNAmo HS SYBR Green (Thermo Scientific,

(6) SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by AktDevelopmental cellApril 19, 2021Burckhardt CJ, Minna JD, Danuser G.using SuperScript IV (Thermo Scientific, 18091050). In brief, 1 μg mRNA, oligo-d(T)18 primer (NEB, S1316S) and dNTPs (Bio Basic) were heated to 65 °C for 5 minutes and annealed on ice for three minutes. SuperScript

(7) Modulation of Tmem135 Leads to Retinal Pigmented Epithelium Pathologies in MiceInvestigative ophthalmology & visual scienceOctober 1, 2020Landowski M, Grindel S, Shahi PK, Johnson A, Western D, Race A, Shi F, Benson J, Gao M, Santoirre E, et al.Scientific, Waltham, MA, USA). Equal RNA concentrations were used to make cDNA using an Oligo d(T)18 primer (#S1316S; NEB, Ipswich, MA, USA) and the ProtoScript II Reverse Transcriptase (#M0368L; NEB) following the manufacturer's

(8) Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal TumorigenesisCell reportsJanuary 15, 2019Koliaraki V, Chalkidi N, Henriques A, Tzaferis C, Polykratis A, Waisman A, Muller W, Hackam DJ, Pasparakis M, Kollias G.Transcriptase,Promega,M1701 Platinum SYBR-Green qPCR SuperMix,Invitrogen,11733046 Oligo-dT primers,New England BioLabs,S1316S Azoxymethane (AOM),Sigma-Aldrich,A5486 Dextran Sodium Sulfate (DSS),MP Biomedicals,M9147 LPS from

(9) Translational Reprogramming Provides a Blueprint for Cellular AdaptationCell Chemical BiologyNovember 1, 2018Max Berman Ferretti, Jennifer Louise Barre, Katrin KarbsteinDNase,Promega,CAT# M6101 ProtoScriptII,New England Biolabs,CAT# M0368S Oligo dT,New England Biolabs,CAT# S1316S Excella™ SYBR MasterMix IQ,Worldwide Medical Products,CAT# 61071103 Experimental Models: Cell Lines,Experimental Models

(10) Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscleMuscle & NerveOctober 1, 2017Curtis A. Nutter, Elizabeth Jaworski, Sunil K. Verma, Yareli Perez‐Carrasco, Muge N. Kuyumcu‐Martinezas described elsewhere.27,34 Briefly, a cDNA library was generated by annealing 125 ng of oligo(dT) (S1316S; New England BioLabs, Ipswich, Massachusetts) to the poly(A) tail of total RNA (1 μg) at 65° C for 10

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