MspI from New England Biolabs

Product Specs
ItemMspI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0106T
Quantity5000 units
Concentration100,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.15 min at 37°C. 25 μL of CutSmart buffer (25 μL; 10x, NEB, B7204S) and 2 μL of MspI (100 U/μl; NEB, R0106M) were added. After 4 and 8 h of incubation at 37°C, additional 2 μL of MspI were added for a total of

(2) Adverse Maternal Environments Perturb Hepatic DNA Methylome and Transcriptome Prior to the Adult-Onset Non-Alcoholic Fatty Liver Disease in Mouse OffspringNutrientsApril 30, 2023Qi Fu, Warren A. Cheung, Amber V. Majnik, Xingrao Ke, Tomi Pastinen, Robert H. Laneprepared using 250 ng of genomic DNA, digested with Msp1 (New England Biolabs (NEB), Ipswich, MA, USA, R0106M), and purified using the QIAquick Nucleotide Removal Kit (Qiagen, 28004). End-repair A tailing was performed

(3) KrasG12D induces changes in chromatin territories that differentially impact early nuclear reprogramming in pancreatic cellsGenome BiologyDecember 1, 2021Angela J. Mathison et al.preparation, and sequencing. Briefly, DNA (250 ng) was digested with Msp1 (New England Biolabs (NEB), R0106M) and purified using Qiaquick Nucleotide Removal Kit (Qiagen, 28004). End-repair A tailing was performed

(4) Rapid adaptation through genomic and epigenomic responses following translocations in an endangered salmonidEvolutionary ApplicationsOctober 1, 2021Marco Crotti, Elizabeth Yohannes, Ian J. Winfield, Alex A. Lyle, Colin E. Adams, Kathryn R. Elmer

(5) Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cellsNature BiotechnologySeptember 1, 2021Sarah J. Shareef, Samantha M. Bevill, Ayush T. Raman, Martin J. Aryee, Peter van Galen, Volker Hovestadt, Bradley E. Bernsteinpurified DNA or lysis buffer containing sorted cells. The final reaction contains 10 U MspI enzyme (NEB R0106M), 800 U T4 DNA ligase (NEB M0202M), 1.5 mM ATP (NEB P0756L), 10 nM annealed barcoded adapter, and 1x

(6) Effect of concentration and duration of particulate matter exposure on the transcriptome and DNA methylome of bronchial epithelial cellsEnvironmental EpigeneticsFebruary 28, 2021Steven K Huang, Priya Tripathi, Lada A Koneva, Raymond G Cavalcante, Nathan Craig, Anne M Scruggs, Maureen A Sartor, Furong Deng, Yahong Chenrepaired, A-tailed, and ligated (all using reagents from New England Biolabs, Ipswich, MA, catalog #s: R0106M, M0203L, M0210L, M0212L) to pair-end methylated adapters from Illumina. DNA was then resolved on agarose

(7) Polyglutamine-expanded ataxin3 alter specific gene expressions through changing DNA methylation status in SCA3/MJDAging (Albany NY)February 15, 2021Dongxue Ding, Chunrong Wang, Zhao Chen, Kun Xia, Beisha Tang, Rong Qiu, Hong JiangBGI-Shenzhen (Shenzhen, China). First, 3 μg of total genomic DNA from each sample was digested with MspI (NEB, R0106T), and then, DNA end repair, single A nucleotide addition, and multiplexed adapter ligation were performed.

(8) Alteration of the brain methylation landscape following postnatal inflammatory injury in rat pupsThe FASEB JournalJanuary 1, 2020Wyston C. Pierre, Lisa‐Marie Legault, Irene Londono, Serge McGraw, Gregory A. LodygenskyBriefly, 600 ng of genomic DNA was digested over night with non‐methylation specific Msp1 enzyme (NEB # R0106M). Ligation of methylated adaptors (NEB # E7535L) was done on the digested DNA with T4 ligase enzyme

(9) A Unique Epigenomic Landscape Defines Human ErythropoiesisCell reportsSeptember 10, 2019Schulz VP, Yan H, Lezon-Geyda K, An X, Hale J, Hillyer CD, Mohandas N, Gallagher PG.04434 MethoCult H4330,StemCell Technologies,Cat# 04330 MspI restriction enzyme,New England Biolabs,Cat# R0106T T4 DNA polymerase,New England Biolabs,Cat# M0203S T4 DNA ligase reaction buffer,New England Biolabs,Cat#

(10) Rapid Multiplexed Reduced Representation Bisulfite Sequencing Library Prep (rRRBS)BIO-PROTOCOLFebruary 20, 2019Lisa-Marie Legault, Donovan Chan, Serge McGrawλ phage DNA (Promega, catalog number: D1521) 7. MspI 100 U/μl (New England Biolabs, catalog number: R0106M) 8. Klenow fragment 5,000 U/ml (New England Biolabs, catalog number: M0212L) 9. dNTP set (4 x 100 mM)

(11) Identifying DNA methylation biomarkers for non-endoscopic detection of Barrett’s esophagusScience Translational MedicineJanuary 17, 2018Helen R. Moinova et al.isolated from frozen esophageal biopsies or cell lines was digested with MspI (New England Biolabs # R0106T). End repair, A-tailing, and adapter ligation were carried out using the NEXTFlex Bisulfite-sequencing

(12) Identification of differential genomic DNA Methylation in the hypothalamus of pubertal rat using reduced representation Bisulfite sequencingReproductive Biology and EndocrinologyDecember 1, 2017Lei Luo et al.(Shenzhen, China). Briefly, at least 3 μg of total genomic DNA from each sample was digested with MspI (NEB, R0106T). The digestion was followed by DNA end-repair, the addition of a single A nucleotide, and multiplexed

(13) Methylation-assisted bisulfite sequencing to simultaneously map 5fC and 5caC on a genome-wide scale for DNA demethylation analysisNature ProtocolsJuly 1, 2016Francesco Neri, Danny Incarnato, Anna Krepelova, Caterina Parlato, Salvatore Oliviero600410) • dNTP Set 100mM (Life Technologies, cat. no. 10297) • MspI (New England Biolabs, cat. no. R0106M) • M.SssI CpG Methyltransferase (New England Biolabs, cat. no. M0226M) • HpaII (New England Biolabs,

(14) Identification of differentially methylated regions in new genes associated with knee osteoarthritisGeneJanuary 15, 2016Carolina A. Bonin, Eric A. Lewallen, Saurabh Baheti, Elizabeth W. Bradley, Michael J. Stuart, Daniel J. Berry, Andre J. van Wijnen, Jennifer J. Westendorfthe DNeasy Kit (Qiagen). DNA (250ng) was digested with Msp1 (New England Biolabs, Catalog0 Number: R0106M) and purified using Qiaquick Nucleotide Removal Kit (Qiagen, Catalog Number: 28004). End-repair A tailing

(15) Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defectsEpigenetics & ChromatinDecember 1, 2015Kylee J. Veazey, Scott E. Parnell, Rajesh C. Miranda, Michael C. GoldingM0357S; NEB) at 37 °C overnight. Glycosylated genomic DNA was then digested with 100 units of MspI (Cat# R0106M; NEB) or 50 units of HpaII (Cat# R0171L; NEB), or no enzyme at 37 °C overnight. Reactions were inactivated

(16) Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenaseseLifeJuly 31, 2015Brad Evans, Erin Griner, Reproducibility Project: Cancer BiologyElmer,BLU502H/NEG502H,, MspI methyltransferase,Reagent,NEB,M0215L,, MspI restriction endonuclease,Reagent,NEB,R0106T,, DNA duplex oligonucleotide substrate,oligo,Integrated DNA Technologies,"custom 5′-GTGTTCTTTCAGCTCCGGTCACGCTGACCAGC-3′

(17) Nanopores Suggest a Negligible Influence of CpG Methylation on Nucleosome Packaging and StabilityNano LettersJanuary 14, 2015Martin Langecker, Andrey Ivankin, Spencer Carson, Shannon R. M. Kinney, Friedrich C. Simmel, Meni Wanunuconcentration (10 μM). Methylation of DNA was determined through restriction digestion with the MspI (NEB R0106M) and HpaII (NEB R0171M) isoschizomer restriction enzymes as per the provided protocols. Both of these

(18) A genome-wide 3C-method for characterizing the three-dimensional architectures of genomesMethodsNovember 1, 2012Zhijun Duan, Mirela Andronescu, Kevin Schutz, Choli Lee, Jay Shendure, Stanley Fields, William S. Noble, C. Anthony Anthony Blau21 UltraPure™ Phenol:Chloroform:Isoamyl Alcohol,Invitrogen,15593-031,Step 22 MseI,NEB,R0525M,Step 25 MspI,NEB,R0106M,Step 25 Qiaquick PCR purification kit,Qiagene,28106,"Steps 26, 30,32, 33, 35, 36, 38, 40" Plasmid-Safe™

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MspI

MspI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (18)

Reviews

MspI from New England Biolabs

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