DNA Polymerase I, Large (Klenow) Fragment from New England Biolabs

Product Specs
ItemDNA Polymerase I, Large (Klenow) Fragment
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0210S
Quantity200 units
Concentration5 U/µl
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmoles of dNTPs to an acid-insoluble form in 30 minutes at 37°C.

Comments:
DNA sequencing by the Sanger dideoxy method Fill-in of 3´ recessed ends Second-strand cDNA synthesis Random priming labeling. Second strand synthesis in mutagenesis protocols.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Outward-oriented sites within clustered CTCF boundaries are key for intra-TAD chromatin interactions and gene regulationNat CommunDecember 7, 2023Xiao Ge, Haiyan Huang, Keqi Han, Wangjie Xu, Zhaoxia Wang, Qiang Wubiotin-14-dATP (Thermo, 19524016), dCTP, dTTP, and dGTP using the Klenow fragment of DNA polymerase I (NEB, M0210L), and ligated using T4 DNA ligase (NEB, M0202S). After ligation, the nuclei were de-cross-linked overnight

(2) Cellular reprogramming is driven by widespread rewiring of promoter-enhancer interactionsBMC BiologyNovember 20, 2023Miao Wang, Bing He, Yueling Hao, Divyaa Srinivasan, Jatin Shrinet, Peter FrasermM dGTP, 1.56 μL of 10 mM dTTP, and 10.2 μL of 5U/μL DNA polymerase I, Large (Klenow) Fragment (NEB, M0210L) and incubated the reaction at 37 °C for 1 h. In-nucleus ligation mix was prepared by adding 102μL 10X

(3) Genome folding principles revealed in condensin-depleted mitotic chromosomesbioRxivNovember 17, 2023Han Zhao, Yinzhi Lin, En Lin, Fuhai Liu, Lirong Shu, Dannan Jing, Baiyue Wang, Manzhu Wang, Fengnian Shan, Lin Zhang, et al.Biotin-14-dATP (active motif, Catalog No: 14138) using 6.6U DNA Polymerase I, Large (Klenow) fragment (NEB, Cat#M0210). Chromatin was then ligated in-situ with 2000U T4 DNA ligase (NEB, Cat#M0202M) for 4 hours at 16°C

(4) Lineage specific 3D genome structure in the adult human brain and neurodevelopmental changes in the chromatin interactomeNucleic Acids ResearchNovember 10, 2023Samir Rahman, Pengfei Dong, Pasha Apontes, Michael B Fernando, Roman Kosoy, Kayla G Townsley, Kiran Girdhar, Jaroslav Bendl, Zhiping Shao, Ruth Misir, et al.dTTP (Sigma-Aldrich, Cat# DNTP10-1KT), 10 μl of 5 U/μl Klenow DNA Pol I (New England Biolabs, Cat# M0210L), 22.5 μl of 1× CutSmart buffer) and shaking at 37°C for 1.5 h at 400 rpm. Blunt ended sites were proximity

(5) Context-dependent neocentromere activity in synthetic yeast chromosome VIIICell GenomicsNovember 8, 2023Stephanie Lauer, Jingchuan Luo, Luciana Lazar-Stefanita, Weimin Zhang, Laura H. McCulloch, Viola Fanfani, Evgenii Lobzaev, Max A.B. Haase, Nicole Easo, Yu Zhao, et al.water. DNA sticky ends were filled with biotin-14-dCTP (Invitrogen, 19518018) by Klenow enzyme (NEB, M0210L) at 37°C for 80 min. Biotinylated DNA was ligated with 60 U T4 DNA ligase (Thermo Scientific, EL0014)

(6) Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and functionCell GenomicsNovember 8, 2023Jingchuan Luo, Luis A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang, et al."T4 Polynucleotide Kinase (10,000 units/ml)",New England BioLabs,M0201S Klenow Fragment,New England BioLabs,M0210S T4 DNA Polymerase,New England BioLabs,M0203S dATP (100 mM),ThermoFisher Scientific,10216018 Klenow

(7) The landscape of the histone-organized chromatin of Bdellovibrionota bacteriabioRxivNovember 7, 2023Georgi K. Marinov, Benjamin Doughty, Anshul Kundaje, William J. Greenleafeach of 10 mM dCTP, dGTP and dTTP, and 8 μL of 5U/μL DNA Polymerase I Large (Klenow) Fragment (NEB #M0210). The reaction was the incubated at 37 °C in a Thermomixer at 900 rpm for 45 minutes. Fragment end ligation

(8) MGA-seq: robust identification of extrachromosomal DNA and genetic variants using multiple genetic abnormality sequencingGenome BiologyOctober 30, 2023Da Lin, Yanyan Zou, Xinyu Li, Jinyue Wang, Qin Xiao, Xiaochen Gao, Fei Lin, Ningyuan Zhang, Ming Jiao, Yu Guo, et al.r.p.m. End filling-in Add 5 μl of dNTP mix (10 mM each) and 5 μl of DNA polymerase I Klenow fragment (NEB, M0210) to the reaction system, place the sample in thermomixer with rotation at 37 °C at 1000 r.p.m for 30

(9) Pioneer activity distinguishes activating from non‐activating SOX2 binding sitesThe EMBO JournalOctober 16, 2023Michela Maresca, Teun van den Brand, Hangpeng Li, Hans Teunissen, James Davies, Elzo de WitPolynucleotide kinase PNK 200 U/ml (NEB M0201L); DNA Polymerase I Large (Klenow) Fragment 100 U/ml (NEB M0210L) and T4 DNA ligase 300 U/ml (Thermo Scientific EL0013). The reaction was incubated at 37°C for 2 h followed

(10) On the rules of engagement for microRNAs targeting protein coding regionsNucleic Acids ResearchOctober 13, 2023Sunil Sapkota, Katherine A Pillman, B Kate Dredge, Dawei Liu, Julie M Bracken, Saba Ataei Kachooei, Bradley Chereda, Philip A Gregory, Cameron P Bracken, Gregory J GoodallDNA sequencing of the mCherry reporter with the same radiolabelled primers and Klenow DNA polymerase (NEB M0210) was used as a ladder to map the cleavage sites to nucleotide resolution. Products were separated by

(11) Regulation of CTCF loop formation during pancreatic cell differentiationNature CommunicationsOctober 9, 2023Xiaowen Lyu, M. Jordan Rowley, Michael J. Kulik, Stephen Dalton, Victor G. CorcesNaCl, 10 mM Tris, 10 mM MgCl2, 100 μg/ml BSA, 2 mM ATP, 3 mM DTT, 8 μl Large Klenow Fragment (NEB, #M0210L) and 2 μl T4 PNK (NEB, #M0201L) at 37 °C for 15 min. The DNA overhangs were filled with biotinylated

(12) Identification of Multiple Novel Viruses in Fecal Samples of Black-Necked Cranes Using Viral Metagenomic MethodsVirusesOctober 9, 2023Qifan Zhao, Ran Zhao, Yijie Sun, Li Ji, Yuan Xi, Xiaochun Wang, Quan Shen, Likai Ji, Yan Wang, Zhenqiang You, et al.random primers. The complementary chain of cDNA was generated using Klenow Fragment DNA polymerase (M0210L, New England Biolabs, Rowley, MA, USA). Next, libraries were constructed using the Nextera XT DNA Sample

(13) Optimizing the Conditions for Whole-Genome Sequencing of Avian ReovirusesVirusesSeptember 16, 2023Sonsiray Alvarez Narvaez, Telvin L. Harrell, Olatunde Oluwayinka, Holly S. Sellers, Zubair Khalid, Ruediger Hauck, Erfan U. Chowdhury, Steven J. Conradstrand synthesis was performed immediately using Klenow polymerase (New England Biolabs, product number M0210S). First, 1 μL of primer 10 μM R8N (Integrated DNA Technologies (IDT)), 1 µL of 10 mM dNTP mix (New England

(14) AP profiling resolves co-translational folding pathway and chaperone interactions in vivobioRxivSeptember 8, 2023Xiuqi Chen, Christian M. Kaiserfrom miniprep without introducing non-specific cleavages in the dsDNA region. Klenow fragment (NEB, M0210S) was added to the purified DNA for end-polishing at the final concentration of 1 U per microgram of

(15) Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phaseThe EMBO journalAugust 15, 2023Batty P, Langer CC, Takács Z, Tang W, Blaukopf C, Peters JM, Gerlich DW.[Thermo Fisher Scientific, R0162], dTTP [Thermo Fisher Scientific, R0172], 50 U Klenow Polymerase [NEB, M0210L], 1× NEB 2 buffer, B7002S). Cells were then incubated for 1 h at 37°C with gentle rotation. Cells were

(16) lncRNA read-through regulates the BX-C insulator Fub-1eLifeAugust 10, 2023Ibragimov A, Bing XY, Shidlovskii YV, Levine M, Georgiev P, Schedl P.units,NEB,M0201S, "Chemical compound, drug","DNA Polymerase I, Large (Klenow) Fragment – 1000 units",NEB,M0210L, "Chemical compound, drug",End-it DNA End Repair Kit,Thermo Fisher Scientific,NC0105678, "Chemical

(17) Teosinte Pollen Drive guides maize domestication and evolution by RNAibioRxivAugust 3, 2023Benjamin Berube, Evan Ernst, Jonathan Cahn, Benjamin Roche, Cristiane de Santis Alves, Jason Lynn, Armin Scheben, Adam Siepel, Jeffrey Ross-Ibarra, Jerry Kermicle, et al.eluted in 50ul. Second-strand synthesis was performed using 5U Klenow fragment (New England Biolabs, cat#M0210S) with 100uM dNTPs and 1uM of iPARE adapter primer (5’-NNNNTCTAGAATGCATGGGCCCTCCAAG-3’) for 1 hour at

(18) RNA polymerase II dynamics shape enhancer–promoter interactionsNature GeneticsAugust 1, 2023Gilad Barshad, James J. Lewis, Alexandra G. Chivu, Abderhman Abuhashem, Nils Krietenstein, Edward J. Rice, Yitian Ma, Zhong Wang, Oliver J. Rando, Anna-Katerina Hadjantonakis, et al.rSAP (NEB #M0371) and end polishing using T4 PNK (NEB #M0201), DNA polymerase Klenow fragment (NEB #M0210) and biotinylated dATP and dCTP (Jena Bioscience #NU-835-BIO14-S and #NU-809-BIOX-S, respectively),

(19) Aub, Vasa and Armi localization to phase separated nuage is dispensable for piRNA biogenesis and transposon silencing in DrosophilabioRxivJuly 29, 2023Samantha Ho, Nicholas P Rice, Tianxiong Yu, Zhiping Weng, William E TheurkaufN0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3ʹ to 5ʹ exo,NEB,Cat# M0212L UDG,NEB,Cat# M0280S Phusion Polymerase,NEB,Cat#

(20) E74‑like ETS transcription factor 5 facilitates cell proliferation through regulating the expression of adenomatous polyposis coli 2 in non‑small cell lung cancerInternational Journal of Molecular MedicineJuly 11, 2023Jing Wen, Genggeng Qin, Zhaojing Jiang, Zixun Lin, Ruixin Zhou, Hui Dai, Zhanfa Xu, Weiguo Chen, Qiancheng SongSecond strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP (cat. nos. M0210L and N0447L, respectively; New England BioLabs, Inc.). PCR amplification was then performed. The PCR product

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