MACSmix Tube Rotator from Miltenyi Biotec

Product Specs
ItemMACSmix Tube Rotator
CompanyMiltenyi Biotec
Price Pricing Info Get Pricing Get Quote for this Product
Catalog Number130-090-753
ApplicationsSample preparation
QuantityEA
Regulatory StatusFor research use only
ComponentsMACSmix Tube Rotator : Power supply : 4x Rechargeable NiMH batteries, type AA : Screwdriver : Large rack for tubes from 5 mL to 50 mL in size : Small rack for tubes from 0.5 mL to 2 mL in size : User manual
Capacity16 tubes with 0.5 mL, 1.5 mL, or 2 mL
4 × 15 mL tubes
4 × 50 mL tubes, or
4 × gentleMACS Tubes
Dimensions(W x D x H) 281 mm × 96 mm x 110 mm)
Speed RangeContinuous mixing: 12 rpm, 16 rpm, or 20 rpm
Interval mixing: 30 seconds at 12 rpm, 1 minute or 5 minutes pause
Temperature Range+2 °C to +42 °C
Electrical RequirementsInput voltage 100–240 V~
Frequency 50/60 Hz

Add to Compare List

Local Office

Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) Human in vitro-induced IL-17A+ CD8+ T-cells exert pro-inflammatory effects on synovial fibroblastsClinical and Experimental ImmunologyDecember 11, 2023Elizabeth H Gray, Ushani Srenathan, Lucy E Durham, Sylvine Lalnunhlimi, Kathryn J A Steel, Anca Catrina, Bruce W Kirkham, Leonie S Taamssuspension was diluted with warm complete culture medium and incubated in a continuous motion using the MACSmix rotator (Miltenyi Biotec) for 45 minutes at 37°C (5% CO2) to allow secretion and capture of IL-17A on

(2) Cell surface profiling of cultured cells by direct hydrazide capture of oxidized glycoproteinsMethodsXDecember 1, 2023Tammy-Lynn Tremblay, François Fauteux, Deborah Callaghan, Jennifer J. HillBuffer (see below).-15 mL plastic conical tubes-1 mL pipette and tips-3 mL syringe with a 33 gage needle-MACSMix head-over-head shaker (Miltenyi Biotec) or a similar tube rotator-Swinging-bucket centrifuge capable

(3) Single cell dynamics of tumor specificity vs bystander activity in CD8+ T cells define the diverse immune landscapes in colorectal cancerCell DiscoveryNovember 15, 2023Daniel Morales Borràs, Sara Verbandt, Markus Ausserhofer, Gregor Sturm, Jinyeong Lim, Gil Arasa Verge, Isaure Vanmeerbeek, Raquel S. Laureano, Jannes Govaerts, Jenny Sprooten, et al.Memorial Institute (RPMI) 1640 medium). Gentle MACS programs (h_tumor_01, 02 and 03) were run in a MACSmix Tube Rotator (Miltenyi) with two 30-min incubation periods at 37 °C between each run. The digested samples were filtered

(4) Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney diseaseNature CommunicationsNovember 15, 2023Ann-Christin Gnirck, Marie-Sophie Philipp, Alex Waterhölter, Malte Wunderlich, Nikhat Shaikh, Virginia Adamiak, Lena Henneken, Tobias Kautz, Tingting Xiong, Daniela Klaus, et al.mg/ml, Roche, Basel, Switzerland) and DNase I (100 µg/ml, Roche) for 45 min at 37 °C while rotating on a MACSmix® tube rotator (Miltenyi, Bergisch Gladbach, Germany). After further dispersion with the gentleMACS® dissociator (Miltenyi),

(5) Single‐cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomaEMBO Molecular MedicineNovember 8, 2023Lise Finotto, Basiel Cole, Wolfgang Giese, Elisabeth Baumann, Annelies Claeys, Maxime Vanmechelen, Brecht Decraene, Marleen Derweduwe, Nikolina Dubroja Lakic, Gautam Shankar, et al.and enzymatically dissociated with the MACS Brain Tumor Dissociation Kit, gentleMACS Dissociator and MACSmix Tube Rotator (Miltenyi Biotec) according to the manufacturer's protocol. The dissociated sample was passed through

(6) Post-ischemic inactivation of HIF prolyl hydroxylases in endothelium promotes maladaptive kidney repair by inducing glycolysisbioRxivOctober 10, 2023Ratnakar Tiwari, Rajni Sharma, Ganeshkumar Rajendran, Gabriella S. Borkowski, Si Young An, Michael Schonfeld, James O’Sullivan, Matthew J. Schipma, Yalu Zhou, Guillaume Courbon, et al.Multi_E_01. Dissociated kidneys were incubated for 30 minutes at 37 °C with continuous rotation using the MACSmix Tube Rotator (Miltenyi Biotec). GentleMACS C tubes were then placed in the gentleMACS Dissociator and Multi_E_02

(7) Single-Cell Profiling of Cells in the Lung of a Patient with Chronic Hypersensitivity Pneumonitis Reveals Inflammatory Niche with Abundant CD39+ T Cells with Functional ATPase Phenotype: A Case StudyInternational Journal of Molecular SciencesSeptember 22, 2023Tharushi Ayanthika de Silva, Simon Apte, Joanne Voisey, Kirsten Spann, Maxine Tan, Chandima Divithotawela, Daniel Chambers, Brendan O’Sullivanexplant lungs, minced using scissors, and then incubated with digestion media for one hour at 37 °C on a MACSmix™ tube rotator (Miltenyi Biotec, Bergisch Gladbach, Germany) at maximum speed. Disaggregated cells were sieved using

(8) Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse ModelInternational Journal of Molecular SciencesSeptember 10, 2023Eun-Ji Lee, Jang-Gi Choi, Jung Ho Han, Yong-Wan Kim, Junmo Lim, Hwan-Suck Chungwith 1 mg/mL collagenase, 1 mg/mL trypsin inhibitor, and 0.1 mg/mL DNase for 40 min at 37°C using a MACSmix Tube Rotator (Miltenyi Biotec) for enzymatic digestion with a Tumor Dissociation Kit (Miltenyi Biotec). The tumor

(9) Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasisNature communicationsAugust 23, 2023Zhang S, Fang W, Zhou S, Zhu D, Chen R, Gao X, Li Z, Fu Y, Zhang Y, Yang F, et al.segments and underwent cell lysis using gentleMACS (130-093-235; Miltenyi Biotec Germany) and MACSmix (130-090-753; Miltenyi Biotec Germany) in the mixed enzyme solution (4.7 mL Medium, 200 µL Enzyme H, 100 µL Enzyme

(10) Dynamic thresholding and tissue dissociation optimization for CITE-seq identifies differential surface protein abundance in metastatic melanomaCommun BiolAugust 10, 2023Ulrike Lischetti, Aizhan Tastanova, Franziska Singer, Linda Grob, Matteo Carrara, Phil F. Cheng, Julia M. Martínez Gómez, Federica Sella, Veronika Haunerdinger, Christian Beisel, et al.30 min to 3 h at 37 °C with continuous rotation on a MACSmix tube rotator (Miltenyi Biotec, cat. no. 130-090-753) and trituration every 15 min using wide-bore pipet tips (Supplementary Note 1). After incubation with

(11) Two different isoforms of osteopontin modulate myelination and axonal integrityFASEB bioAdvancesAugust 1, 2023Nilsson G, Mottahedin A, Zelco A, Lauschke VM, Ek CJ, Song J, Ardalan M, Hua S, Zhang X, Mallard C, et al.the Neural Tissue Dissociation kit (NTDK‐P, 130‐0292‐628, Miltenyi Biotec), a MACSmix tube rotator (130‐090‐753, Miltenyi Biotec), and a gentle MACS Dissociator (130‐093‐235, Miltenyi Biotec) according to the manufacturer's

(12) Clonally expanded CD38 hi cytotoxic CD8 T cells define the T cell infiltrate in checkpoint inhibitor–associated arthritisScience ImmunologyJuly 28, 2023Runci Wang, Anvita Singaraju, Kathryne E. Marks, Lorien Shakib, Garrett Dunlap, Ifeoluwakiisi Adejoorin, Stinne R. Greisen, Lin Chen, Aidan K. Tirpack, Carlos Aude, et al.medium + Liberase TL (100μg/ml; Roche) + DNaseI (100μg/ml; Roche). The tubes were placed securely in a MACSmix tube rotator (Miltenyi Biotec) and placed in an incubator at 37°C, 5% CO2 for 30 minutes. The digested tissue was

(13) Identification of a novel cord blood NK cell subpopulation expressing functional programmed death receptor-1Frontiers in ImmunologyJune 22, 2023Marco Greppi, Valentina Obino, Rayan Goda, Federico Rebaudi, Simona Carlomagno, Mariella Della Chiesa, Simona Sivori, Gianluca Ubezio, Vanessa Agostini, Alessandra Bo, et al.indicated in the appropriate Figure, for 3 h at 37°C and 5% CO2 under slow continuous rotation using the MACSmix Tube Rotator (Miltenyi Biotec). After capturing secreted cytokines at their surface, cells were centrifuged at 300

(14) Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysisCommunications BiologyMay 16, 2023William Chour, Jongchan Choi, Jingyi Xie, Mary E. Chaffee, Thomas M. Schmitt, Kathryn Finton, Diana C. DeLucia, Alexander M. Xu, Yapeng Su, Daniel G. Chen, et al.DCs were loaded with 1 µg/mL of pooled HLA-restricted peptides and incubated for 4 h at 37 °C in a MACSmix™ Tube Rotator (Miltenyi Biotec). CD8+ T cells were isolated from autologous PBMCs using the EasySep™ Human CD8+ T Cell Enrichment Kit

(15) Engineered combinatorial cell device for wound healing and bone regenerationFrontiers in Bioengineering and BiotechnologyMay 10, 2023Lucija Kadunc Polajnar, Duško Lainšček, Rok Gašperšič, Petra Sušjan-Leite, Uroš Kovačič, Miha Butinar, Boris Turk, Roman Jerala, Iva Hafner-Bratkovičadded, and the tissue was incubated for an hour at 37°C, in 5% CO2, under continuous rotation using the MACSmix Tube Rotator (Miltenyi Biotec). Following incubation, normal culture medium (DMEM supplemented with 10% FBS) was

(16) Effective Endotoxin Removal from Chitosan That Preserves Chemical Structure and Improves Compatibility with Immune CellsPolymers (Basel)March 23, 2023Sophie L. Reay, Emma L. Jackson, Daniel Salthouse, Ana Marina Ferreira, Catharien M. U. Hilkens, Katarina NovakovicTLR4 inhibitor CLI-095 (5 µg/mL) in 15 mL falcon tubes and incubated at 37 °C with 5% CO2 for 6 h on a MACSmix™ Tube Rotator (Miltenyi Biotec, Bergisch Gladbach, Germany). PBMCs were then added to an uncoated or chitosan-coated

(17) Integrated transcriptome study of the tumor microenvironment for treatment response prediction in male predominant hypopharyngeal carcinomaNature CommunicationsMarch 16, 2023Yang Zhang, Gan Liu, Minzhen Tao, Hui Ning, Wei Guo, Gaofei Yin, Wen Gao, Lifei Feng, Jin Gu, Zhen Xie, et al.transferred into gentleMACS C tube (Miltenyi Biotec), and ran h_TDK_3 program according to User Manual of MACSmix Tube Rotator (Miltenyi Biotec) and then filtered twice using a 40-µm nylon mesh (Thermo Fisher Scientific). After

(18) A protocol to detect human CD4+ T cell extracellular traps using scanning electron microscopySTAR ProtocolsMarch 1, 2023Nam Phuong N. Nguyen, Nicole Chizara Oparaugo, Kelsey Ouyang, George W. AgakInc,16007 Double sided carbon tape,Electron Microscopy Sciences,77817 MACSmix Tube rotator,Miltenyi Biotec,130-090-753 FACS Vantage,BD Biosciences, Nunc Microwell Terasaki Plates,Thermo Scientific,438733 Mitsubishi AnaeroPack-Anaero,Thermo

(19) Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysatesSTAR ProtocolsMarch 1, 2023Robert J. Smith, Rachel Milne, Victoriano Corpas Lopez, Natalie Wiedemar, Gourav Dey, Aisha J. Syed, Stephen Patterson, Susan Wyllieal.8,https://maxquant.net/perseus/ ,, Other,Other,Other ,, NuPAGE 4–12% Bis Tris Gel,Invitrogen,NP0321 Macsmix™ tube rotator,Miltenyi Biotec,130-090-753 Apex 1.5 mL screw-cap microcentrifuge tube,Alpha Laboratories,CP5911 Apex 1.5 mL screw-cap microcentrifuge

(20) Hypoxia-Driven TGFβ Modulation of Side Population Cells in Breast Cancer: The Potential Role of ERαCancersFebruary 9, 2023Mallini P, Chen M, Mahkamova K, Lennard TWJ, Pan Y, Wei D, Stemke-Hale K, Kirby JA, Lash GE, Meeson A.was added to one tube containing 1 × 106 cells/mL. All cell samples were incubated at 37 °C using a MACsMix rotor (Miltenyi Biotec, Surrey, UK) for 15 min. All cell samples were then treated with Hoechst 33342

Write a Review

(1)

4/15/2022Great Tube Rotator Rebecca GuyerWe have many uses for rotating tubes of various sides and this rotator works well and is small enough to fit anywhere, including the cold room.
(1)

3/21/2019Battery Operated Tube Rotator Richard CattleyProduct works great and we love the fact it can run on battery power. This makes moving it between incubators, benchtop, and cold rooms super easy.
(1)

4/25/2016Really Practical in the Lab Jesus CirizaIt is autonomous and it does not need to be connected to electricity. It can be used without connecting to a electrical plug for a long period o time