HhaI from New England Biolabs

Product Specs
ItemHhaI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0139L
Quantity10000 units
Concentration20 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCG/C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Correlation of blood–brain barrier leakage with cerebral small vessel disease including cerebral microbleeds in Alzheimer's diseaseFrontiers in NeurologyFebruary 16, 2023Zhaozhao Cheng, Linbin Dai, Yan Wu, Yuqin Cao, Xianliang Chai, Peng Wang, Chang Liu, Ming Ni, Feng Gao, Qiong Wang, et al.DNA fragment using the primers F-5′GGCACGGCTGTCCAAGGA and R- 5′CTCGCGGATGGCGCTGAG. Next, HhaI (NEB, R0139S) was added to the mixture of the PCR products for restriction enzyme digestion. Finally, the reaction

(2) Molecular diagnosis of pancreatobiliary tract cancer by detecting mutations and methylation changes in bile sampleseClinicalMedicineJanuary 1, 2023Shun He, Fanxin Zeng, Huihui Yin, Pei Wang, Yinlei Bai, Qianqian Song, Jiangtao Chu, Zhen Huang, Yumeng Liu, Hong Liu, et al.technology.21 Briefly, DNA fragments were digested with the methylation-sensitive restriction enzyme HhaI (R0139S, New England Biolabs, MA, USA), which cleaves unmethylated, but not methylated DNA at the 4 bp restriction

(3) Characterization of Cme and Yme thermostable Cas12a orthologsCommunications BiologyDecember 1, 2022Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robbdigesting with BstXI (NEB #R0113) for 30 min at 37 °C. Substrates were also digested with HhaI (NEB #R0139), HinP1I (NEB #R0124), or FspI (NEB #R0135) to assess the ability of the method to identify 3′ overhang,

(4) Mitosis of hepatitis B virus-infected cells in vitro results in uninfected daughter cellsJHEP ReportsSeptember 1, 2022Thomas Tu, Benno Zehnder, Jochen M. Wettengel, Henrik Zhang, Sally Coulter, Vikki Ho, Mark W. Douglas, Ulrike Protzer, Jacob George, Stephan UrbanCutSmart buffer (B7204, New England Biolabs [NEB], Ipswich, MA, USA), 7.5 U RecJf (M0264, NEB), and 10 U HhaI (R0139, NEB). The combined restriction digestion and exonuclease reaction was incubated for 4 rounds of a 15-min

(5) Reversible epigenetic alterations regulate class I HLA loss in prostate cancerCommunications BiologySeptember 1, 2022Tamara S. Rodems et al.DNA was digested with AluI and HhaI restriction enzymes (New England Biolabs, Cat# R0137L and Cat# R0139L) and enriched with MBD2-MBD protein (Takara Bio, Cat# 631962). Enriched DNA was subjected to qPCR with

(6) YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesisNature geneticsAugust 1, 2022Wu BK, Mei SC, Chen EH, Zheng Y, Pan D.was confirmed by digestion with methylation sensitive restriction enzymes Hha I (New England Biolabs, R0139S) and Hpa II (New England Biolabs, R0171S) and methylation insensitive restriction enzymes Msp I (New England Biolabs

(7) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature Cell BiologyJuly 4, 2022Yingying Meng et al.We incubated 1.5 pmoles of probe DNA (CC, CL or LL) with 2 U (or 4 U when indicated) of HhaI (NEB, R0139S) restriction enzyme at 37 °C, Bsiwl (NEB, R0553S) at 55 °C or with TaiI (Thermo, ER1141) at 65 °C for

(8) Hypermethylated PCDHGB7 as a Biomarker for Early Detection of Endometrial Cancer in Endometrial Brush Samples and Cervical ScrapingsFrontiers in Molecular BiosciencesJanuary 4, 2022Jiangjing Yuan et al.For each digestion reaction, 100 ng gDNA was taken as input, and every two units of the HhaI (NEB, R0139) and HpaII (NEB, R0171) were added, making the final volume 25 μL, followed by digestion at 37°C for

(9) A sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA While Preserving Cellular DNABIO-PROTOCOLApril 20, 2021Benno Zehnder, Stephan Urban, Thomas Tu3125000036) 20. 10× Cutsmart buffer (NEB, catalog number: B7204, store at -20 °C) 21. HhaI (NEB, catalog number: R0139, store at -20 °C) 22. PCR-grade H2O (e.g., B. Braun Melsungen, for injection purposes) 23. RecJf (NEB,

(10) Common germline variants of the human APOE gene modulate melanoma progression and survivalNature medicineJuly 1, 2020Ostendorf BN, Bilanovic J, Adaku N, Tafreshian KN, Tavora B, Vaughan RD, Tavazoie SF.genotyping38. In brief, a 244 bp fragment of APOE was amplified using standard PCR and digested with HhaI (R0139S, New England Biolabs), and allele-specific products were resolved on a 15% polyacrylamide gel. The following

(11) Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A ChromatinDevelopmental cellFebruary 10, 2020Palladino J, Chavan A, Sposato A, Mason TD, Mellone BG.Recombinant Proteins" KpnI,NEB,Cat#R0142S BglII,NEB,Cat#R0144S XhoI,NEB,Cat#R0146S HhaI,NEB,Cat#R0139S HpaII,NEB,Cat#R0171S Taq DNA polymerase,NEB,Cat#M0273L T4 Ligase,NEB,Cat#M0202S Dispase/Collagenase,Roche,Cat#10269638001 SlowFade

(12) Family history of Alzheimer’s disease alters cognition and is modified by medical and genetic factorseLifeJune 18, 2019Joshua S Talboom et al.drug FailSafe PCR Enzyme and 2X PreMix Buffers Lucigen FSP995J Chemical compound, drug HHA1 NEB R0139S Other Whatman 903 Protein Saver Card VWR 05-715-121 Chemical compound, drug Ultrapure Agarose Thermo 16500500 4% Chemical compound,

(13) Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonizationPLoS pathogensMarch 1, 2019Murdoch CC, Espenschied ST, Matty MA, Mueller O, Tobin DM, Rawls JF.using primers P3 and P4 (S1 Table), followed by purification and Hha1 digest (New England Biolabs, R0139S). All mutant alleles (rdu60, rdu61, and rdu62) result in the loss of a single Hha1 restriction site

(14) Mixing alters the lytic activity of viruses in the dark oceanEcologyMarch 1, 2018Christian Winter, Nicole Köstner, Carl-Philip Kruspe, Damaris Urban, Simone Muck, Thomas Reinthaler, Gerhard J. Herndlmmol/L Mg‐acetate, 1 mg/mL BSA, pH 7.9) and 0.5 μL of restriction enzyme HhaI (20,000 units/mL; Cat. No. R0139S, both from New England BioLabs, Ipswich, Massachusetts, USA). The amount of PCR products added to the

(15) CD38 is methylated in prostate cancer and regulates extracellular NAD⁺Cancer & metabolismJanuary 1, 2018Mottahedeh J, Haffner MC, Grogan TR, Hashimoto T, Crowell PD, Beltran H, Sboner A, Bareja R, Esopi D, Isaacs WB, et al.

(16) N6-methyladenine is an epigenetic marker of mammalian early life stressScientific ReportsDecember 22, 2017Stacey L. Kigar, Liza Chang, Candace R. Guerrero, Jacqueline R. Sehring, Amelia Cuarenta, Laurie L. Parker, Vaishali P. Bakshi, Anthony P. Augercombination of HpaII (New England Biolabs, Ipswich, MA; catalog #R0171), HhaI (New England Biolabs; catalog #R0139), or Hpy188III (New England Biolabs; catalog #R0622) cut sites were inside or outside of the primer

(17) The efficacy of Cognitive training in patients with VAsCular Cognitive Impairment, No dEmentia (the Cog-VACCINE study): study protocol for a randomized controlled trialTrialsDecember 1, 2016Yi Tang, Zude Zhu, Qing Liu, Fang Li, Jianwei Yang, Fangyu Li, Yi Xing, Jianping Jiaannealing at 60 °C for 30 s, and extending at 72 °C for 30 s. The PCR products will be digested with HhaI (R0139S, New England BioLabs, Beverly, MA, USA). The fragments will be separated by electrophoresis on a 20

(18) Telomere Restriction Fragment (TRF) AnalysisBio ProtocNovember 20, 2015Ilgen Mender, Jerry W. Shaycatalog number: 19131 or 19133) Enzymes HhaI 20,000 units/ml (New England BioLabs, catalog number: R0139L) HinF1 10,000 units/ml (New England BioLabs, catalog number: R0155L) MspI 20,000 units/ml (New England BioLabs

(19) Gadd45b is an epigenetic regulator of juvenile social behavior and alters local pro-inflammatory cytokine production in the rodent amygdalaBrain, Behavior, and ImmunityMay 1, 2015Stacey L. Kigar, Liza Chang, Anthony P. Augerdigested with either HpaII (New England Biolabs; catalog #R0171) or HhaI (New England Biolabs; catalog #R0139) in the same buffer conditions at 37 °C for 90 min. Enzymes were subsequently inactivated by heating

(20) Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometryBMC MicrobiologyMarch 7, 2014Marc Rüger, Mandy Ackermann, Udo ReichlExtraction Kit, QIAGEN, Hilden, Germany). Subsequently, amplicons were restricted with an HhaI endonuclease (R0139L, New England Biolabs Inc., Ipswich, MA, USA). Finally, species-specific rDNA fragments were separated

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HhaI

HhaI from New England Biolabs

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HhaI from New England Biolabs

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