Glasgow's MEM (GMEM) from Thermo Fisher Scientific

Product Specs
ItemGlasgow's MEM (GMEM)
CompanyThermo Fisher Scientific
Price Pricing Info Get Pricing Get Quote for this Product
Catalog Number11710035
Quantity500 mL
ApplicationsCELL CULTURE
Brand/TrademarkGibco™
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals

Specifications/Features

Contains:
Contains L-glutamine, but no tryptose phosphate broth.

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Mosquito transgenerational antiviral immunity is mediated by vertical transfer of virus DNA sequences and RNAiiScienceJanuary 19, 2024Julio Rodriguez-Andres, Jason Axford, Ary Hoffmann, John Kneale Fazakerleyproteins" ,, TurboDNAse,Thermofisher,AM2238 Zidovudine,Sigma-Aldrich,1724500 Leibovitz’s L-15 medium,Gibco,11415064 DMEM,Gibco,11995-065 GMEM,Gibco,11710035 Blue dye 1,Merk,215643 Glutamax,Gibco,35050-061 GoTaq® Hot Start polymerase,Promega,M5005 Endonuclease

(2) Metabolic memory of Δ9-tetrahydrocannabinol exposure in pluripotent stem cells and primordial germ cells-like cellsElifeDecember 27, 2023Roxane Verdikt, Abigail A. Armstrong, Jenny Cheng, Young Sun Hwang, Amander T. Clark, Xia Yang, Patrick Allardlarge-scale induction (Nacalai) in the presence of GK15 medium (Glasgow’s Minimal Essential Medium [GMEM, 11710035, Thermo Fisher]) supplemented with 15% KSR, 0.1 mM Minimal Essential Medium Nonessential Amino Acids

(3) Internet-Connected Cortical Organoids for Project-Based Stem Cell and Neuroscience EducationeNeuroNovember 28, 2023Matthew A. T. Elliott, Hunter E. Schweiger, Ash Robbins, Samira Vera-Choqqueccota, Drew Ehrlich, Sebastian Hernandez, Kateryna Voitiuk, Jinghui Geng, Jess L. Sevetson, Cordero Core, et al.using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific #11710035), embryonic stem cell-qualified fetal bovine serum (Thermo Fisher Scientific #10439001), 0.1 mm MEM

(4) Internet-connected cortical organoids for project-based stem cell and neuroscience educationbioRxivJuly 20, 2023Matthew A.T. Elliott, Hunter E. Schweiger, Ash Robbins, Samira Vera-Choqqueccota, Drew Ehrlich, Sebastian Hernandez, Kateryna Voitiuk, Jinghui Geng, Jess L. Sevetson, Yohei M. Rosen, et al.using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001), 0.1 mM MEM

(5) Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmittersbioRxivJuly 19, 2023Yunjeong Park, Sebastian Hernandez, Cristian O. Hernandez, Hunter E. Schweiger, Houpu Li, Kateryna Voitiuk, Harika Dechiraju, Nico Hawthorne, Elana M. Muzzy, John A. Selberg, et al.using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001), 0.1 mM MEM

(6) Metabolic memory of Δ9-tetrahydrocannabinol exposure in pluripotent stem cells and primordial germ cells-like cellsbioRxivMarch 18, 2023Roxane Verdikt, Abigail A. Armstrong, Jenny Cheng, Xia Yang, Patrick Allardlarge-scale induction (Nacalai) in the presence of GK15 medium (Glasgow’s Minimal Essential Medium [GMEM, 11710035, Thermo Fisher] supplemented with 15% KSR, 0.1 mM Minimal Essential Medium Nonessential Amino Acids

(7) Structure of SALL4 zinc finger domain reveals link between AT-rich DNA binding and Okihiro syndromeLife Science AllianceMarch 1, 2023James A Watson, Raphaël Pantier, Uma Jayachandran, Kashyap Chhatbar, Beatrice Alexander-Howden, Valdeko Kruusvee, Michal Prendecki, Adrian Bird, Atlanta G CookCell culture Mouse ESCs (Hooper et al, 1987) were grown in Glasgow Minimum Essential Medium (GMEM; cat. 11710035; Thermo Fisher Scientific) supplemented with 15% FBS (batch tested), 1× L-glutamine (cat. 25030024;

(8) Comparing the transcriptome of developing native and iPSC-derived mouse retinae by single cell RNA sequencingScientific ReportsJanuary 21, 2023Anouk Georges, Arnaud Lavergne, Michiko Mandai, Fanny Lepiemme, Latifa Karim, Loic Demeulenaere, Diego Aguilar, Michael Schyns, Laurent Nguyen, Jean-Marie Rakic, et al.with 0.1% gelatin (G2625, Merck, Darmastadt, Germany) in Glasgow’s Minimum Essential Medium (GMEM, 11710035, Thermo Fisher Scientific (Fisher), Waltham, MA) supplemented with 10% Fetal Bovine Serum (FBS, 04-001-1,

(9) MitoQ Protects Ovarian Organoids against Oxidative Stress during Oogenesis and Folliculogenesis In VitroInternational journal of molecular sciencesJanuary 4, 2023Wang J, Du H, Ma L, Feng M, Li L, Zhao X, Dai Y.(from 1–3 dpp ovaries) in a U-bottomed 96-well plate for 2 d with GK15+RA medium (Glasgow MEM (Cat. No. 11710035, Thermo Fisher Scientific) supplemented with 15% Knockout serum replacement (Cat. No. 10828010, Thermo Fisher Scientific

(10) A simplified protocol for the generation of cortical brain organoidsFrontiers in cellular neuroscienceJanuary 1, 2023Eigenhuis KN, Somsen HB, van der Kroeg M, Smeenk H, Korporaal AL, Kushner SA, de Vrij FMS, van den Berg DLC.(ThermoFisher Scientific, 17502048);Heparin (Sigma, #H3149);Glasgow-MEM, GMEM (ThermoFisher Scientific, #11710035);MEM Non-Essential Amin Acids Solution (ThermoFisher Scientific, #11140050);DMEM/F-12, HEPES (ThermoFisher Scientific

(11) Robust induction of primordial germ cells of white rhinoceros on the brink of extinctionScience advancesDecember 9, 2022Hayashi M, Zywitza V, Naitou Y, Hamazaki N, Goeritz F, Hermes R, Holtze S, Lazzari G, Galli C, Stejskal J, et al.plate coated with fibronectin (Millipore, #FC010) and then cultured for 24 hours in GK15 [GMEM (Gibco, #11710035), 15% (v/v) KSR, 1× NEAA, 1× GlutaMAX, 1 mM sodium pyruvate (Gibco, #11360070), 0.1 mM 2-mercaptoethanol,

(12) Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCFNature CommunicationsDecember 1, 2022Abrar Aljahani, Peng Hua, Magdalena A. Karpinska, Kimberly Quililan, James O. J. Davies, A. Marieke Oudelaarplates pre-coated with 0.1% gelatin (Sigma-Aldrich, G1393) in Glasgow Modified Essential Medium (Gibco, 11710035) supplemented with 10% fetal bovine serum (Gibco, 10270106), 0.01 mM 2-Mercaptoethanol (Gibco, 31350010),

(13) PGCLCs of human 45,XO reveal pathogenetic pathways of neurocognitive and psychosocial disordersCell & bioscienceDecember 1, 2022Shang D, Lan T, Wang Y, Li X, Liu Q, Dong H, Xu B, Cheng H, Zhou R.hiPSCs were plated on 12-well plates (Sorfa, 220100) coated with matrigel in GK15 medium [GMEM (Gibco, 11710035) with 15% KnockOut™ Serum Replacement (Gibco, 10828028), 0.1 mM MEM-NEAA (Sigma, M7145-100 ml), 2 mM

(14) Prdm14 promotes mouse ESC self-renewal and PGCLC specification through enhancement of Stat3 activityiScienceNovember 18, 2022Li Y, Yang Z, Li X, Yu Y, Li X, Chen P, Li B, Wang X, Ye SD.(100 ng/mL, AF-250-03, Peprotech) and EGF (50 ng/mL, AF-100-15, Peprotech). GK15 consists of GMEM (11710035, Gibco), 15% KSR, 1×MEM nonessential amino acids, 0.1 mM β-mercaptoethanol and 1 mM sodium pyruvate

(15) Selective Induction of Intrinsic Apoptosis in Retinoblastoma Cells by Novel Cationic Antimicrobial DodecapeptidesPharmaceuticsNovember 18, 2022Vishnu Suresh Babu, Atish Kizhakeyil, Gagan Dudeja, Shyam S. Chaurasia, Veluchami Amutha Barathi, Stephane Heymans, Navin Kumar Verma, Rajamani Lakshminarayanan, Arkasubhra GhoshATCC, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F12 (cat#11710035, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (cat#A4766801, Gibco, Grand

(16) Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cellsSTAR ProtocolsSeptember 1, 2022Yukiko Ishikura, Hiroshi Ohta, Masahiro Nagano, Mitinori SaitouRecombinant",R&D,512GF Gelatin from bovine skin,Sigma-Aldrich,G9391 Glasgow’s MEM (GMEM),Thermo Fisher Scientific,11710035 GlutaMAX,Life Technologies,35050061 HEPES (1 M),Thermo Fisher Scientific,15630106 Insulin (powder),Sigma,"#I-1882,

(17) Robotic search for optimal cell culture in regenerative medicineeLifeJune 28, 2022Genki N Kanda, Taku Tsuzuki, Motoki Terada, Noriko Sakai, Naohiro Motozawa, Tomohiro Masuda, Mitsuhiro Nishida, Chihaya T Watanabe, Tatsuki Higashi, Shuhei A Horiguchi, et al.µg/mL streptomycin sulfate (#15140122, Thermo Fisher Scientific Inc, MA, USA). All diluted in GMEM (#11710035, Thermo Fisher Scientific Inc, MA, USA). RPE differentiation medium (15% KSR): 0.10 mM MEM NEAA (#11140050,

(18) Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cellsThe EMBO journalJune 14, 2022Severino J, Bauer M, Mattimoe T, Arecco N, Cozzuto L, Lorden P, Hamada N, Nosaka Y, Nagaoka SI, Audergon P, et al.Fisher Scientific, 81100525) at 2,000 cells per aggregate in GK15 medium (GMEM (Life Technologies, 11710035), 15% KnockOut Serum Replacement (KSR) (Thermo Fisher, 10828028), 0.1 mM nonessential amino acids (NEAA)

(19) Dissecting Human Gonadal Cell Lineage Specification and Sex Determination Using A Single-cell RNA-seq ApproachGenomics, Proteomics & BioinformaticsMay 2, 2022Rui Wang, Xixi Liu, Li Li, Ming Yang, Jun Yong, Fan Zhai, Lu Wen, Liying Yan, Jie Qiao, Fuchou Tangapproximately half of the cells were resuspended in Glasgow’s minimum essential medium (GMEM; Catalog No. 11710035, Gibco) supplemented with 15% (v/v) KnockOut Serum Replacement (Catalog No. 10828028, Gibco), 0.1 mM

(20) Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layersStem cell reportsMarch 8, 2022Kobayashi M, Kobayashi M, Odajima J, Shioda K, Hwang YS, Sasaki K, Chatterjee P, Kramme C, Kohman RE, Church GM, et al.sodium pyruvate, and 1× penicillin-streptomycin in Glasgow’s MEM with 2 mM glutamine (ThermoFisher, 11710035). STO-CM was prepared by maintaining 5.0 × 106 mitomycin C-treated STO cells in 12 mL of hPGCLC basal

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Glasgow's MEM (GMEM) from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Glasgow's MEM (GMEM) from Thermo Fisher Scientific

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