High Pure PCR Template Preparation Kit from Roche

Product Specs
ItemHigh Pure PCR Template Preparation Kit
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number11796828001
TimeSaves time by preparing multiple PCR templates simultaneously
MethodNucleic acids (NA) bind to the surface of glass fiber fleece in the presence of chaotropic salt. This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Quantity1 kit (100 purifications)
SourceBlood, Plasma/Serum, Cell/Tissue
Nucleic Acid TypeGenomic DNA

Specifications/Features

Starting Material:
Wide variety of sample materials (e.g ., whole blood, cells in culture, tissue)

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Draft genome assemblies of 12 Enterobacter hormaechei strains isolated from the North West province of South AfricaMicrobiology Resource AnnouncementsNovember 16, 2023Lerato Lisbeth Njaki Makhetha, Khayalethu Ntushelo, Baitsholetsi Gloria Mokolopi, James Wabwire Oguttu, Christian Anayochukwu Mbajiorgu, Goitsemang Makete, Tshifhiwa Paris Mamphogorocultures in BA using HighPure PCR template preparation kit (Roche Diagnostics, Mannheim, Germany; Cat No: 11796828001), following the manufacturer’s protocol. The extracted DNA concentration was measured using a NanoDrop

(2) Identification of ALK Mutation in Neuroblastoma on the Point of Molecular HeterogeneityTechnology in Cancer Research & TreatmentNovember 15, 2023Tekincan Çağrı Aktaş, Deniz Kızmazoğlu, Safiye Aktaş, Özde Elif Gökbayrak, Efe Serinan, Aylin Erol, Zekiye Altun, Hongling Yuan, Hatice Nur Olguneach pair of samples were used for DNA isolation, using the High Pure PCR Template Preparation Kit (11796828001; Roche) according to the manufacturer's instructions. DNA was quantified using a QubitTM dsDNA BR (Broad-Range)

(3) Heterozygosity of ALG9 in Association with Autosomal Dominant Polycystic Liver DiseaseGenes (Basel)September 2, 2023Melissa M. Boerrigter, Renée Duijzer, René H. M. te Morsche, Joost P. H. Drenthwhole blood samples, following the established protocol of the High Pure PCR Template Preparation Kit (11796828001, Roche Life Science, Penzberg, Germany). Subsequently, DNA underwent enrichment using the Twist Human Core Exome Kit

(4) A study implementing real-time PCR to identify Strongyloides species of third-stage larvae in human stool samples from Southern VietnamInfect Med (Beijing)September 1, 2023Le Duc Vinh, Nguyen Kim Thach, Huynh Hong Quang, Do Nhu Binh, Tran Thi Duc Hanh, Nguyen Minh Toàn, Nguyen Trung Tuyen, Nguyen Thu Huongsolution, and then used for DNA extraction by using the High Pure PCR Template Preparation Kit (Product No: 11796828001, Roche Life Science) in accordance with the manufacturer's instructions. The concentration and purity

(5) Novel α-1,3-Glucosyltransferase Variants and Their Broad Clinical Polycystic Liver Disease SpectrumGenes (Basel)August 19, 2023Melissa M. Boerrigter, René H. M. Te Morsche, Hanka Venselaar, Nikki Pastoors, Anja M. Geerts, Anne Hoorens, Joost P. H. Drenthwhole blood samples, according to the standard protocol of the High Pure PCR Template Preparation Kit (11796828001, Roche Life Science, Penzberg, Germany). The DNA concentration and quality (A260/A280 ratio: 1.80–1.99)

(6) ADAR1-mediated RNA editing promotes B cell lymphomagenesisiScienceJune 16, 2023Riccardo Pecori, Weicheng Ren, Mohammad Pirmoradian, Xianhuo Wang, Dongbing Liu, Mattias Berglund, Wei Li, Rafail Nikolaos Tasakis, Salvatore Di Giorgio, Xiaofei Ye, et al.mRNA Library Prep Kit,Illumina,Cat# RS-122-2101 High Pure PCR template Preparation kit,Roche,Cat# 11796828001 DNase,Invitrogen,Cat# AM1907 Q5® High-Fidelity DNA Polymerase,NEB,Cat# M0491 One-step RT-PCR kit,Qiagen,Cat#

(7) Trial of a Novel Oral Cannabinoid Formulation in Patients with Hypertension: A Double-Blind, Placebo-Controlled Pharmacogenetic StudyPharmaceuticals (Basel)April 25, 2023Ana Batinic, Davorka Sutlović, Sendi Kuret, Antonela Matana, Marko Kumric, Josko Bozic, Zeljko Dujiccommercial genomic DNA isolation kit (High Pure PCR Template Preparation Kit, Version 27, Cat. No. 11796828001, Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions. Extracted

(8) Investigation of the effects of mir-219-1 gene variants on the development of disease in non-small cell lung cancer patientsAfrican Health SciencesApril 24, 2023Sevgi Kalkanli Kalkanli Tas, Ender Coskunpinar, Pinar Yildiz, Mesut Bayraktaroğlu, Tuba Kose, Derya Altunkanat, Duygu Kirkik, Mustafa Tukenmezin venous blood. The manufacturer's instructions were followed for the DNA extraction (Product No: 11796828001 Roche Applied Sciences, Germany)18–20. After DNA isolation, the concentration and purity of the DNA

(9) Evaluation of Molecular Methods to Identify Chagas Disease and Leishmaniasis in Blood Donation Candidates in Two Brazilian CentersPathogensMarch 24, 2023Juliana de Jesus Guimarães Ferreira, Sandra Cecília Botelho Costa, Marcelo Addas-Carvalho, Mariane Barroso Pereira, Adriana de Oliveira França, Rodrigo Gonçalves de Lima, Paula Durante Andrade, Jamiro da Silva Wanderley, Luiz Cláudio Martins, Eros Antonio de Almeida, et al.using the High Pure PCR Template Preparation (Roche, Mannheim, Germany) commercial kit, code number 11796828001, following the manufacturer’s protocols. DNA was eluted in 200 uL of an elution buffer provided in the

(10) A pilot study using unique targeted testing of the urogenital microbiome has potential as a predictive test during IVF for implantation outcomeArchives of Gynecology and ObstetricsMarch 11, 2023Gloria E. Evans, Vishakha Mahajan, Sarah Wakeman, Tania Slatter, Anna P. Ponnampalam, Trevor P. Anderson, Makhdoom Sarwar, John J. Evansinstructions. Viral DNA was extracted from semen and FCU deposits using the High Pure PCR Template Preparation Kit #11796828001 (Roche) [27] according to the manufacturer’s instructions. qPCR setup The qPCR was set up according

(11) Influence of Selected Factors on the Survival Assessment and Detection of Giardia intestinalis DNA in Axenic CulturePathogensFebruary 14, 2023Małgorzata Smoguła, Roland Wesołowski, Marta Pawłowska, Celestyna Mila-Kierzenkowskain the open system: manual isolation kit (Roche, High Pure PCR Template Preparation Kit—catalog no. 11796828001); DNA Process Control Kit also containing reagents to prepare Master Mix reaction mix (Roche, DNA Process

(12) Assessment of the Association of Chlamydia e pneumoniae Infection with Lung Cancer in a Moroccan Patients’ CohortAsian Pacific Journal of Cancer PreventionFebruary 1, 2023Meryem Chebak, Maryame Azzouzi, Houda Chaibi, Meryem Fakhkhari, Ichrak Benamri, Malika Mguil, Khadija Hajjout, Adil Zegmout, Nabil Tiresse, Ismail Abderrahmane Rhorfi, et al.High pure isolation kit for genomic nucleic acid (Roche, High pure Pcr template preparation kit. USA 11796828001 ), following the manufacturer recommendations. Then, a nested Polymerase Chain Reaction (PCR) was performed

(13) The association between high‐risk human papillomavirus and oral lichen planusClinical and Experimental Dental ResearchFebruary 1, 2023Maryam Mohammadi, Hamid Abbaszadeh, Nooshin Mohtasham, Hamid Salehiniya, Ebrahim Shafaiesamples was extracted with a high pure polymerase chain reaction (PCR) template preparation kit (Cat. no: 11796828001, Roche Diagnostics GmbH, Penzberg, Germany), according to the manufacturer's instructions for FFPE tissue

(14) Breast cancer in East Africa: Prevalence and spectrum of germline SNV /indel and CNVs in BRCA1 and BRCA2 genes among breast cancer patients in TanzaniaCancer MedicineFebruary 1, 2023Linus P. Rweyemamu, Büşra K. Gültaşlar, Gokce Akan, Nazima Dharsee, Lucy A. Namkinga, Sylvester L. Lyantagaye, Hülya Yazıcı, Fatmahan AtalarGenomic DNA was manually extracted from blood leucocytes using High Pure PCR Template Preparation Kit no. 11796828001 (Roche Life Science) following manufacturer's recommendations. The DNA integrity was assessed by running

(15) ADAR RNA editing on antisense RNAs results in apparent U-to-C base changes on overlapping sense transcriptsFrontiers in Cell and Developmental BiologyJanuary 6, 2023Riccardo Pecori, Isabel Chillón, Claudio Lo Giudice, Annette Arnold, Sandra Wüst, Marco Binder, Marco Marcia, Ernesto Picardi, Fotini Nina PapavasiliouDNA (gDNA) and RNA. gDNA was extracted using the High Pure PCR Template Preparation kit (Roche, Cat# 11796828001) following manufacturer instructions. PCR amplification was performed using Q5® High-Fidelity DNA Polymerase

(16) Extensive proteome and functional genomic profiling of variability between genetically identical human B-lymphoblastoid cellsScientific DataDecember 10, 2022Miklós Laczik, Edina Erdős, Lilla Ozgyin, Zsuzsanna Hevessy, Éva Csősz, Gergő Kalló, Tibor Nagy, Endre Barta, Szilárd Póliska, István Szatmári, et al.penicillin-streptomycin. Genomic DNA was isolated using a High Pure PCR Template Preparation Kit (Roche Life Science, cat. 11796828001). Library preparation was performed by Novogene Co., Ltd. from 1 microgram of DNA per sample based on

(17) Synthetic lethality between TP53 and ENDOD1Nature CommunicationsDecember 1, 2022Zizhi Tang et al.digestion Genomic DNA was extracted from 106 cells using High Pure PCR Template Preparation Kit (Roche, 11796828001). 150 ng of purified DNA was digested in Reaction Buffer (200 mM sodium acetate [pH 4.5], 1.5 M NaCl

(18) Nuclear corepressors NCOR1/NCOR2 regulate B cell development, maintain genomic integrity, and prevent transformationNature ImmunologyDecember 1, 2022Robin D. Lee, Todd P. Knutson, Sarah A. Munro, Jeffrey T. Miller, Lynn M. Heltemes-Harris, Charles G. Mullighan, Kristen Jepsen, Michael A. Farrarcells from each sample; CD19+B220+ B cells were subject to DNA isolation using a DNA extraction kit (11796828001, Roche). Nucleic acid quantification was performed using both a nanodrop (ND-ONE-W, ThermoFisher) and

(19) Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancersNature CommunicationsDecember 1, 2022Renata Bordeira-Carriço et al.(Supplementary Dataset 4c). Final PCR products were purified with the High Pure PCR Product Purification Kit (#11796828001, Roche) and AMPure XP PCR purification kit (#B37419AB, Agencourt AMPure XP). HiChIP-seq HiChIP-seq

(20) IRF4 deficiency vulnerates B-cell progeny for leukemogenesis via somatically acquired Jak3 mutations conferring IL-7 hypersensitivityCell death and differentiationNovember 1, 2022Das Gupta D, Paul C, Samel N, Bieringer M, Staudenraus D, Marini F, Raifer H, Menke L, Hansal L, Camara B, et al.FACS-sorted control B220+sIgµ− BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). The integrity of the resultant gDNA was confirmed in a 2% Agarose gel. Macrogen in Seoul performed

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High Pure PCR Template Preparation Kit from Roche

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Specifications/Features

Roche

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High Pure PCR Template Preparation Kit from Roche

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