MACSxpress Separator from Miltenyi Biotec

(1) The Candida albicans ζ-crystallin homolog Zta1 promotes resistance to oxidative stressmSphereDecember 20, 2023Rafael M. Gandra, Chad J. Johnson, Jeniel E. Nett, James B. Konopkaof Wisconsin Internal Review Board. Neutrophils were isolated from four different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and

(2) Phagocytic cell death leads to enhanced release of pro-inflammatory S100A12 in familial Mediterranean feverMol Cell PediatrDecember 13, 2023G. Varga, S. Schleifenbaum, U. Koenig, J. Waldkirch, C. Hinze, C. Kessel, W. Geluk, T. Pap, Elke Lainka, Tilmann Kallinich, et al.and subsequent stimulation and inhibition Neutrophils were isolated from whole blood (EDTA) using MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the

(3) ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysisNat CommunDecember 11, 2023Zülal Cibir, Jacqueline Hassel, Justin Sonneck, Lennart Kowitz, Alexander Beer, Andreas Kraus, Gabriel Hallekamp, Martin Rosenkranz, Pascal Raffelberg, Sven Olfen, et al.neutrophils were isolated from 1 ml EDTA-supplemented blood using magnetic negative isolation with the MACSxpress® Whole Blood Neutrophil Isolation Kit (Cat. No.: 130-104-434, Miltenyi Biotec, Bergisch Gladbach, Germany)

(4) Activation induces shift in nutrient utilization that differentially impacts cell functions in human neutrophilsbioRxivNovember 2, 2023Emily C. Britt, Xin Qing, James A. Votava, Jorgo Lika, Andrew Wagner, Simone Shen, Nicholas L. Arp, Hamidullah Khan, Stefan M. Schieke, Christopher D. Fletcher, et al.2019-1031-CP001). Informed consent was obtained from all participants. Neutrophils were purified using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, no. 130-104-434) followed by erythrocyte depletion

(5) CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signalingFrontiers in ImmunologyOctober 24, 2023Sean P. Giblin, Sashini Ranawana, Shyreen Hassibi, Holly L. Birchenough, Kyle T. Mincham, Robert J. Snelgrove, Tomoko Tsuchiya, Shiro Kanegasaki, Douglas Dyer, James E. Peasefrom healthy donor whole blood samples obtained with local ethical approval. Purification used the MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi) according to the manufacturer’s instructions. Prior

(6) Neutralization, effector function and immune imprinting of Omicron variantsNatureSeptember 1, 2023Addetia A, Piccoli L, Case JB, Park YJ, Beltramello M, Guarino B, Dang H, de Melo GD, Pinto D, Sprouse K, et al.BA.2-E340A) as target cells. Natural killer cells were isolated from fresh blood of healthy donors using the MACSxpress WB NK cell isolation kit, human (Miltenyi Biotec, 130-127-695). Target cells were incubated with titrated

(7) iCLOTS: open-source, artificial intelligence-enabled software for analyses of blood cells in microfluidic and microscopy-based assaysNat CommunAugust 18, 2023Meredith E. Fay, Oluwamayokun Oshinowo, Elizabeth Iffrig, Kirby S. Fibben, Christina Caruso, Scott Hansen, Jamie O. Musick, José M. Valdez, Sally S. Azer, Robert G. Mannino, et al.isolated from whole blood collected in EDTA using a whole blood human neutrophil isolation kit (Miltenyi MACSxpress) and resuspended in PBS. Transient neutrophil adhesion assay 0.1 M N-Formylmethionyl-leucyl-phenylalanine

(8) Heterodimerization of Chemoreceptors TAS1R3 and mGlu2 in Human Blood LeukocytesInt J Mol SciAugust 18, 2023Lena Ball, Julia Bauer, Dietmar Krautwurstwere isolated from buffy coat samples (Health Center Dr. Becker, Munich, Germany) and purified using MACSxpress® Whole Blood Neutrophil Isolation Kit or Pan T Cell Isolation Kit, human, respectively (Miltenyi Biotec,

(9) Effects of GLP-1 receptor agonists on mitochondrial function, inflammatory markers and leukocyte-endothelium interactions in type 2 diabetesRedox BiolAugust 14, 2023Clara Luna-Marco, Arantxa M. de Marañon, Alberto Hermo-Argibay, Yohaly Rodriguez-Hernandez, Jonathan Hermenejildo, Meylin Fernandez-Reyes, Nadezda Apostolova, Jose Vila, Eva Sola, Carlos Morillas, et al.obtained from whole blood collected in EDTA-coated tubes from all subjects. Isolation was performed with a MACSxpress® Whole Blood Starting Kit (Miltenyi Biotec) following the manufacturer’s guidelines. 2.3 Flow cytometry Whole

(10) Human Neutrophil Response to Pseudomonas Bacteriophage PAK_P1, a Therapeutic CandidateVirusesAugust 12, 2023Dwayne R. Roach, Benoît Noël, Sylvie Chollet-Martin, Mathieu de Jode, Vanessa Granger, Laurent Debarbieux, Luc de Chaisemartinseparated from human peripheral blood collected from healthy donors on EDTA by negative magnetic sorting (MACSxpress, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. Neutrophils

(11) Single-cell analysis of lizard blastema fibroblasts reveals phagocyte-dependent activation of Hedgehog-responsive chondrogenesisNat CommunAugust 10, 2023Ariel C. Vonk, Xiaofan Zhao, Zheyu Pan, Megan L. Hudnall, Conrad G. Oakes, Gabriela A. Lopez, Sarah C. Hasel-Kolossa, Alexander W. C. Kuncz, Sasha B. Sengelmann, Darian J. Gamble, et al.DNase I type IV in Leibovitz’s L15 medium). Erythrocytes were depleted using the magnetic bead-based MACSxpress® Erythrocyte Depletion Kit (Miltenyi Biotec, PN:130-098-196) according to the manufacturer’s instructions.

(12) A pan-influenza antibody inhibiting neuraminidase via receptor mimicryNatureJune 15, 2023Corey Momont, Ha V. Dang, Fabrizia Zatta, Kevin Hauser, Caihong Wang, Julia di Iulio, Andrea Minola, Nadine Czudnochowski, Anna De Marco, Kaitlin Branch, et al.10 min at room temperature. Human natural killer (NK) cells were isolated from fresh blood using the MACSxpress WB NK cell isolation kit, human (130-127-695, Miltenyi Biotec) and following the manufacturer’s instructions.

(13) Targeting the Granulocytic Defense against A. fumigatus in Healthy Volunteers and Septic PatientsInternational Journal of Molecular SciencesJune 8, 2023Stefanie Michel, Lisa Kirchhoff, Peter-Michael Rath, Jansje Schwab, Karsten Schmidt, Thorsten Brenner, Simon Dublerthrough changes in surface proteins [42]. 4.4. Isolation of NG The human NGs were isolated with a MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the

(14) An integrated cell atlas of the lung in health and diseaseNature MedicineJune 8, 2023Lisa Sikkema, Ciro Ramírez-Suástegui, Daniel C. Strobl, Tessa E. Gillett, Luke Zappia, Elo Madissoon, Nikolay S. Markov, Laure-Emmanuelle Zaragosi, Yuge Ji, Meshal Ansari, et al.cells were filtered over a 70 µm cell strainer (BD Falcon). Erythrocytes were removed using a human MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec) and cells were filtered using a 40 µm cell strainer (BD

(15) Increasing human monoclonal antibody cloning efficiency with a whole-cell modified immunoglobulin-capture assay (mICA)Frontiers in ImmunologyJune 2, 2023Sara Siris, Camilla A. Gladstone, Yanping Guo, Radhika Patel, Christopher L. Pinder, Robin J. Shattock, Paul F. McKay, Paul R. Langford, Fadil A. Bidmoskilling assay Neutrophils were purified from freshly-obtained blood of healthy human donors using the MACSxpress magnetic purification system (Miltenyi Biotechnology, cat. No. 130-104-434), as per manufacturer’s instructions.

(16) Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periodsCell Reports MethodsJune 1, 2023Fabian Otte, Yuepeng Zhang, Julian Spagnuolo, Alexander Thielen, Martin Däumer, Carsten Wiethe, Marcel Stoeckle, Katharina Kusejko, Florian Klein, Karin J. Metzner, et al.,STEMCELL Technologies,Cat#: 10981 ImmunoCult™ Human CD3/CD28/CD2 T cell Activator,STEMCELL Technologies,Cat#: 10970 "MACSxpress® Whole Blood CD4 T cell Isolation Kit, human",Miltenyi Biotec,Cat#: 130-098-195 Zero Blunt™ TOPO™ PCR Cloning

(17) The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivoResearch SquareMay 10, 2023Chao Li, Mehtab Farooqui, Ravi Chandra Yada, Joseph B. Cai, Anna Huttenlocher, David J. Beebeisolated from whole blood using magnetic bead-based negative selection. Two negative selection kits - MACSxpress Whole Blood Neutrophil Isolation Kit, human (Miltenyi Biotec, 130-104-434) and EasySep Direct Human Neutrophil Isolation Kit

(18) TLR4-mediated release of heparin-binding protein in human airways: a co-stimulatory role for IL-26Frontiers in ImmunologyMay 10, 2023Magnus Paulsson, Eduardo I. Cardenas, Karlhans F. Che, Bettina Brundin, Margaretha Smith, Ingemar Qvarfordt, Anders LindénBlood Donor Center (Karolinska University Hospital, Solna) and neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). In brief, 8 ml of

(19) Leptin deficiency in CD8+ T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme BFrontiers in ImmunologyMay 3, 2023Meiyu Wu, Lu Wang, Haijing Wu, Ming Yang, Zhenghao He, Yiran Chen, Huiming Zhangisolate the CD8+ T cells. CD8+ T cells were isolated from spleen samples of Lep KO and WT mouses using MACSxpress® whole blood CD8 T cell isolation kit mouse (Miltenyi Biotec, Auburn, CA). All the non-CD8 T cells were

(20) Antibodies against Poly(ethylene glycol) Activate Innate Immune Cells and Induce Hypersensitivity Reactions to PEGylated NanomedicinesACS NanoMarch 28, 2023Wei-An Chen, Deng-Yuan Chang, Bing-Mae Chen, Yi-Chen Lin, Yechezekel Barenholz, Steve R. RofflerEncapsula NanoSciences (Brentwood, TN, USA). MidiMacs separation columns, MidiMacs magnetic columns, and MACSxpress separator were from Miltenyi Biotec (Bergisch Gladbach, Germany). ELISA kits for histamine were from LDN (Nordhorn,

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5/11/2016A Solution for Isolation of Magnetized Cells Jesús CirizaWe used this kit for isolation of magnetized cells; we were able to isolate magnetized cells from a cell culture as can be observed in the figure.

MACSxpress Separator

MACSxpress Separator from Miltenyi Biotec

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Miltenyi Biotec

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MACSxpress Separator from Miltenyi Biotec

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