Endonuclease IV from New England Biolabs

Product Specs
ItemEndonuclease IV
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0304S
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 µl in 1 hour at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Novel mechanisms for the removal of strong replication-blocking HMCES- and thiazolidine-DNA adducts in humansNucleic Acids ResearchJune 9, 2023Yohei Sugimoto, Yuji Masuda, Shigenori Iwai, Yumi Miyake, Rie Kanao, Chikahide Masutani(EndoIII), and endonuclease IV (EndoIV) were purchased from New England Biolabs (M0280L, M0268S and M0304S, respectively). HMCES-his was purified as follows. E. coli BL21 (DE3) harbouring pET20-HMCES-his and

(2) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.consists of four steps. In step 1, DNA is treated with an enzyme cocktail consisting of EndoIV (NEB, M0304S), Fpg (NEB, M0240S), UDG (NEB, M0280S), T4 PDG (NEB, M0308S), EndoVIII (NEB, M0299S) and ExoVII (NEB,

(3) DNA-protein cross-links between abasic DNA damage and mitochondrial transcription factor A (TFAM)Nucleic acids researchJanuary 11, 2023Xu W, Tang J, Zhao L.reaction or Endo III reaction was performed by incubating the reaction mixture with 10 units endo IV (NEB, M0304S) and 1 mM DTT at 37°C for 1 h. Reactions of AP-DNA with endo IV were with 4 μM AP-DNA, 10 units endo

(4) Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution while enabling target enrichmentGenome ResearchNovember 4, 2022Bo Yan, Duan Wang, Romualdas Vaisvila, Zhiyi Sun, Laurence Ettwillerpositions in the hairpin adapters at both ends after being treated with UDG (NEB, M0280) and EndoVI (NEB, M0304). The nick sites were translated toward 3′ terminus by DNA polymerase I (NEB, M0209) in the presence

(5) Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP methodNature CommunicationsOctober 5, 2022Ye Cai, Huifen Cao, Fang Wang, Yufei Zhang, Philipp KapranovDTT) and resuspended in a 20 µl reaction system including 20 U Endonuclease IV (New England BioLabs, M0304S) and 1× NEBuffer 3 followed by 2 h incubation at 37 °C. Library construction and sequencing The purified

(6) Highly multiplexed selection of RNA aptamers against a small molecule libraryPLOS ONESeptember 15, 2022Brent Townshend, Matias Kaplan, Christina D. Smolkereaction consisted of 0.05U/μl Uracil-DNA Glycosylase (NEB; M0280), 0.1 U/μl Endonuclease IV (NEB; M0304), 1x ThermoPol buffer (NEB; B9004), and 2 μl of a diluted CleaveSeq reaction in a total volume of 10

(7) Repair-Assisted Damage Detection Reveals Biological Disparities in Prostate Cancer between African Americans and European AmericansCancersFebruary 17, 2022Krieger KL, Gohlke JH, Lee KJ, Piyarathna DWB, Castro PD, Jones JA, Ittmann MM, Gassman NR, Sreekumar A.PDG (NEB M0308),5 U,Thermo Pol Buffer,10 µL T4 PDG (NEB M0308),5 U,10 µL,(NEB B9004) EndoIV (NEB M0304),5 U,, AAG (NEB M0313),5 U,, "NAD+ (100x, NEB B9007)",500 µM,, "BSA (Sigma Aldrich, St. Louis, MO,

(8) DNA-Protein Cross-Linking Sequencing for Genome-Wide Mapping of Thymidine GlycolJournal of the American Chemical SocietyJanuary 12, 2022Tang F, Yuan J, Yuan BF, Wang Y.removed by incubating the isolated DNA with an enzyme cocktail containing 1 μL of endonuclease IV (NEB, M0304), 1 μL of Fpg (NEB, M0240S), 1 μL of hSMUG1 (NEB, M0336S) in a buffer containing 25 mM Tris (pH 7.5),

(9) Duplex-Repair enables highly accurate sequencing, despite DNA damageNucleic Acids ResearchJanuary 11, 2022Kan Xiong, Douglas Shea, Justin Rhoades, Timothy Blewett, Ruolin Liu, Jin H Bae, Erica Nguyen, G Mike Makrigiorgos, Todd R Golub, Viktor A Adalsteinssonconsists of four steps. In step 1, DNA is treated with an enzyme cocktail consisting of EndoIV (Cat. No. M0304S), Fpg (Cat. No. M0240S), UDG (Cat. No. M0280S), T4 PDG (Cat. No. M0308S), EndoVIII (Cat. No. M0299S)

(10) Click and Cut: a click chemistry approach to developing oxidative DNA damaging agentsNucleic Acids ResearchOctober 11, 2021Natasha McStay, Creina Slator, Vandana Singh, Alex Gibney, Fredrik Westerlund, Andrew KellettCutSmart buffers, pUC19 plasmid (N3041) and repair enzymes—Fpg (M0240S), Endo III (M0268S), Endo IV (M0304S), Endo V (M0305S), hAAG (M0313S) and APE1 (M0282S)—were purchased from New England Biolabs. Aminoallyl-dUTP-ATTO-647N

(11) Endonuclease enrichment TAPS for cost-effective genome-wide base-resolution DNA methylation detectionNucleic Acids ResearchJuly 21, 2021Jingfei Cheng, Paulina Siejka-Zielińska, Yibin Liu, Anandhakumar Chandran, Skirmantas Kriaucionis, Chun-Xiao Songmanufacturers’ protocols (all from New England Biolabs): USER (Cat. No. M5505S), Endonuclease IV (Cat. No. M0304S), Tma Endonuclease III (Cat. No. M0291S), Endonuclease V (Cat. No. M0305S), UDG (Cat. No. M0280S), Tth Endonuclease IV

(12) Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg ProteinFrontiers in MicrobiologyJune 30, 2021Almaz Nigatu Tesfahun, Marina Alexeeva, Miglė Tomkuvienė, Aysha Arshad, Prashanna Guragain, Arne Klungland, Saulius Klimašauskas, Peter Ruoff, Svein Bjelland(EDTA), 200 μg/mL BSA, 50% glycerol], Ung [Cat. No. M0280S; 5,000 U/mL (1.95 pmol/μL)], Nfo [Cat. No. M0304S; 10,000 U/mL (83 nM)], and polynucleotide kinase (PseT) [Cat. No. M0201S; 10,000 U/mL (0.29 μM)] were

(13) Associations between DNA Damage and PD-L1 Expression in Ovarian Cancer, a Potential Biomarker for Clinical ResponseBiology (Basel)April 29, 2021Elise K. Mann, Kevin J. Lee, Dongquan Chen, Luciana Madeira da Silva, Valeria L. Dal Zotto, Jennifer Scalici, Natalie R. Gassman(Sigma Aldrich 11093088910) 0.1 T4 PDG (NEB M0308) 5U Thermo Pol Buffer (NEB B9004) 10 µL EndoIV (NEB M0304) 5U EndoVIII (NEB M0299) 5U AAG (NEB M0313) 5U NAD+ (100×, NEB B9007) 500 µM BSA (Sigma Aldrich,

(14) A low-bias and sensitive small RNA library preparation method using randomized splint ligationNucleic Acids ResearchAugust 20, 2020Sean Maguire, Gregory J S Lohman, Shengxi Guanand 5 min at 75°C. Five units of Uracil-DNA Glycosylase (NEB M0280) and 20 units of Endonuclease IV (M0304) were added and reactions were incubated for an additional hour at 37°C. The 5′ ligation was then performed

(15) DNA damage measurements within tissue samples with Repair Assisted Damage Detection (RADD)Current research in biotechnologyNovember 1, 2019Lee KJ, Mann E, da Silva LM, Scalici J, Gassman NR.Aldrich 11573152910),1 μM T4PDG (NEB M0308S),5 U,Therm Pol buffer (NEB B9004S),10 μL EndoIV (NEB M0304L),5 U,, hAAG (NEBM0313S),5 U,, "NAD+ (100x, NEB B9007S)",500 μM,, BSA (Sigma Aldrich),200 μg/mL,, Therm

(16) Defective base excision repair in the response to DNA damaging agents in triple negative breast cancerPloS oneJanuary 1, 2019Lee KJ, Piett CG, Andrews JF, Mann E, Nagel ZD, Gassman NR.After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions,

(17) Broad spectrum detection of DNA damage by Repair Assisted Damage Detection (RADD)DNA repairJune 1, 2018Holton NW, Ebenstein Y, Gassman NR.contains enzymes purchased from NEB (UDG #M0280S, Fapy-DNA glycosylase # M0240S, T4PDG #M0308S, Endo IV # M0304S, Endo VIII # M0299S) and prepared in 1x Thermpol buffer (NEB # B9004S) and incubated at 37°C in a humidified

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Endonuclease IV

Endonuclease IV from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (17)

Reviews

Endonuclease IV from New England Biolabs

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