Monarch PCR & DNA Cleanup Kit (5 g) from New England Biolabs

Product Specs
ItemMonarch PCR & DNA Cleanup Kit (5 g)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberT1030S
Quantity50 preps
ApplicationsDNA Manipulation
SourceReaction
Nucleic Acid TypeDNA Fragment, PCR Product
Temperature25 C
Brand/TrademarkNew England Biolabs Inc (NEB)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Heterologously expressed SacP23, a novel bacteriocin from Paenibacillus polymyxa #23, is active against methicillin resistant Staphylococcus aureusR Soc Open SciDecember 20, 2023Châu Minh Khánh, Dong Van Quyen, Thi Thu Hao Van, Robert J. Mooreextension at 72°C for 10 min. The PCR product was purified using the Monarch PCR & DNA Cleanup Kit (T1030S, New England Biolabs). The expression plasmid was generated by fusing the vector plasmid backbone, pDLL202,

(2) Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organellesbioRxivNovember 30, 2023Leonid Andronov, Mengting Han, Yanyu Zhu, Anish R. Roy, Andrew E. S. Barentine, Jaishree Garhyan, Lei S. Qi, W.E. Moernerthe dark for conjugation and purified for 3 rounds using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions. The estimated labeling efficiency of probes was calculated

(3) HOXDeRNA activates a cancerous transcription program and super-enhancers genome-widebioRxivNovember 24, 2023Evgeny Deforzh, Prakash Kharel, Anton Karelin, Pavel Ivanov, Anna M. Krichevsky(SimpleChIP® Enzymatic Chromatin IP Kit). DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB) and ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

(4) Soil as a transdisciplinary research catalyst: from bioprospecting to biorespectingRoyal Society Open ScienceNovember 15, 2023Matthew J. Tarnowski, Gilda Varliero, Jim Scown, Emily Phelps, Thomas E. Gorochowskiby a final extension (72°C, 10 min). Samples were purified using Monarch PCR & DNA cleanup kit (NEB T1030S), with elution in nuclease-free water (20 µl). PCR amplicons were checked by gel electrophoresis (1

(5) The genetics of aerotolerant growth in an alphaproteobacterium with a naturally reduced genomemBioOctober 31, 2023Amy L. Enright, Amy B. Banta, Ryan D. Ward, Julio Rivera Vazquez, Magdalena M. Felczak, Michael B. Wolfe, Michaela A. TerAvest, Daniel Amador-Noguez, Jason M. PetersDNA polymerase (NEB 0491). PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030). Plasmids were transformed into electrocompetent E. coli cells using a Bio-Rad Gene Pulser Xcell using

(6) The SMN complex drives structural changes in human snRNAs to enable snRNP assemblyNature CommunicationsOctober 18, 2023Josef Pánek, Adriana Roithová, Nenad Radivojević, Michal Sýkora, Archana Bairavasundaram Prusty, Nicholas Huston, Han Wan, Anna Marie Pyle, Utz Fischer, David StaněkNo. M0544L). PCR reaction products were cleaned using Monarch PCR&DNA Clean-up Kits (NEB, Cat. No. T1030S) and a binding buffer:sample ratio of 5:1. Remaining Illumina adapter sequences were added using the

(7) Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activationDevelopmental CellOctober 9, 2023Kaelan J. Brennan, Melanie Weilert, Sabrina Krueger, Anusri Pampari, Hsiao-yun Liu, Ally W.H. Yang, Jason A. Morrison, Timothy R. Hughes, Christine A. Rushlow, Anshul Kundaje, et al.Monarch DNA Gel Extraction Kit,New England Biolabs,Cat# T1020 Monarch PCR & DNA Cleanup Kit,New England Biolabs,Cat# T1030 PURExpress In Vitro Protein Synthesis Kit,New England Biolabs,Cat# E6800 Hybridization Chain Reaction

(8) Discrepancies in indel software resolution with somatic CRISPR/Cas9 tumorigenesis modelsScientific ReportsSeptember 8, 2023Qierra R. Brockman, Amanda Scherer, Gavin R. McGivney, Wade R. Gutierrez, Jeffrey Rytlewski, Alexa Sheehan, Akshaya Warrier, Emily A. Laverty, Grace Roughton, Nina C. Carnevale, et al.our previous publication20. PCR amplicons were purified with the Monarch PCR and DNA Cleanup Kit (NEB T1030S). Sanger sequencing was performed by the Genomics Division of the Iowa Institute of Human Genetics at

(9) Gene Replacement by a Selectable Marker in the Filamentous Fungus Magnaporthe oryzaeBIO-PROTOCOLSeptember 5, 2023Nalleli Garcia, Alexa N. Farmer, Richmond Baptiste, Jessie Fernandezfilters (Fisher Scientific, catalog number: 09-720-514)Monarch PCR purification kit (NEB, catalog number: T1030S) Equipment Incubator (Percival, model: I36VL)Centrifuge (Eppendorf, model: Centrifuge 5810 R with

(10) Characterization of microRNAs in the cyst nematode Heterodera glycines identifies possible candidates involved in cross-kingdom interactions with its host Glycine maxRNA BiolAugust 21, 2023Dave T. Ste-Croix, Richard R. Bélanger, Benjamin Mimeewere individually purified into a final volume of 25 µL using the NEB Monarch PCR & DNA cleanup kit (T1030, NEB – Ipswich, Massachusetts, United States). A ratio of 1.3× Mag-Bind® TotalPure NGS beads (M1378–01,

(11) KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumorsFrontiers in ImmunologyAugust 21, 2023Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay, Hugo Roméro, Yuanyi Li, Renaud Dion, Kathie Béland, et al.5′-AGCTTCTCTGGAGCTGATGG-3′ and purified using Monarch® PCR & DNA Cleanup Kit (NEB, Ipswich, MA, USA; T1030S). Genome editing efficiency was assessed using the T7 endonuclease I assay (T7E1; NEB, M0302S), and

(12) Enhancing CRISPR prime editing by reducing misfolded pegRNA interactionsbioRxivAugust 20, 2023Weiting Zhang, Karl Petri, Junyan Ma, Hyunho Lee, Chia-Lun Tsai, J. Keith Joung, Jing-Ruey Joanna Yehmin. The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit

(13) Expression conditions and characterization of a novelly constructed lipoprotein intended as a vaccine to prevent human Haemophilus influenzae infectionsJournal of Biological ChemistryAugust 1, 2023Ravinder Kaur, Jill Mangiafesto, Karin Pryharski, Sailee Rasam, Robert Zagursky, Michael PichicheroTechnologies. PCR reaction components were removed using the Monarch PCR & DNA cleanup kit (catalog # T1030S, NEB), and the DNA was digested with EcoR1 and Sca1 (NEB). Both the digested lnt gene and pACYC184 DNA

(14) oFlowSeq: a quantitative approach to identify protein coding mutations affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoidsHuman geneticsAugust 1, 2023Dawes P, Murray LF, Olson MN, Barton NJ, Smullen M, Suresh M, Yan G, Zhang Y, Fernandez-Fontaine A, English J, et al.Nextera indexes, followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems

(15) Characterisation of a Live-Attenuated Rabies Virus Expressing a Secreted scFv for the Treatment of RabiesVirusesJuly 31, 2023Samuel P. Smith, Rebecca Shipley, Pascal Drake, Anthony R. Fooks, Julian Ma, Ashley C. Banyardisolated using either NEB Monarch® DNA Gel Extraction Kit (T1020L) or NEB Monarch® PCR & DNA Cleanup Kit (T1030L). To generate cDNA from viral RNA extraction, the QIAGEN OneStep RT-PCR Kit was used (Qiagen, Hilden,

(16) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.Dual-Luciferase Reporter Assay System,Promega,Cat#E1910 DNeasy Blood and Tissue kit,Qiagen,Cat#69506 Monarch PCR & DNA Cleanup Kit,NEB,Cat#T1030L TruSeq Stranded Total RNA Ribo-Zero Gold,Illumina,Cat#RS-122-2301 ,, Deposited data,Deposited data,Deposited

(17) Centromere repositioning and shifts in wheat evolutionPlant CommunicationsJuly 10, 2023Jing Zhao, Yilin Xie, Chuizheng Kong, Zefu Lu, Haiyan Jia, Zhengqiang Ma, Yijing Zhang, Dangqun Cui, Zhengang Ru, Yuquan Wang, et al.for 30 min without rotation. The integration products were purified using a Monarch DNA Cleanup Kit (T1030L, New England Biolabs) and then amplified using Phusion DNA polymerase for 10–13 cycles. The PCR cycles

(18) Genome analysis of Cephalotrichum gorgonifer and identification of the biosynthetic pathway for rasfonin, an inhibitor of KRAS dependent cancerFungal Biology and BiotechnologyJune 24, 2023Andreas Schüller, Lena Studt-Reinhold, Harald Berger, Lucia Silvestrini, Roman Labuda, Ulrich Güldener, Markus Gorfer, Markus Bacher, Maria Doppler, Erika Gasparotto, et al.cloning (YRC) [90]. All PCR fragments were purified with the Monarch PCR & DNA Cleanup Kit (NEB; Art. No. T1030L), measured at a NanoDrop™ spectrophotometer (Thermo Fisher Scientific™; Art. No. ND-2000C). All reactions

(19) Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxivJune 19, 2023Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. KaplanQ5 high-fidelity DNA polymerase (NEB #M0494S, Ipswich, MA, USA), DpnI digestion (NEB #R0176S), PCR cleanup (NEB #T1030S), assembly with the NEBuilder HiFi kit (NEB #E2621S), and heat-shock transformation into competent E. coli

(20) Fertility decline in female mosquitoes is regulated by the orco olfactory co-receptoriScienceJune 16, 2023Olayinka G. David, Kevin M. Sanchez, Andrea V. Arce, Andre Luis Costa-da-Silva, Anthony J. Bellantuono, Matthew DeGennaro# 69504 RNeasy kit,QIAGEN,Cat. # 75144 Monarch PCR and DNA Cleanup kit,New England BioLabs,Cat. # T1030L GoTaq Green Master Mix,Promega Corporation,Cat. # M7123 Qubit dsDNA BR Assay Kit,Invitrogen,Cat. #

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(1)

11/3/2020High-Quality DNA Purification Xuewei WangRecommended for PCR and DNA purification.
(1)

6/15/2020Purification of PCR DNA Samples for SequencingDr. Thippeswamy SannasiddappaIt is a good kit but not as good as other leading providers such Qiagen.
(1)

2/24/2017Excellent DNA Clean Up Kit Soledad Miranda-RottmannAppealing format (small box, small bottles, clear labels, sort of eco-friendly) and reliable good results. The yield could be improved: it is approx 50%, but since the elution volume is only 6 ul, ... Read Review