Macro-Prep High Q Support from Bio-Rad

Product Specs
ItemMacro-Prep High Q Support
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1580040
Brand/TrademarkLSG PFTL

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution proteineLifeNovember 23, 2022Kayla A Jara, Nikolaus M Loening, Patrick N Reardon, Zhen Yu, Prajna Woonnimani, Coban Brooks, Cat H Vesely, Elisar J BarbarSciences, Pittsburgh, PA) SEC column and then, for IC1-260 samples, followed by anion exchange using Macro-Prep High Q Support resin (Bio-Rad, Hercules, California) with elution in 0.1–0.2 M sodium chloride. Protein concentrations

(2) Shelterin Components Modulate Nucleic Acids Condensation and Phase Separation in the Context of Telomeric DNAJournal of Molecular BiologyAugust 1, 2022Andrea Soranno, J. Jeremías Incicco, Paolo De Bona, Eric J. Tomko, Eric A. Galburt, Alex S. Holehouse, Roberto Gallettobuffer T (20 mM Tris-HCl pH 8, 10% glycerol, 1 mM EDTA and 1 mM DTT) + 250 mM NaCl, applied onto a Macro-Prep High Q support (BIO-RAD) and the flow-through loaded on POROS 50 HE resin (Applied Biosystems) and eluted batch-wise

(3) Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in bloodiScienceAugust 20, 2021Matsumoto A, Takahashi Y, Ogata K, Kitamura S, Nakagawa N, Yamamoto A, Ishihama Y, Takakura Y.PS Capture™ Exosome Flow Cytometry Kit,Wako Fujifilm,297-79701 PKH26 dye,Sigma Aldrich,PKH26GL-1KT Macro-Prep®HighQ Media,Bio-Rad,1580040 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Mouse Phosphatidylserine (PS) ELISA Kit

(4) Recombinant Tumor Suppressor TSC1 Differentially Interacts with Escherichia coli DnaK and Human HSP70ACS OmegaAugust 4, 2020Nalini Natarajan, Althaf Shaik, Vijay Thiruvenkatampurchased from GE Healthcare (catalog no.27-0843-01). Bio-Rad Macro-Prep High Q resin (catalog no. 1580040) was purchased for performing AEC. The ADP-Glo assay kit was purchased from Promega Corp (Madison, WI).

(5) The 3-His Metal Coordination Site Promotes the Coupling of Oxygen Activation to Cysteine Oxidation in Cysteine DioxygenaseBiochemistryJune 2, 2020Dianna L. Forbes, Kathleen M. Meneely, Annemarie S. Chilton, Audrey L. Lamb, Holly R. EllisDifco-brand Luria-Bertani (LB) media was purchased from Becton Dickinson and Company (Sparks, MD). Macro-Prep® High Q Support column media was purchased from Bio-Rad Laboratories (Hercules, CA). Construction and purification

(6) Identification of an antilymphocyte transformation substance from Pasteurella multocidaMicrobiology and ImmunologyJuly 2, 2019Taishi Tanabe, Hiroki Fukuzawa, Yuki Amatatsu, Keigo Matsui, Hiromichi Ohtsuka, Yousuke Maeda, Hisaaki Satoat 15,000 g for 10 min at 4°C and then used as a crude extract. The crude extract was loaded onto a Macro‐Prep High Q Support (Bio‐Rad Laboratories, Hercules, CA, USA) anion exchange column (column diameter 1.0 cm, 2 mL bed volumes)

(7) ADP‐heptose is a newly identified pathogen‐associated molecular pattern ofShigella flexneriEMBO reportsDecember 1, 2018Diego García‐Weber, Anne‐Sophie Dangeard, Johan Cornil, Linda Thai, Héloïse Rytter, Alla Zamyatina, Laurence A Mulard, Cécile Arrieumerloupurification of synthetic ADP-heptose (1 mg) was performed by anion-exchange chromatography on Bio-Rad Macro-Prep High Q Support (HCOO form, column 100 × 15 mm). Elution was performed by a stepwise gradient [0.01 M to 0.25 M TEAB

(8) Proteome profiling of bovine follicular fluid-specific proteins and their effect on in vitro embryo developmentJournal of Veterinary Medical ScienceMay 3, 2017Saranya SATITMANWIWAT, Chinarat CHANGSANGFAH, Tassanee FAISAIKARM, Kornkanok PROMTHEP, Sujira THAMMAWUNG, Kulnasan SAIKHUN, Kampon KAEOKETpurify the acidic proteins, unbound proteins from the Macro-Prep High S column were loaded into the Macro-Prep High Q support in a 5-ml column (Bio-Rad) equilibrated in 10 mM Tris buffer pH 7.0. The bound proteins were also extensively

(9) Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purificationFrontiers in Plant ScienceAugust 31, 2015Laura M. López-Castillo, Janet A. I. López-Arciniega, Armando Guerrero-Rangel, Silvia Valdés-Rodríguez, Luis G. Brieba, Silverio García-Lara, Robert Winkler7.0 and centrifuged at 20,400 g for 5 min. The supernatant was collected and puriﬁed using a 5 mL “Macro-Prep High Q Support” column (BioRad, Hercules, CA, USA). The ﬂow-through was collected and concentrated using ultraﬁltration

(10) Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding proteinNucleic Acids ResearchJanuary 1, 2013Ramanagouda Ramanagoudr-Bhojappa, Lauren P. Blair, Alan J. Tackett, Kevin D. Raneyflow-through fraction. Rim1 protein was further purified by passing it through a strong anion exchange column (Macro-Prep High Q Support, Bio-Rad) with a gradient salt elution of 150 mM to 2 M NaCl. The fractions containing pure Rim1 protein

(11) The effect of conglutinin on production of reactive oxygen species in bovine granulocytesJournal of Veterinary ScienceMarch 23, 2012Marta Dec, Andrzej Wernicki, Andrzej Puchalski, Renata Urban-Chmiel, Adam WaÅ›komM EDTA). In the second step of purification, the EDTA eluate was applied to an ion exchange column (Macro-Prep High-Q Support; Bio-Rad, USA) and the proteins retained were separated by running a linear gradient of NaCl. Chromatographically

(12) Conglutinin is not specific to cattleVeterinární MedicínaNovember 11, 2011M. Dec, A. Wernicki, A. Puchalski, R. Urban-Chmiel(Bio-Rad, Poland). The protein fraction eluted with TBS-EDTA was applied to an ion-exchange column (Macro-Prep High-Q Support, Bio-Rad, Poland) and the proteins retained were separated by running a linear gradient of NaCl. Electrophoresis Electrophoresis

(13) pH stability of the stromelysin-1 catalytic domain and its mechanism of interaction with a glyoxal inhibitorBiochimica et Biophysica Acta (BBA) - Proteins and ProteomicsOctober 1, 2011Nicole Howe, Mariangela Ceruso, Edward Spink, J. Paul G. Malthouseobtained from Enzo Life Sciences (UK) Ltd., Palatine House, Matford Court, Exeter EX2 8NL, UK. The Macro-Prep High Q Support anion exchange column was obtained from Bio-Rad Laboratories, Inc. Life Science Group 2000 Alfred Nobel

(14) Cell Cycle-Related Cyclin B1 QuantificationPLoS ONESeptember 18, 2009Phyllis S. Frisa, James W. Jacobbergerconcentrated in MSI Ultrafuge or Millipore (Bedford, MA) Ultrafree-4 centrifuge filters and mixed with a Macro-Prep High Q Support (BioRad) anion exchange resin in solution A. After centrifugation the preparations were concentrated

(15) Regulation of protein kinase CK1alphaLS by dephosphorylation in response to hydrogen peroxideArchives of Biochemistry and BiophysicsOctober 15, 2007Shahinaz Bedri, Stephanie M. Cizek, Iryna Rastarhuyeva, James R. StoneB. The samples were again centrifuged at 16,000 × g for 30 min. To each sample was added 100 µL of Macro-Prep High Q Support strong anion exchange resin (Bio-Rad). The samples were incubated for 30 min at 4 °C, and then centrifuged

(16) Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon classBiochemical JournalMarch 1, 2003RafaÅ‚ SAWICKI, Sharda P. SINGH, Ashis K. MONDAL, Helen BENEÅ , Piotr ZIMNIAK(Macro-Prep DEAE Support ; BioRad Laboratories), which did not retain DmGSTE1-1. The flowthrough was then applied to a Macro-Prep High Q Support column (0.5 cmi3 cm ; Bio-Rad Laboratories), which was washed with 20 mM Tris\HCl (pH 7.5), 50 mM KCl

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