Iron Stain Kit (Prussian Blue Stain) from Abcam

Product Specs
ItemIron Stain Kit (Prussian Blue Stain)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150674
TypeStain
ApplicationsCellular Assay
TargetIron
Quantity100 test

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) The BRD4-SRPK2-SRSF2 signal modulates the splicing efficiency of ACSL3 pre-mRNA and influences erastin-induced ferroptosis in osteosarcoma cellsCell Death & DiseaseNovember 23, 2023Shun-Hong Luo, Jia-Ming Tian, Yi Chu, Hong-Yi Zhu, Jiang-Dong Ni, Jun Huangdistilled water, and drained with filter paper. The smear was stained at 37 °C for 60 min (Abcam, Cat. No. ab150674) by dripping or immersing the working solution, then thoroughly rinsed with distilled water for 5 min.

(2) High-Altitude Hypoxia Induces Excessive Erythrocytosis in Mice via Upregulation of the Intestinal HIF2a/Iron-Metabolism PathwayBiomedicinesNovember 7, 2023Sisi Zhou, Jun Yan, Kang Song, Ri-Li Gea counterstain with DAPI for 5 min. Tissue iron staining was performed with a Prussian Blue Stain (ab150674; Abcam). After deparaffinization in xylene, slides were incubated in a working Iron Stain solution for

(3) Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In VivoAntioxidants (Basel)October 30, 2023Li-Ting Tsai, Te-I Weng, Ting-Yu Chang, Kuo-Cheng Lan, Chih-Kang Chiang, Shing-Hwa LiuFormalin-fixed, paraffin-embedded kidney tissue sections were stained with an iron stain kit (Prussian Blue Stain; cat. no. ab150674, Abcam) to detect cellular iron accumulation. Briefly, potassium ferrocyanide solution and hydrochloric

(4) Drug-Loaded Lipid Magnetic Nanoparticles for Combined Local Hyperthermia and Chemotherapy against Glioblastoma MultiformeACS NanoSeptember 26, 2023Lilianne Beola, Nerea Iturrioz-Rodríguez, Carlotta Pucci, Rosalia Bertorelli, Gianni Ciofanilocalized by staining with potassium ferrocyanide (Perl’s Prussian Blue) using the Iron Stain Kit (Abcam, ab150674) according to the manufacturer’s protocol. Adjacent sections were stained with hematoxylin (H) and eosin

(5) Extravasation of Blood and Blood Toxicity Drives Tubular Injury from RBC Trapping in Ischemic AKIFunction (Oxf)September 25, 2023Sarah R McLarnon, Chloe Johnson, Jingping Sun, Qingqing Wei, Gabor Csanyi, Phillip O'Herron, Brendan Marshall, Priya Giddens, Jennifer C Sullivan, Amanda Barrett, et al.Prussian blue to detect ferrous, non-heme bound iron. ABCAM Iron Stain Kit (Prussian Blue Stain, cat# ab150674) was used for Prussian Blue staining per the manufacturer’s instructions. In brief, the slides were

(6) A ferroptosis-targeting ceria anchored halloysite as orally drug delivery system for radiation colitis therapyNat CommunAugust 22, 2023Yue Feng, Xiang Luo, Zichun Li, Xinjuan Fan, Yiting Wang, Rong-Rong He, Mingxian Liuanalysis was conducted to assess tissue iron accumulation using the Iron Stain Kit (Prussian Blue stain) (ab150674, Abcam, USA), following the manufacturer’s instructions. The iron in the tissue reacted with kit components,

(7) Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscleeLifeJune 2, 2023Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.Prussian blue iron stain (mix of potassium ferrocyanide and hydrochloric acid; Abcam, Cambridge, UK, ab150674) rinsed in water, and counterstained in nuclear fast red (Abcam, Singapore, ab150674). Iron deposits

(8) Fast detection of liver fibrosis with collagen-binding single-nanometer iron oxide nanoparticles via T 1 -weighted MRIProceedings of the National Academy of SciencesMay 2, 2023Juanye Zhang, Yingying Ning, Hua Zhu, Nicholas J. Rotile, He Wei, Himashinie Diyabalanage, Eric C. Hansen, Iris Y. Zhou, Stephen C. Barrett, Mozhdeh Sojoodi, et al.5-µm-thick slices for staining with Sirius red, hematoxylin and eosin (H&E), and Prussian Blue (Abcam, ab150674). Another piece of left lobe was quickly frozen in liquid nitrogen for hydroxyproline analysis, which

(9) Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasminProceedings of the National Academy of Sciences of the United States of AmericaJanuary 10, 2023Thepsuwan P, Bhattacharya A, Song Z, Hippleheuser S, Feng S, Wei X, Das NK, Sierra M, Wei J, Fang D, et al.enhancement, paraffin-embedded liver sections were rehydrated and stained with an iron stain (Abcam ab150674), then incubated in 3% hydrogen peroxide diluted in methanol followed by an ImmPACT DAB Substrate (Vector laboratories

(10) Preclinical Long-term Magnetic Resonance Imaging Study of Silymarin Liver-protective EffectsJournal of Clinical and Translational HepatologyDecember 28, 2022Yeon Ji Chae, Hwon Heo, Chul-Woong Woo, Sang-Tae Kim, Jae-Im Kwon, Monica Young Choi, Yu Sub Sung, Kyung Won Kim, Jeong Kon Kim, Yoonseok Choi, et al.fibrosis (stage 3), or cirrhosis (stage 4). Prussian blue staining was carried out with a staining kit (ab150674, Abcam, Cambridge, MA, USA) following the manufacturer’s protocol. Iron quantification was performed

(11) Dose-dependent benefits of iron-magnetic nanoparticle-coated human umbilical-derived mesenchymal stem cell treatment in rat intracranial hemorrhage modelStem Cell Research & TherapyDecember 1, 2022Kuan-Hung Chen, Han-Tan Chai, Kun-Chen Lin, John Y. Chiang, Pei-Hsun Sung, Chih-Hung Chen, Hon-Kan Yipironic-magnetic-nanoparticles (Ir-MNa)- coated hUC-MSCs], the Iron Stain Kit (Prussian Blue Stain) (Abcam, ab150674) was utilized in the present. The procedure and protocol were according to the manufactory instructor. Statistical

(12) Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosisCell death & diseaseOctober 29, 2022Yang M, Liu K, Chen P, Zhu H, Wang J, Huang J.distilled water, and drained with filter paper. The smear was stained at 37 °C for 60 min (Abcam, Cat. No. ab150674) by dripping or immersing the working solution, then thoroughly rinsed with distilled water for 5 min.

(13) Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodelingScientific reportsOctober 20, 2022Shin HS, Thakore A, Tada Y, Pedroza AJ, Ikeda G, Chen IY, Chan D, Jaatinen KJ, Yajima S, Pfrender EM, et al.collected 8 weeks postoperatively. Tissue sections were stained with the Prussian blue stain kit (Cat No. ab150674; Abcam, Cambridge, UK) according to the provided instructions to detect ferric iron in the tissues. Statistical

(14) Multiple types of programmed necrosis such as necroptosis, pyroptosis, oxytosis/ferroptosis, and parthanatos contribute simultaneously to retinal damage after ischemia-reperfusionScientific reportsOctober 13, 2022Dvoriantchikova G, Lypka KR, Adis EV, Ivanov D.blue stain To detect ferric iron (Fe3+) in the control and ischemic retinas, we used Iron Stain Kit (ab150674, Abcam, US) according to the manufacturer's protocol. Statistical analysis All of our experiments

(15) Ferroptosis induced by iron overload promotes fibrosis in ovarian endometriosis and is related to subpopulations of endometrial stromal cellsFrontiers in PharmacologySeptember 2, 2022Yanqin Zhang, Xinyu Liu, Mengqi Deng, Chunyu Xu, Yubo Zhang, Di Wu, Fan Tang, Ruiye Yang, Jinwei MiaoTurnbull blue (Haling, HL80082.4); Iron Assay Kit (Nanjing Jiancheng, A039-2); Prussian blue (Abcam, ab150674); FAC (Sigma Aldrich, A1332); Erastin (Topscience, T1765); Deferoxamine mesylate salt (Sigma Aldrich,

(16) Increased iron uptake in the bladder wall of racemose cysts of Taenia soliumMolecular and Biochemical ParasitologySeptember 1, 2022Miguel A Orrego, Carlos M Vasquez, Kayla Togneri, Juan P Laclette, Hector H Garcia, Theodore E Nash, Cysticercosis Working Group in Perusamples The iron content in tissue samples was determinated using the iron stain kit (Prussian blue stain) (ab150674, Abcam, Cambridge, UK). The formalin-fixed paraffin-embedded (FFPE) samples of T. solium (6 racemose

(17) Erythroid lineage Jak2V617F expression promotes atherosclerosis through erythrophagocytosis and macrophage ferroptosisJournal of Clinical InvestigationJuly 1, 2022Wenli Liu, Nataliya K Ã–stberg, Mustafa Yalcinkaya, Huijuan Dou, Kaori Endo-Umeda, Yang Tang, Xintong Hou, Tong Xiao, Trevor P. Fidler, Sandra Abramowicz, et al.reaction was developed with DAB staining (Vector Laboratories, SK-4100). The iron staining (Abcam, ab150674) was performed using a commercially available kit. In all immunofluorescence or IHC staining, isotype-matched

(18) Iron commensalism of mesenchymal glioblastoma promotes ferroptosis susceptibility upon dopamine treatmentCommunications biologyJune 16, 2022Vo VTA, Kim S, Hua TNM, Oh J, Jeong Y.normalized by protein concentration. For tissue iron staining, Iron Stain Kit (Prussian Blue Stain) (Abcam, ab150674) was used by following manufacturer’s procedure. Frozen tissues were washed with distilled water and

(19) Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease miceCell reportsJune 14, 2022Kiani Shabestari S, Morabito S, Danhash EP, McQuade A, Sanchez JR, Miyoshi E, Chadarevian JP, Claes C, Coburn MA, Hasselmann J, et al.slices taken with a Z thickness of 1 µm). The Prussian blue staining was performed using Iron stain kit (ab150674; Abcam), and Alizarin Red S (A5533, Milipore) was used for calcification labeling. Keyence BZ-X810 Widefield

(20) Coordinated missplicing of TMEM14C and ABCB7 causes ring sideroblast formation in SF3B1-mutant myelodysplastic syndromeBloodMarch 31, 2022Courtnee A. Clough, Joseph Pangallo, Martina Sarchi, Janine O. Ilagan, Khrystyna North, Rochelle Bergantinos, Massiel C. Stolla, Jasmine Naru, Patrick Nugent, Eunhee Kim, et al.12 minutes and air dried prior to iron staining. Slides were stained with Prussian Blue kit (Abcam, ab150674). Solutions were prepared according to the manufacturer’s protocol. Slides were incubated in water for

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Iron Stain Kit (Prussian Blue Stain) from Abcam

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Iron Stain Kit (Prussian Blue Stain) from Abcam

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