N-Acetyl-D-glucosamine from MilliporeSigma

Product Specs
ItemN-Acetyl-D-glucosamine
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA3286
Applicationsmammalian cell culture
Quantity100G, 25G, 5G

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Engineering E. coli strains using antibiotic-resistance-gene-free plasmidsCell Rep MethodsDecember 18, 2023Matthew B. Amrofell, Sunaina Rengarajan, Steven T. Vo, Erick S. Ramirez Tovar, Larissa LoBello, Gautam Dantas, Tae Seok Moon"M9 minimal salts, 5X",Sigma,M6030 MgCl2,Sigma,7786-30-3 MgSO4 7H2O,Sigma,63138 N-acetyl-D-glucosamine (NAcG),Sigma,A3286 Na2HPO4,Sigma,S5136 NaCl,Sigma,S7653 NAD+,Sigma,53-84-9 PEG-8000,Sigma,1546605 "Phosphate buffered

(2) Hepatocyte-specific O-GlcNAc transferase downregulation ameliorates nonalcoholic steatohepatitis by improving mitochondrial functionMolecular MetabolismSeptember 1, 2023Maria J. Gonzalez-Rellan, Tamara Parracho, Violeta Heras, Amaia Rodriguez, Marcos F. Fondevila, Eva Novoa, Natalia Lima, Marta Varela-Rey, Ana Senra, Maria D.P. Chantada-Vazquez, et al.of UDP-GlcNAc on hepatic lipid accumulation, liver cells were treated with N-Acetyl-d-glucosamine (#A3286, Sigma–Aldrich). 2.15 Oleic acid experiments In the study with oleic acid, 0.7 × 105 THLE2 and 0.3

(3) Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structuresJournal of Biological ChemistryAugust 1, 2023Robert Bucki, Daniel V. Iwamoto, Xuechen Shi, Katherine E. Kerr, Fitzroy J. Byfield, Łukasz Suprewicz, Karol Skłodowski, Julian Sutaria, Paweł Misiak, Agnieszka Z. Wilczewska, et al.Streptococcus equi mol wt 15,000 to 30,000 (97616), 1,6-Hexanediol (H11807), N-acetyl-D-glucosamine (A3286), heparin sodium salt from porcine intestinal mucosa (H3393), D-mannitol (M4125), (3-aminopropyl)trimethoxysilane

(4) Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferiPLOS GeneticsJuly 26, 2023Zhongqing Ren, Constantin N. Takacs, Hugo B. Brandão, Christine Jacobs-Wagner, Xindan Wangg/L sodium citrate (Fisher Scientific, Cat. BP327), 0.4 g/L N-acetylglucosamine (Sigma-Aldrich, Cat. A3286), 60 mL/L heat-inactivated rabbit serum (Gibco, Cat.16120), and had a final pH of 7.60. When noted,

(5) Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirementNature CommunicationsJuly 14, 2023Sourav Ghosh, Rajib Kundu, Manjunatha Chandana, Rahul Das, Aditya Anand, Subhashree Beura, Ruchir Chandrakant Bobde, Vishal Jain, Sowmya Ramakant Prabhu, Prativa Kumari Behera, et al.method. When the culture parasitemia reached around 6%, 50 mM N-acetyl-D-glucosamine (Sigma-Aldrich, A3286) was added along with 250 nM rapamycin, and continued for four days after which, the addition of N-acetyl-D-glucosamine

(6) A Plasmodium membrane receptor platform integrates cues for egress and invasion in blood forms and activation of transmission stagesScience AdvancesJune 16, 2023Ronja Marie Kuehnel, Emma Ganga, Aurélia C. Balestra, Catherine Suarez, Matthias Wyss, Natacha Klages, Lorenzo Brusini, Bohumil Maco, Nicolas Brancucci, Till S. Voss, et al.Sigma-Aldrich (31437-500G). BSA came from Sigma-Aldrich (A7906-100G). GlucNac came from (Sigma-Aldrich, A3286-100G/lot SLBX1976). Albumax medium was from GIBCO/Invitrogen (#11021-037). Hypoxanthine was from Sigma-Aldrich

(7) Targeting an essential Plasmodium cold shock protein to block growth and transmission of malaria parasiteiScienceMay 19, 2023Ankita Behl, Rumaisha Shoaib, Fernando De Leon, Geeta Kumari, Monika Saini, Evanka Madan, Vikash Kumar, Harshita Singh, Jyoti Kumari, Preeti Maurya, et al.A25742 "3,3-Diaminobenzidin",Sigma-Aldrich,Cat #D8001 N-Acetyl-D-glucosamine,Sigma-Aldrich,Cat # A3286 ProLong™ Gold Antifade Mountant with DAPI,Invitrogen™,Cat #P36935 TRIzol™ Reagent,Invitrogen™,Cat

(8) Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferibioRxivApril 9, 2023Zhongqing Ren, Constantin N. Takacs, Hugo B. Brandão, Christine Jacobs-Wagner, Xindan Wangg/L sodium citrate (Fisher Scientific, Cat. BP327), 0.4 g/L N-acetylglucosamine (Sigma-Aldrich, Cat. A3286), 60 mL/L heat-inactivated rabbit serum (Gibco, Cat.16120), and had a pH of 7.60. When noted, the following

(9) Farnesol and phosphorylation of the transcriptional regulator Efg1 affect Candida albicans white-opaque switching ratesPLOS ONEJanuary 20, 2023Lucas R. Brenes, Alexander D. Johnson, Matthew B. Lohse#291940), amino acids (2 g/L), uridine (100 μg/mL), 2% glucose, and 2% agar (SCD+aa+Uri); 2% GlcNAc (Sigma #A3286) was added instead of glucose when relevant (SCGlcNAc+aa+Uri). Farnesol plates (e.g. SCD+aa+Uri+FOH)

(10) Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitroEmerging Microbes & InfectionsDecember 31, 2022Binli Mao et al.pCMV-10 by General Biol Company (Chuzhou, China). Chloroquine (C6628) and N-acetyl-D-glucosamine (GlcN; A3286) were purchased from Sigma-Aldrich (Burlington, MA, USA) and both dissolved in sterile water. Aloxistatin

(11) Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirocheteNature communicationsNovember 22, 2022Takacs CN, Wachter J, Xiang Y, Ren Z, Karaboja X, Scott M, Stoner MR, Irnov I, Jannetty N, Rosa PA, et al.#P5280), 0.7 g/L sodium citrate (Fisher Scientific #BP327), 0.4 g/L N-acetylglucosamine (Sigma-Aldrich, #A3286), 60 mL/L heat-inactivated rabbit serum (Gibco #16120), and had a pH of 7.60. Complete BSK-H media was

(12) GDNF regulates lipid metabolism and glioma growth through RET/ERK/HIF‑1/SREBP‑1International Journal of OncologyJuly 27, 2022Zhi-Yun Yu, Hong-Jiang Li, Meng Wang, Wen-Zheng Luo, Ya-Ke Xuepyruvate (cat. no. P5280), lactate (cat. no. 1614308), D-glucose (cat. no. NIST917C), GlcNAc (cat. no. A3286), RPI-1 (cat. no. R8907), azaserine (cat. no. A4142), tunicamycin (cat. no. T7765) and OSMI-1 (cat.

(13) Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cellseLifeMarch 8, 2022Kirkemo LL, Elledge SK, Yang J, Byrnes JR, Glasgow JE, Blelloch R, Wells JA.diluted enzyme solution (0.5 µM) was combined with 100 mg/ml N-acetyl-D-glucosamine (Sigma-Aldrich, A3286-5G). Cells were allowed to sit on ice for 5 min to allow WGA to bind fully, as labeling was not altered

(14) 5-methylcytosine modification by Plasmodium NSUN2 stabilizes mRNA and mediates the development of gametocytesProceedings of the National Academy of SciencesMarch 1, 2022Meng Liu et al.red blood cell layer at the bottom of the culture dish. N-acetylglucosamine (50 mM; Sigma-Aldrich, A3286) was used for 5 d to eliminate asexual parasites (46). Giemsa-stained thin blood smears were used to

(15) Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study hostâ€“microbe cross talkSTAR ProtocolsJune 28, 2021Alex Steimle, Alessandro De Sciscio, Mareike Neumann, Erica T. Grant, Gabriel V. Pereira, Hiroshi Ohno, Eric C. Martens, Mahesh S. DesaiDMSO",Invitrogen,Cat#S7585 D-(+)-Glucose,Sigma-Aldrich,Cat#G7528 N-Acetyl-D-glucosamine,Sigma-Aldrich,Cat#A3286 "Sodium DL-lactate solution, 60% (w/w)",Sigma-Aldrich,Cat#L1375 Resazurin sodium salt,Sigma-Aldrich,Cat#199303 Hemin,Sigma-Aldrich,Cat#51280 Biotin,Sigma-Aldrich,Cat#B4501 Vitamin B12

(16) An optimized protocol for combined fluorescent lectin/immunohistochemistry to characterize tissue-specific glycan distribution in human or rodent tissuesSTAR ProtocolsMarch 1, 2021Ana Lúcia Rebelo, Paolo Contessotto, Kieran Joyce, Michelle Kilcoyne, Abhay PanditTween® 20,Thermo Fisher Scientific,85113 D-Mannose,Sigma-Aldrich,M2069 N-Acetyl-D-glucosamine,Sigma-Aldrich,A3286 N-Acetyl-D-galactosamine,Sigma-Aldrich,A2795 D-Lactose,Sigma-Aldrich,61345 L-Fucose,Sigma-Aldrich,F2252 D-Galactose,Sigma-Aldrich,G0750 Ethanol,Several

(17) Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine Bacterium Pseudoalteromonas rubra S4059Marine DrugsFebruary 12, 2021Xiyan Wang, Thomas Isbrandt, Mikael Lenz Strube, Sara Skøtt Paulsen, Maike Wennekers Nielsen, Yannick Buijs, Erwin M. Schoof, Thomas Ostenfeld Larsen, Lone Gram, Sheng-Da Zhangchitin (C9752, Sigma, St. Louis, MO, USA), 0.2% mannose (63580, Sigma, St. Louis, MO, USA), or 0.2% NAG (A3286, Sigma, St. Louis, MO, USA), respectively, as carbon source. Pre-cultures of P. rubra WT and mutants

(18) A CRISPR Interference Platform for Selective Downregulation of Gene Expression in Borrelia burgdorferiApplied and Environmental MicrobiologyJanuary 29, 2021Constantin N. Takacs, Molly Scott, Yunjie Chang, Zachary A. Kloos, Irnov Irnov, Patricia A. Rosa, Jun Liu, Christine Jacobs-Wagnersodium citrate (catalog no. BP327; Fisher Scientific), 0.4 g/liter N-acetylglucosamine (catalog no. A3286; Sigma-Aldrich) (pH 7.60), and 60 ml/liter heat-inactivated rabbit serum (catalog no. 16120; Gibco).

(19) Internalization of Garlic-Derived Nanovesicles on Liver Cells is Triggered by Interaction With CD98ACS OmegaSeptember 15, 2020Heliang Song, Brandon S. B. Canup, Vu L. Ngo, Timothy L. Denning, Pallavi Garg, Hamed Larouid-glucose (G8270), d-mannose (M8574), d-galactose (G-6404), d-fructose (F2543), N-acetylglucosamine (A3286), lactose (L3750), and maltose (M9171) were purchased from Sigma-Aldrich (St Louis, MI). Primary antibodies

(20) Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane FeedingJournal of Visualized ExperimentsJuly 28, 2020Abhai K Tripathi, Godfree Mlambo, Sachie Kanatani, Photini Sinnis, George Dimopouloswith 10x, 40x and 100x objective will work." Mosquito cups,Neptune cups,, N-acetylglucosamine,Sigma,A3286,Optional and needed only when pure gametocytes are required. Netting,,,Make sure it can contain mosquitoes

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N-Acetyl-D-glucosamine from MilliporeSigma

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N-Acetyl-D-glucosamine from MilliporeSigma

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