Spectinomycin dihydrochloride pentahydrate from MilliporeSigma

Product Specs
ItemSpectinomycin dihydrochloride pentahydrate
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS4014
Speciessynthetic (inorganic)
Applicationsplant cell culture, mammalian cell culture
Quantity25G, 5G, 5G

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coliMolecular Systems BiologyOctober 12, 2023Thorben Schramm, Paul Lubrano, Vanessa Pahl, Amelie Stadelmann, Andreas Verhülsdonk, Hannes LinkKGaA),#C0378‐25G Kanamycin,Carl Roth GmbH + Co. KG,#T832.3 Spectinomycin,Sigma‐Aldrich (Merck KGaA),#S4014‐5G l‐glycerol,Sigma‐Aldrich (Merck KGaA),#15523‐1L‐M l‐arginine,Sigma‐Aldrich (Merck KGaA),#W381918‐1KG l‐homoserine,Sigma‐Aldrich

(2) Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-CasCell Reports MethodsAugust 22, 2023Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia PiginoFischer,Cat#14000013 Paromomycin,TCI,P2092 Nourseothricin,Jena Bioscience,AB-102XL Blasticidin S,Carl Roth,CP14.2 Spectinomycin,Merck,S4014-5G EvaGreen Plus,Biotinum,31077-T GelGreen,Biotinum,41005 Betaine,Merck,61962 NEBuilder HiFi assembly

(3) A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineeringCommun BiolAugust 18, 2023Tigran V. Yuzbashev, Evgeniya Y. Yuzbasheva, Olga E. Melkina, Davina Patel, Dmitrii Bubnov, Heiko Dietz, Rodrigo Ledesma-Amarochloramphenicol (Acros Organics, 22792026), streptomycin (Sigma-Aldrich, S9137), spectinomycin (Sigma-Aldrich, S4014), nourseothricin (Fisher Scientific, AB-101-10ML), and hygromycin (Life Technologies, 10687010) are

(4) Modularity and diversity of target selectors in Tn7 transposonsMolecular CellJune 15, 2023Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova, et al.anhydrous basis",Millipore sigma,C1389 Spectinomycin dihydrochloride pentahydrate,Millipore sigma,S4014 Kanamycin sulfate from Streptomyces kanamyceticus,Sigma,K4000 Chloramphenicol,Sigma,C0378 MiSeq Reagent

(5) pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombinationHeliyonJune 1, 2023S. Boukthir, P. Gaudu, A. Faili, S. Kayalcultured in Todd Hewitt medium supplemented with 0.2% yeast extract (THY) and spectinomycin (SPC) (S4014, Sigma-Aldrich™) when required. All bacterial cultures of GAS were performed with 5% CO2 at indicated

(6) Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cellsThe Journal of cell biologyMarch 6, 2023Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanović V, Omi S, Pedelacq JD, Iv F, et al.purification are as follows. E. coli BL21(DE3) from Agilent (200131). Carbenicillin (C3416), spectinomycin (S4014), LB broth medium (L3022), LB agar (L2897), SOC medium (S1797) from Sigma-Aldrich. Terrific Broth from

(7) Antibiotic perseverance increases the risk of resistance developmentProceedings of the National Academy of SciencesJanuary 10, 2023Gerrit Brandis, Jimmy Larsson, Johan Elf carbenicillin (C1389), ceftriaxone (C5793), ciprofloxacin (PHR1044), gentamycin (G1914), and spectinomycin (S4014), in 70% ethanol for chloramphenicol (C0378) and tetracycline (T7660), in methanol for rifampicin (R3501),

(8) Assembly dynamics of FtsZ and DamX during infection-related filamentation and division in uropathogenic E. coliNature CommunicationsDecember 1, 2022Bill SÃ¶derstrÃ¶m, Matthew J. Pittorino, Daniel O. Daley, Iain G. DugginAntibiotics were added when needed (ampicillin 100 μg ml−1 (Sigma #A9518), spectinomycin 25 μg ml−1 (Sigma #S4014)). The following morning, the cultures were pelleted and resuspended in 1× PBS (Bio-Rad #1610780) to

(9) Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancersNature CommunicationsDecember 1, 2022Renata Bordeira-Carriço et al.ThermoFisher Scientific), grown O.N. at 37 °C. Vector selection was performed with 100 μg/ml Spectinomycin (#S4014, Sigma-Aldrich) in the growth medium for the pCR®8/GW/TOPO vectors, or 100 μg/ml Ampicillin (#624619.1,

(10) Host-dependent resistance of Group A Streptococcus to sulfamethoxazole mediated by a horizontally-acquired reduced folate transporterNature CommunicationsNovember 30, 2022M. Kalindu D. Rodrigo, Aarti Saiganesh, Andrew J. Hayes, Alisha M. Wilson, Jack Anstey, Janessa L. Pickering, Jua Iwasaki, Jessica Hillas, Scott Winslow, Tabitha Woodman, et al.Luria–Bertani medium (LB; Thermo Fisher 22700025 and 12780052). As required, spectinomycin (Sigma Aldrich S4014) was used at 100 μg/ml for both GAS and E. coli. Antibiotic susceptibility testing Etest assays were

(11) Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virusiScienceNovember 3, 2022Phu-Tri Tran, Mi-Sa Vo Phan, Vitaly CitovskyS,Sigma-Aldrich,#P7170 Casein,Sigma-Aldrich,#C8654-500G TBS-T,Sigma-Aldrich,#T9039-10PAK Kanamycin,British Pharmacopoeia,#BP861 Spectinomycin,Sigma-Aldrich,#S4014-5G LB broth,Sigma-Aldrich,#L3022-250G ,, Critical commercial assays,Critical commercial assays,Critical

(12) Glutamate catabolism during sporulation determines the success of the future spore germinationiScienceOctober 21, 2022Lei Rao, Bing Zhou, Raphael Serruya, Arieh Moussaieff, Lior Sinai, Sigal Ben-YehudaK0010 Lincomycin,Sigma-Aldrich,Cat#: 62143-5G Erythromycin,Sigma-Aldrich,Cat#: E0774 Spectinomycin,Sigma-Aldrich,Cat#: S4014-5G Ampicillin,Sigma-Aldrich,Cat#: A9518-25G L-Alainine,MP BIOMEDICALS,Cat#: 100287 Difco™ Nutrient Broth

(13) An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell linesSTAR ProtocolsJune 17, 2022Bibekananda Kar, Ankit Sabharwal, Santiago Restrepo-Castillo, Brandon W. Simone, Karl J. Clark, Stephen C. EkkerBioscience,Cat#BIO-33053 Kanamycin sulfate,Sigma-Aldrich,Cat#K4000 Spectinomycin dihydrochloride pentahydrate,Sigma-Aldrich,Cat#S4014 LB broth,Fisher Scientific,Cat#BP1426-500 Agar,BD Biosciences,Cat#VWR 90000-762 Plasmid-Safe ATP-Dependent

(14) An Improved Agrobacterium-Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature EmbryosFrontiers in Plant ScienceMay 4, 2022Minjeong Kang, Keunsub Lee, Todd Finley, Hal Chappell, Veena Veena, Kan Wangmg/mL)*,50 mg/L,"Millipore Sigma, K1377" and/or,spectinomycin (100 mg/mL)*,100 mg/L,"Millipore Sigma, S4014" ,,, Working plate,sodium chloride,5 g/L,"Fisher scientific, S271" YEP base,yeast extract,5 g/L,"Fisher

(15) Site-specific nanobody-oligonucleotide conjugation for super-resolution imagingJournal of Biological MethodsMarch 1, 2022Laura Teodori, Marjan Omer, Anders Märcher, Mads K. K. Skaanning, Veronica L. L. Andersen, Jesper S. S. Nielsen, Emil Oldenburg, Yuchen Lin, Kurt V. V. Gothelf, Jørgen Kjems#A9518-25G) stock solution: 50 and 100 mg/ml, sterile-filtered✓ Spectinomycin (SPEC) (Sigma Aldrich, Cat. #S4014-5G) stock solution: 50 and 100 mg/ml, sterile-filtered✓ Isopropyl β-D-1-thiogalactopyranoside (IPTG)

(16) Genetically stable CRISPR-based kill switches for engineered microbesNature CommunicationsFebruary 3, 2022Austin G. Rottinghaus, Aura Ferreiro, Skye R. S. Fishbein, Gautam Dantas, Tae Seok MoonPBS, and plated on LB agar supplemented with 100 μg/mL spectinomycin dihydrochloride pentahydrate (S4014, Sigma-Aldrich) to select for spectinomycin-resistant kill switch strains, and 34 μg/mL chloramphenicol

(17) Production and secretion of functional SARS-CoV-2 spike protein in Chlamydomonas reinhardtiiFrontiers in plant scienceJanuary 1, 2022Kiefer AM, Niemeyer J, Probst A, Erkel G, Schroda M.2021) with constructs linearized with NotI. Transformants were selected on 100 μg/mL spectinomycin (S4014, Sigma-Aldrich). Cell counts were performed with a Z2 Coulter Counter (Beckman). To validate the functionality

(18) Regulation of prophage induction and lysogenization by phage communication systemsCurrent BiologyNovember 22, 2021John B. Bruce, Sébastien Lion, Angus Buckling, Edze R. Westra, Sylvain GandonAgar,Formedium,cat#LMM02 MnCl2,Sigma-Aldrich,cat#244589 MgCL2,Sigma-Aldrich,cat#M8266 Spectinomycin,Sigma-Aldrich,cat#S4014 Kanamycin,Sigma-Aldrich,cat#B5264 Mannose,Sigma-Aldrich,cat#M6020 Xylose,Sigma-Aldrich,cat#X1500 BsaI_HFv2,New England Biolabs

(19) The arbitrium system controls prophage inductionCurrent BiologyNovember 22, 2021Aisling Brady, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Sara Zamora-Caballero, Alonso Felipe-Ruíz, Jorge Val-Calvo, Wilfried J.J. Meijer, Alberto Marina, José R. PenadésL3022-1KG Agar,Formedium,Cat. # AGA02 Spectinomycin dihydrochloride pentahydrate,Sigma – Aldrich,Cat. # S4014-5G Erythromycin,Sigma – Aldrich,Cat. # E6376-25G Kanamycin Sulfate,Sigma – Aldrich,Cat. # 60615-5G Ampicillin Sodium Salt

(20) Enrichment of plasmid pool from E. coli for mass sequencing to detect untargeted mutagenic eventsSTAR ProtocolsJune 18, 2021Asako Isogawa, Robert P. Fuchs, Shingo FujiiAmpicillin sodium salt,Sigma-Aldrich,Cat#A9518-5G Kanamycin sulfate,Sigma-Aldrich,Cat#60615-5G Spectinomycin dihydrochloride pentahydrate,Sigma-Aldrich,Cat#S4014-5G Chloramphenicol,Sigma-Aldrich,Cat#C0378-5G ,, Critical commercial assays,Critical commercial assays,Critical

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Spectinomycin dihydrochloride pentahydrate from MilliporeSigma

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Spectinomycin dihydrochloride pentahydrate from MilliporeSigma

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